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重組人胰島素溶液(10 mg/mL)
貨號:PB180432
價格:¥2409601500
- 1mL
- 1mLx5
- 1mLx10
產(chǎn)品概述
胰島素(Insulin,INS)是由胰島β細(xì)胞分泌的一種雙鏈形式(α, β)的多肽激素,不同物種動物(人、牛、羊、豬等)的胰島素功能大體相同,成分稍有差異,胰島素是目前已知的機(jī)體內(nèi)唯一降低血糖的激素,也是唯一同時促進(jìn)糖原、脂肪、蛋白質(zhì)合成的激素。胰島素在許多細(xì)胞活動中發(fā)揮重要作用,是哺乳動物細(xì)胞培養(yǎng)基中常用的添加物質(zhì)胰島素能加速葡萄糖的利用和抑制葡萄糖的生成,抑制脂肪的分解,促進(jìn)脂肪的合成和貯存,促進(jìn)蛋白質(zhì)的合成,阻止蛋白質(zhì)的分解,促進(jìn)鉀離子和鎂離子穿過細(xì)胞膜進(jìn)入細(xì)胞內(nèi);促進(jìn)脫氧核糖核酸(DNA)、核糖核酸(RNA)及三磷酸腺苷(ATP)的合成。
普諾賽生產(chǎn)的重組人胰島素(Recombinant Human Insulin),分子量約為5800 Da,結(jié)構(gòu)與天然來源的人胰島素相同,常用作細(xì)胞培養(yǎng)的輔助成分,常見使用濃度為1 μg/mL-10 μg/mL。
產(chǎn)品信息
形態(tài) | 液體 |
規(guī)格 | 1mL/1mLx5/1mLx10 |
pH | 2.0-3.5 |
重組人胰島素 | 10 mg/mL |
活性 | ≥275 IU/mL |
儲存條件 | -5~-20℃,避光 |
運(yùn)輸條件 | 低溫 |
有效期 | 12個月 |
注意事項
1、本產(chǎn)品經(jīng)0.1 μm過濾除菌,使用本產(chǎn)品時應(yīng)注意無菌操作,避免污染;
2、本產(chǎn)品為10 mg/mL濃縮液,請根據(jù)需要稀釋后使用;
3、2-8℃中解凍,混勻后使用,切忌反復(fù)凍融,用量較少時建議分裝凍存,2-8℃中保存,至少6個月有效;
4、不宜長時間放置于室溫或較高溫度的環(huán)境中;
5、溶解后若有沉淀產(chǎn)生,待溶液升至室溫后混勻即可溶解;若遇無法溶解時,用無菌的0.1 M HCl緩慢調(diào)節(jié)pH至2-3之間(邊調(diào)節(jié),邊用pH試紙檢測pH);若仍然無法溶解,請及時與我們聯(lián)系;
6、本產(chǎn)品僅用于科研或進(jìn)一步研究使用,不用于診斷和治療。
參考文獻(xiàn)
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期刊:ACS Applied Materials & Interfaces
影響因子 :9.5
引用產(chǎn)品: MCF 10A細(xì)胞專用培養(yǎng)基 , DMEM/F12 (含HEPES) 培養(yǎng)基 , 馬血清 , 重組人胰島素溶液 (10mg/mL)
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Macroscopic Supramolecular Assembly Strategy to Construct 3D Biocompatible Microenvironments with Site-Selective Cell Adhesion (2021-06-11)
期刊:ACS Applied Materials & Interfaces
影響因子 :9.5
引用產(chǎn)品: MG-63 細(xì)胞 , PC-12 (Undifferentiated) 細(xì)胞 , 重組人胰島素溶液 (10mg/mL) , MEM非必需氨基酸溶液 (NEAA),100× , PUMC-HUVEC-T1 , CCC-SMC-1 細(xì)胞
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Metformin regulates adiponectin signalling in epicardial adipose tissue and reduces atrial fibrillation vulnerability (2020-05-22)
期刊:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影響因子 :4.5
引用產(chǎn)品: 重組人胰島素溶液(10 mg/mL)
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MiR-519d and miR-328-3p Combinatorially Suppress Breast Cancer Progression (2020-12-18)
期刊:OncoTargets and Therapy
影響因子 :4.0
引用產(chǎn)品: Leibovitz's L-15 培養(yǎng)基 , 特級胎牛血清 , 青霉素-鏈霉素溶液 (雙抗),100× , MEM (含NEAA) 培養(yǎng)基 , 重組人胰島素溶液(10 mg/mL) , McCoy's 5A 培養(yǎng)基 , DMEM/F12 培養(yǎng)基 , McCoy's 5A 培養(yǎng)基 , DMEM/F12 培養(yǎng)基 , MEM 培養(yǎng)基 , 青霉素-鏈霉素溶液(雙抗),100× , 特級胎牛血清 , Leibovitz's L-15 培養(yǎng)基
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