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產品概述
名稱 | HCC1954 (人乳腺導管癌細胞) (STR鑒定正確) |
別稱 | HCC-1954; Hamon Cancer Center 1954 |
種屬 | 人 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 上皮細胞樣,有空泡 |
凍存條件 | 凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:4-1:8 |
推薦換液頻率 | 2-3次/周 |
背景描述 | HCC1954 is an epithelial cell line isolated from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases from a 61-year-old, Asian female in 1995. HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). Her2/neu is overexpressed in the ELISA assay. |
年齡(性別) | 女性;61歲 |
組織來源 | Breast; Duct; Mammary gland |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 乳腺癌細胞 |
生物安全等級 | BSL-1 |
倍增時間 | 45 hours (PubMed=25984343); 43.81 hours (GrayJW panel) |
受體表達情況 | Estrogen receptor, not expressed; progesterone receptor, not expressed |
基因表達情況 | epithelial glycoprotein 2 (EGP2); cytokeratin 19 |
保藏機構 | ATCC; CRL-2338 BCRJ; 0280 KCLB; 9S1954 |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 10 D2S1338 17,26 D3S1358 15,16 D5S818 11 D7S820 10,11 D8S1179 12,15 D13S317 8,9 D16S539 9,11 D18S51 14,18 D19S433 13,15.2 D21S11 28,32.2 FGA 22,23 PentaD 9,12 PentaE 12,16 TH01 6,7 TPOX 8,9 vWA 18,19 D6S1043 11,14 D12S391 22 D2S441 10,11 -
STR鑒定圖
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參考文獻
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SLC31A1 promotes chemoresistance through inducing CPT1A-mediated fatty acid oxidation in ER-positive breast cancer (2025-02-03)
期刊:NEOPLASIA
影響因子 :6.3
引用產品: ZR-75-1 細胞 , HCC1954 細胞 , MDA-MB-453 細胞 , 青霉素-鏈霉素溶液(雙抗),100×
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ERBB3-related gene PBX1 is associated with prognosis in patients with HER2-positive breast cancer (2025-01-10)
期刊:BMC Genomic Data
DOI:10.1186/s12863-024-01292-0
影響因子 :1.9
引用產品: MCF 10A 細胞 , HCC1954 細胞 , BT-474 [BT474] 細胞
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Tanshinone I improves TNBC chemosensitivity by suppressing late-phase autophagy through AKT/p38 MAPK signaling pathway (2024-07-02)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2024.117037
影響因子 :6.9
引用產品: HCC1937 細胞 , HCC1954 細胞 , 重組人胰島素溶液(10 mg/mL)
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