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HCE-T (人角膜上皮細(xì)胞) (STR鑒定正確)
貨號(hào):CL-0743
規(guī)格:1×10?cells/T25培養(yǎng)瓶
價(jià)格: ¥2000
生長(zhǎng)培養(yǎng)基:DMEM/F12+ 15% FBS+ 5μg/ml Insulin + 10ng/ml human EGF+1% P/S
產(chǎn)品概述
名稱 | HCE-T (人角膜上皮細(xì)胞) (STR鑒定正確) |
別稱 | HCET; HCE; SV40-HCEC |
種屬 | 人 |
生長(zhǎng)特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
生長(zhǎng)培養(yǎng)基:
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:3 |
推薦換液頻率 | 2-3天/次 |
注意事項(xiàng) | 該細(xì)胞匯合度在90%以下時(shí)消化時(shí)間不超過(guò)8min,匯合度在90%以上時(shí),消化時(shí)間在10min以上。 |
背景描述 | SV40-Adeno vector transformed cornea cells. 64kD keratin producing. Choleratoxin-free medium. |
年齡(性別) | 女性;49歲 |
組織來(lái)源 | 角膜 |
細(xì)胞類型 | 轉(zhuǎn)化細(xì)胞系 |
生物安全等級(jí) | BSL-2 |
倍增時(shí)間 | ~49 hours |
保藏機(jī)構(gòu) | RCB; RCB2280 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 12 D2S1338 19,20 D3S1358 17 D5S818 13 D7S820 10,13 D8S1179 11,13 D13S317 8 D16S539 9,11 D18S51 13,15 D19S433 13,14 D21S11 27,30 FGA 20 PentaD 12,15 PentaE 15,16 TH01 7,8 TPOX 8,11 vWA 18,19 D6S1043 18,19 D12S391 18,19 D2S441 10 -
STR鑒定圖
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參考文獻(xiàn)
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Identification of glutamine as a potential therapeutic target in dry eye disease (2025-01-22)
期刊:Signal Transduction and Targeted Therapy
DOI:10.1038/s41392-024-02119-1
影響因子 :40.8
引用產(chǎn)品: HCE-T 細(xì)胞
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A Silk Fibroin Nanoparticle Hydrogel Loaded With NK1R Antagonist Has Synergistic Anti-Inflammatory and Reparative Effects on Dry Eye Disease (2025-02-22)
期刊:Advanced Science
影響因子 :14.3
引用產(chǎn)品: HCE-T 細(xì)胞 , 重組人胰島素溶液(10 mg/mL)
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Ferroptosis and PANoptosis under hypoxia pivoting on the crosstalk between DHODH and GPX4 in corneal epithelium (2025-01-04)
期刊:FREE RADICAL BIOLOGY AND MEDICINE
DOI:10.1016/j.freeradbiomed.2024.12.050
影響因子 :7.1
引用產(chǎn)品: HCE-T 細(xì)胞 , 293T [HEK-293T] 細(xì)胞
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Cyclic RGD conjugated PEG-PLGA nanoparticle enhances preventative effect of bilirubin on the development of ultraviolet-B (UVB)-induced cataract (2024-08-22)
期刊:CHEMICAL ENGINEERING JOURNAL
影響因子 :13.3
引用產(chǎn)品: HCE-T 細(xì)胞
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Identification of LRRC46 as a novel candidate gene for high myopia (2024-06-11)
期刊:Science China-Life Sciences
影響因子 :8.0
引用產(chǎn)品: 重組人胰島素溶液(10 mg/mL) , HFSF 細(xì)胞 , 人鞏膜成纖維細(xì)胞完全培養(yǎng)基 , 磷酸鹽緩沖液(PBS) 1× , HCE-T 細(xì)胞 , 特級(jí)胎牛血清 , EGF/Urogastrone/URG, Human, Recombinant
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Toxicological responses of A549 and HCE-T cells exposed to fine particulate matter at the air–liquid interface (2024-03-21)
期刊:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
DOI:10.1007/s11356-024-32944-4
影響因子 :5.8
引用產(chǎn)品: HCE-T 細(xì)胞
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Exosomes derived from adipose mesenchymal stem cells promote corneal injury repair and inhibit the formation of scars by anti-apoptosis (2024-12-12)
期刊:COLLOIDS AND SURFACES B-BIOINTERFACES
DOI:10.1016/j.colsurfb.2024.114454
影響因子 :5.4
引用產(chǎn)品: HCE-T 細(xì)胞
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cRGD-Conjugated Bilirubin Nanoparticles Alleviate Dry Eye Disease Via Activating the PINK1-Mediated Mitophagy (2024-11-04)
期刊:INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
影響因子 :5.0
引用產(chǎn)品: RAW 264.7 細(xì)胞 , HCE-T 細(xì)胞
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Caffeine’s protective role in dry eye disease and meibomian gland Dysfunction: Insights from clinical and Experimental models (2024-12-21)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.113863
影響因子 :4.8
引用產(chǎn)品: HCE-T 細(xì)胞
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A Self-Generated Electricity-Driven Drug Delivery System for Precision Management of Myopia (2023-10-28)
期刊:Nano Energy
DOI:10.1016/j.nanoen.2023.109040
影響因子 :17.6
引用產(chǎn)品: HCE-T 細(xì)胞 , 人淋巴管內(nèi)皮細(xì)胞
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In vitro validation: GLY alleviates UV-induced corneal epithelial damage through the HMGB1-TLR/MyD88-NF-κB signaling pathway (2023-11-06)
期刊:ACTA HISTOCHEMICA
DOI:10.1016/j.acthis.2023.152111
影響因子 :2.5
引用產(chǎn)品: HCE-T 細(xì)胞
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