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大鼠骨髓間充質(zhì)干細(xì)胞
Rat Bone Marrow Mesenchymal Stem Cells
貨號(hào):CP-R131
規(guī)格:5×10?Cells/T25培養(yǎng)瓶
價(jià)格: ¥3380
培養(yǎng)基貨號(hào): CM-R131
產(chǎn)品概述
大鼠骨髓間充質(zhì)干細(xì)胞
Cat NO.: CP-R131
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產(chǎn)品名稱:大鼠骨髓間充質(zhì)干細(xì)胞
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英文簡稱:BMSCs,MSCs
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組織來源:骨髓
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產(chǎn)品規(guī)格:5×10?Cells/T25培養(yǎng)瓶
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細(xì)胞簡介:
大鼠骨髓間充質(zhì)干細(xì)胞(BMSC)分離自骨髓;骨髓是機(jī)體的造血組織,位于身體的許多骨骼內(nèi)。成年動(dòng)物的骨髓分兩種:紅骨髓和黃骨髓。紅骨髓能制造紅細(xì)胞、血小板和各種白細(xì)胞。血小板有止血作用,白細(xì)胞能殺滅與抑制各種病原體,包括細(xì)菌、病毒等;某些淋巴細(xì)胞能制造抗體。因此,骨髓不但是造血器官,它還是重要的免疫器官。骨髓是存在于長骨(如肱骨、股骨)的骨髓腔和扁平骨(如髂骨)的稀松骨質(zhì)間的網(wǎng)眼中,是一種海綿狀的組織,能產(chǎn)生血細(xì)胞的骨髓略呈紅色,稱為紅骨髓。出生時(shí),紅骨髓充滿全身骨髓腔,隨著年齡增大,脂肪細(xì)胞增多,相當(dāng)部分紅骨髓被黃骨髓取代,最后幾乎只有扁平骨骨髓腔中有紅骨髓。骨髓基質(zhì)系統(tǒng)內(nèi)存在的骨髓間充質(zhì)干細(xì)胞是一種除造血干細(xì)胞以外的、具有高度自我更新能力和多向分化潛能的干細(xì)胞,可以向骨、軟骨、肌組織、皮膚、脂肪、神經(jīng)等多種組織分化,因此可以作為組織工程中的種子細(xì)胞。在骨髓中,BMSC占骨髓有核細(xì)胞總數(shù)的0.001%-0.1%,含量極低。骨髓間充質(zhì)干細(xì)胞的體外培養(yǎng)條件要求較高,在培養(yǎng)過程中,受貼壁時(shí)間、種植密度、血清含量、培養(yǎng)溫度和培養(yǎng)基pH值等條件的影響。
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方法簡介:
普諾賽實(shí)驗(yàn)室分離的大鼠骨髓間充質(zhì)干細(xì)胞采用沖洗骨髓、密度梯度離心、差速貼壁法結(jié)合培養(yǎng)基篩選制備而來,細(xì)胞總量約為5×10?cells/瓶。
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質(zhì)量檢測(cè):
普諾賽實(shí)驗(yàn)室分離的大鼠骨髓間充質(zhì)干細(xì)胞經(jīng)CD90或CD44免疫熒光鑒定,純度可達(dá)90%以上,且不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌等。
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培養(yǎng)信息:
包被條件 培養(yǎng)基 含F(xiàn)BS、生長添加劑、Penicillin、Streptomycin等 產(chǎn)品貨號(hào) CM-R131 換液頻率 每2-3天換液一次 生長特性 貼壁 細(xì)胞形態(tài) 成纖維細(xì)胞樣 傳代特性 可傳5代左右;3代以內(nèi)狀態(tài)最佳 消化液 0.25%胰蛋白酶 大鼠骨髓間充質(zhì)干細(xì)胞體外培養(yǎng)周期有限;建議使用普諾賽配套的專用生長培養(yǎng)基及正確的操作方法來培養(yǎng),以此保證該細(xì)胞的最佳培養(yǎng)狀態(tài)。
參考文獻(xiàn)
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Development of a Novel Magnesium Alloy with High Degradation Resistance and Osteo/Angiogenesis Activity Through Scandium-enhanced Growth of Passivation Film (2025-01-10)
期刊:ADVANCED FUNCTIONAL MATERIALS
影響因子 :18.5
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞 , 人臍靜脈內(nèi)皮細(xì)胞
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Dual-target nanotherapy for vascular endothelium and bone mesenchymal stem cells halt steroid-induced osteonecrosis of the femoral head progression (2025-02-05)
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2024.12.081
影響因子 :10.5
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞
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Sea-Island Micelle Structured Hydrogel Scaffold: A Dual-Action Approach to Combat Cartilage Damage under RA Conditions (2025-01-02)
期刊:ACS Applied Materials & Interfaces
影響因子 :8.3
引用產(chǎn)品: 大鼠軟骨細(xì)胞 , RAW 264.7 細(xì)胞 , 大鼠骨髓間充質(zhì)干細(xì)胞
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Androgen deficiency-induced loss of Lactobacillus salivarius extracellular vesicles is associated with the pathogenesis of osteoporosis (2025-01-09)
期刊:MICROBIOLOGICAL RESEARCH
DOI:10.1016/j.micres.2025.128047
影響因子 :6.1
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Enhanced mechanical, antibacterial, and osteogenic performance of zinc-doped micro-nano porous layer on Ti6Al4V: Realized by ion exchange and induction heating (2025-01-10)
期刊:SURFACE & COATINGS TECHNOLOGY
DOI:10.1016/j.surfcoat.2025.131763
影響因子 :5.3
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Metal ion-crosslinking multifunctional hydrogel microspheres with inflammatory immune regulation for cartilage regeneration (2025-01-28)
期刊:Frontiers in Bioengineering and Biotechnology
DOI:10.3389/fbioe.2025.1540592
影響因子 :4.3
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Novel Decellularization Scheme for Preparing Acellular Fish Scale Scaffolds for Bone Tissue Engineering (2025-01-01)
期刊:ACS Omega
影響因子 :3.7
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Injectable and in situ foaming shape-adaptive porous Bio-based polyurethane scaffold used for cartilage regeneration (2024-05-13)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2024.03.012
影響因子 :18.9
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An injectable and coagulation-independent Tetra-PEG hydrogel bioadhesive for post-extraction hemostasis and alveolar bone regeneration (2024-03-19)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2024.03.015
影響因子 :18.9
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Modular scaffolds with intelligent visual guidance system for in situ bone tissue repair (2024-11-29)
期刊:International Journal of Extreme Manufacturing
影響因子 :16.1
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An anti-infection and biodegradable TFRD-loaded porous scaffold promotes bone regeneration in segmental bone defects -experimental studies (2024-03-19)
期刊:International Journal of Surgery
DOI:10.1097/JS9.0000000000001291
影響因子 :15.3
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期刊:Nature Communications
DOI:10.1038/s41467-024-48417-8
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DOI:10.1016/j.biomaterials.2024.122699
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Engineered dECM-based microsystem promotes cartilage regeneration in osteoarthritis by synergistically enhancing chondrogenesis of BMSCs and anti-inflammatory effect (2024-11-13)
期刊:COMPOSITES PART B-ENGINEERING
DOI:10.1016/j.compositesb.2024.111974
影響因子 :12.7
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In situ preparation of nano cone-like structures on 3D printed titanium alloy implants via one-step femtosecond laser manufacturing for better osseointegration, anti-corrosion, and anti-fatigue (2024-04-25)
期刊:JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
DOI:10.1016/j.jmst.2024.03.070
影響因子 :10.9
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Functionalized 3D Hydroxyapatite Scaffold by Fusion Peptides-Mediated Small Extracellular Vesicles of Stem Cells for Bone Tissue Regeneration (2024-01-12)
期刊:ACS Applied Materials & Interfaces
影響因子 :9.5
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A Photothermal Responsive System Accelerating Nitric Oxide Release to Enhance Bone Repair by Promoting Osteogenesis and Angiogenesis (2024-08-10)
期刊:Materials Today Bio
DOI:10.1016/j.mtbio.2024.101180
影響因子 :8.7
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Multifunctional tannic acid-based nanocomposite methacrylated silk fibroin hydrogel with the ability to scavenge reactive oxygen species and reduce inflammation for bone regeneration (2024-04-03)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.131357
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A chondroitin sulphate hydrogel with sustained release of SDF-1α for extensive cartilage defect repair through induction of cell homing and promotion of chondrogenesis (2024-08-08)
期刊:Journal of Materials Chemistry B
影響因子 :6.1
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Coatless modification of 3D-printed Ti6Al4V implants through tailored Cu ion implantation combined with UV photofunctionalization to enhance cell attachment, osteogenesis and angiogenesis (2024-04-06)
期刊:COLLOIDS AND SURFACES B-BIOINTERFACES
DOI:10.1016/j.colsurfb.2024.113891
影響因子 :5.8
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A facile nanopattern modification of silk fibroin electrospun scaffold and corresponding impact on cell proliferation and osteogenesis (2024-10-01)
期刊:Regenerative Biomaterials
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Hydroxyapatite Nanoparticles Promote the Development of Bone Microtissues for Accelerated Bone Regeneration by Activating the FAK/Akt Pathway (2024-06-07)
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DOI:10.1021/acsbiomaterials.4c00574
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DOI:10.1016/j.jddst.2024.106401
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期刊:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
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DOI:10.1007/s13577-024-01086-7
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期刊:Frontiers in Bioscience-Landmark
影響因子 :3.3
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Based on NF-κB and Notch1/Hes1 Signaling Pathways, the Mechanism of Artesunate on Inflammation in Osteoporosis in Ovariectomized Rats was Investigated (2024-07-24)
期刊:Frontiers in Bioscience-Landmark
影響因子 :3.3
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Preliminary study of the role of histone deacetylase (HDAC) in steroid-induced avascular necrosis of the femoral head induced by BMSC adipogenic differentiation (2024-10-12)
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DOI:10.1186/s13018-024-05121-z
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Mesenchymal stem cell-derived exosomal mir-21-5p inhibits YAP1 expression and improves outcomes in myocardial infarction (2024-10-10)
期刊:BMC Cardiovascular Disorders
DOI:10.1186/s12872-024-04197-z
影響因子 :2.0
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期刊:COMPOSITES PART B-ENGINEERING
DOI:10.1016/j.compositesb.2023.110620
影響因子 :13.1
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期刊:COMPOSITES PART B-ENGINEERING
DOI:10.1016/j.compositesb.2023.111147
影響因子 :13.1
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Inducing in situ M2 macrophage polarization to promote the repair of bone defects via scaffold-mediated sustained delivery of luteolin (2023-12-18)
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2023.11.015
影響因子 :10.8
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Biomimetic composite hydrogel promotes new bone formation in rat bone defects through regulation of miR-19b-3p/WWP1 axis by loaded extracellular vesicles (2023-11-30)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-023-02201-w
影響因子 :10.2
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期刊:Advanced Healthcare Materials
影響因子 :10.0
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The biological properties of 3D-printed degradable magnesium alloy WE43 porous scaffolds via the oxidative heat strategy (2023-02-15)
期刊:International Journal of Bioprinting
DOI:10.18063/ijb.686
影響因子 :8.4
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2D silicene nanosheets-loaded coating for combating implant-associated infection (2023-10-21)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2023.127585
影響因子 :8.2
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞
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Bi-lineage inducible and immunoregulatory electrospun fibers scaffolds for synchronous regeneration of tendon-to-bone interface (2023-07-26)
期刊:Materials Today Bio
DOI:10.1016/j.mtbio.2023.100749
影響因子 :8.2
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞 , RAW 264.7 細(xì)胞
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Effect of La3+ and Mg2+ combined system on bioactivity and osteogenesis of bioinspired La-doped magnesium phosphate composites prepared utilizing the precursor method (2023-05-19)
期刊:Journal of Materials Research and Technology-JMR&T
DOI:10.1016/j.jmrt.2023.05.133
影響因子 :6.4
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Bioinspired strontium magnesium phosphate cement prepared utilizing the precursor method for bone tissue engineering (2023-02-03)
期刊:Frontiers in Bioengineering and Biotechnology
DOI:10.3389/fbioe.2023.1142095
影響因子 :5.7
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In situ fabrication of an anisotropic double-layer hydrogel as a bio-scaffold for repairing articular cartilage and subchondral bone injuries (2023-11-30)
期刊:RSC Advances
影響因子 :3.9
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞
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PDE-5-Inhibited BMSCs Alleviate High Glucose-Induced Myocardial Fibrosis and Cardiomyocyte Apoptosis by Activating the cGMP/PKG Pathway (2023-07-28)
期刊:Frontiers in Bioscience-Landmark
影響因子 :3.1
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Exploring the mechanism of Astragali radix for promoting osteogenic differentiation based on network pharmacology, molecular docking, and experimental validation (2023-09-10)
期刊:Chemical Biology & Drug Design
影響因子 :3.0
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞
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Exosome-shuttled MYCBPAP from bone marrow mesenchymal stem cells regulates synaptic remodeling and ameliorates ischemic stroke in rats (2023-07-07)
期刊:JOURNAL OF CHEMICAL NEUROANATOMY
DOI:10.1016/j.jchemneu.2023.102309
影響因子 :2.8
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞 , 大鼠骨髓間充質(zhì)干細(xì)胞完全培養(yǎng)基 , 大鼠皮層神經(jīng)元細(xì)胞 , DMEM無糖 培養(yǎng)基 , Annexin V-FITC/ PI熒光雙染細(xì)胞凋亡檢測(cè)試劑盒
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A New Method Regulates Bone Fracture Tissue Exosome lncRNA-mRNA to Promote Mesenchymal Stem Cell Proliferation and Migration (2023-11-14)
期刊:INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
DOI:10.1016/j.injury.2023.111210
影響因子 :2.5
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞 , 大鼠骨髓間充質(zhì)干細(xì)胞完全培養(yǎng)基
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Exosomal miR-192-5p secreted by bone marrow mesenchymal stem cells inhibits hepatic stellate cell activation and targets PPP2R3A (2023-05-25)
期刊:JOURNAL OF HISTOTECHNOLOGY
DOI:10.1080/01478885.2023.2215151
影響因子 :1.1
引用產(chǎn)品: HSC-T6 細(xì)胞 , 大鼠骨髓間充質(zhì)干細(xì)胞 , DMEM高糖 培養(yǎng)基 , 特級(jí)胎牛血清 , 青霉素-鏈霉素溶液 (雙抗),100× , 大鼠骨髓間充質(zhì)干細(xì)胞完全培養(yǎng)基 , 大鼠骨髓間充質(zhì)干細(xì)胞成骨誘導(dǎo)分化培養(yǎng)基 , 大鼠骨髓間充質(zhì)干細(xì)胞成脂誘導(dǎo)分化培養(yǎng)基
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Titanium dioxide nanotubes increase purinergic receptor P2Y6 expression and activate its downstream PKCα-ERK1/2 pathway in bone marrow mesenchymal stem cells under osteogenic induction (2022-11-26)
期刊:Acta Biomaterialia
DOI:10.1016/j.actbio.2022.11.045
影響因子 :10.6
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞
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3D-printed magnesium-doped wollastonite/nano-hydroxyapatite bioceramic scaffolds with high strength and anti-tumor property (2022-12-06)
期刊:MATERIALS & DESIGN
DOI:10.1016/j.matdes.2022.111464
影響因子 :9.4
引用產(chǎn)品: MG-63 細(xì)胞 , 特級(jí)胎牛血清 , 青霉素-鏈霉素溶液 (雙抗),100× , 大鼠骨髓間充質(zhì)干細(xì)胞
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Research on the osteogenesis and biosafety of ECM–Loaded 3D–Printed Gel/SA/58sBG scaffolds (2022-08-17)
期刊:Frontiers in Bioengineering and Biotechnology
影響因子 :6.1
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞 , 大鼠主動(dòng)脈內(nèi)皮細(xì)胞
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3D printed-electrospun PCL/hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone (2022-12-14)
期刊:Regenerative Biomaterials
影響因子 :5.8
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞
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A novel natural-derived tilapia skin collagen mineralized with hydroxyapatite as a potential bone-grafting scaffold: (2022-03-29)
期刊:Journal Of Biomaterials Applications
影響因子 :2.7
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞
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Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology (2022-05-16)
期刊:Jove-Journal of Visualized Experiments
DOI:10.3791/63971
影響因子 :1.4
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞
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Preparation and In Vitro Characterization of Gelatin Methacrylate for Corneal Tissue Engineering (2021-10-19)
期刊:Tissue Engineering and Regenerative Medicine
DOI:10.1007/s13770-021-00393-6
影響因子 :4.5
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞
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Osteogenic Effect of tsRNA-10277-Loaded Exosome Derived from Bone Mesenchymal Stem Cells on Steroid-Induced Osteonecrosis of the Femoral Head (2020-10-28)
期刊:Drug Design Development and Therapy
影響因子 :4.3
引用產(chǎn)品: 大鼠骨髓間充質(zhì)干細(xì)胞
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