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產(chǎn)品概述
名稱 | K-562 [K562] (人慢性髓原白血病細(xì)胞) (STR鑒定正確) |
別稱 | K562; K 562; GM05372 |
種屬 | 人 |
生長特性 | 懸浮細(xì)胞 |
細(xì)胞形態(tài) | 淋巴細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
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推薦傳代比例 | 3×10^5-5×10^5cells/mL |
推薦換液頻率 | 2-3次/周 |
注意事項 | 該細(xì)胞為懸浮細(xì)胞,請注意離心收集細(xì)胞懸液;請勿直接倒掉細(xì)胞培養(yǎng)液。 |
背景描述 | 連續(xù)細(xì)胞株K-562是由Lozzio從一位臨終的53歲女性慢性骨髓性白血病患者的胸水中建立。K-562細(xì)胞被歸入高度未分化的粒性白細(xì)胞類,Erson等對其表面膜性質(zhì)的研究表明,K-562細(xì)胞是一株人類紅白血病細(xì)胞。K-562細(xì)胞在自然殺傷分析中廣泛作為高敏感的體外受體,See Pross等將K-562細(xì)胞用于NK細(xì)胞的體外詳細(xì)檢測,包括NK細(xì)胞活性的數(shù)學(xué)量化。K-562母細(xì)胞是多能性造血惡性細(xì)胞,它能自發(fā)分化成可識別的紅血球祖細(xì)胞、粒性白細(xì)胞、單核細(xì)胞等。K-562細(xì)胞是EBNA陰性的。 |
年齡(性別) | 女性;53歲 |
組織來源 | 骨髓;慢性骨髓性白血病(CML) |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 白血病細(xì)胞 |
生物安全等級 | BSL-1 |
倍增時間 | ~30-48 hours |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
保藏機(jī)構(gòu) | ATCC; CCL-243 BCRC; 60007 BCRJ; 0126 CCLV; CCLV-RIE 0439 DSMZ; ACC-10 ECACC; 89121407 |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 9,10 D2S1338 17 D3S1358 16 D5S818 11,12 D7S820 9,11 D8S1179 12 D13S317 8 D16S539 11,12 D18S51 15,16 D19S433 14,14.2 D21S11 29,31 FGA 21,24 PentaD 9,13 PentaE 5,14 TH01 9.3 TPOX 8,9 vWA 16 D6S1043 11 D12S391 23 D2S441 10,14 -
STR鑒定圖
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參考文獻(xiàn)
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A Divalent Chikungunya and Zika Nanovaccine with Thermostable Self-assembly Multivalent Scaffold LS-SUMO (2024-02-10)
期刊:Advanced Healthcare Materials
影響因子 :10.0
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , IMDM 培養(yǎng)基
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Pyrotinib and trastuzumab combination treatment synergistically overcomes HER2 dependency in HER2-positive breast cancer: insights from the PHILA trial (2024-10-03)
期刊:EBioMedicine
DOI:10.1016/j.ebiom.2024.105379
影響因子 :9.7
引用產(chǎn)品: SK-BR-3 [SKBR3] 細(xì)胞 , 人外周血單個核細(xì)胞完全培養(yǎng)基 , BT-474 [BT474] 細(xì)胞 , K-562 [K562] 細(xì)胞
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesis (2024-05-17)
期刊:eLife
影響因子 :6.4
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , IMDM 培養(yǎng)基 , 青霉素-鏈霉素溶液(雙抗),100×
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Broadening anticancer spectrum by preprocessing and treatment of T- lymphocytes expressed FcγRI and monoclonal antibodies for refractory cancers (2024-06-17)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2024.1400177
影響因子 :5.7
引用產(chǎn)品: 293T [HEK-293T] 細(xì)胞 , SK-BR-3 [SKBR3] 細(xì)胞 , MCF 10A 細(xì)胞 , MDA-MB-231 細(xì)胞 , Jurkat76 細(xì)胞 , SK-OV-3 細(xì)胞 , K-562 [K562] 細(xì)胞 , MKN-45 細(xì)胞 , Daudi 細(xì)胞 , 人外周血單核細(xì)胞 , SGC-7901 細(xì)胞 , Raji 細(xì)胞 , HCT 116 細(xì)胞
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Cytotoxic and Antibacterial Meroterpenoids Isolated from the Marine-Derived Fungus Talaromyces sp. M27416 (2024-04-20)
期刊:Marine Drugs
影響因子 :5.4
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , TE-1 細(xì)胞 , HOS 細(xì)胞 , GBC-SD 細(xì)胞 , PaTu 8988t 細(xì)胞 , DU 145 細(xì)胞 , 293T [HEK-293T] 細(xì)胞 , SF126 細(xì)胞 , MCF7 [MCF-7] 細(xì)胞 , HCT 116 細(xì)胞 , Hela 細(xì)胞 , A549 [A-549] 細(xì)胞 , Hep G2 細(xì)胞 , A-375 細(xì)胞 , MKN-45 細(xì)胞 , 5637 細(xì)胞 , 786-O [786-0] 細(xì)胞 , CAL-62 細(xì)胞 , A-673 細(xì)胞 , HL-7702 [L-02; LO2] 細(xì)胞
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METTL16 participates in haemoglobin H disease through m6A modification (2024-08-01)
期刊:PLoS One
DOI:10.1371/journal.pone.0306043
影響因子 :2.9
引用產(chǎn)品: K-562 [K562] 細(xì)胞
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Selection of reference genes in liproxstatin-1-treated K562 Leukemia cells via RT-qPCR and RNA sequencing (2024-01-02)
期刊:MOLECULAR BIOLOGY REPORTS
DOI:10.1007/s11033-023-08912-5
影響因子 :2.8
引用產(chǎn)品: K-562 [K562] 細(xì)胞
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AZD8055 Is More Effective Than Rapamycin in Inhibiting Proliferation and Promoting Mitochondrial Clearance in Erythroid Differentiation (2024-10-08)
期刊:Analytical Cellular Pathology
影響因子 :2.6
引用產(chǎn)品: K-562 [K562] 細(xì)胞
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Paclitaxel mediates the PI3K/AKT/mTOR pathway to reduce proliferation of FLT3?ITD+ AML cells and promote apoptosis (2024-02-23)
期刊:Experimental and Therapeutic Medicine
影響因子 :2.4
引用產(chǎn)品: K-562 [K562] 細(xì)胞
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pH-triggered dynamic erosive small molecule chlorambucil nano-prodrugs mediate robust oral chemotherapy (2023-07-25)
期刊:Asian Journal of Pharmaceutical Sciences
DOI:10.1016/j.ajps.2023.100832
影響因子 :10.2
引用產(chǎn)品: Hep G2 細(xì)胞 , H22 細(xì)胞 , MCF7 [MCF-7] 細(xì)胞 , K-562 [K562] 細(xì)胞 , A549 [A-549] 細(xì)胞
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CD71-mediated liposomal arsenic-nickel complex combined with all-trans retinoic acid for the efficacy of acute promyelocytic leukemia (2023-07-07)
期刊:Asian Journal of Pharmaceutical Sciences
DOI:10.1016/j.ajps.2023.100826
影響因子 :10.2
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , THP-1 細(xì)胞
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Label-free, rapid and highly accurate identification and categorization of leukemia cells via Raman spectroscopy (2023-08-22)
期刊:SENSORS AND ACTUATORS B-CHEMICAL
影響因子 :8.4
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , U-937 細(xì)胞 , Raji 細(xì)胞 , Jurkat, Clone E6-1 細(xì)胞 , K-562 [K562]細(xì)胞專用培養(yǎng)基 , U-937細(xì)胞專用培養(yǎng)基 , Raji細(xì)胞專用培養(yǎng)基 , Jurkat, Clone E6-1細(xì)胞專用培養(yǎng)基
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Wedelolactone induces natural killer cell activity and the improvement to bioavailability using polysaccharides from Ligustri Lucidi Fructus (2023-06-05)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2023.125208
影響因子 :8.2
引用產(chǎn)品: NK-92MI 細(xì)胞 , K-562 [K562] 細(xì)胞 , NK-92MI細(xì)胞專用培養(yǎng)基
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Multifunctional targetable liposomal drug delivery system in the management of leukemia: Potential, opportunities, and emerging strategies (2023-05-13)
期刊:LIFE SCIENCES
影響因子 :6.1
引用產(chǎn)品: HL-60 細(xì)胞 , K-562 [K562] 細(xì)胞
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Development and evaluation of novel artemisinin-isatin hybrids with potential anti-leukemic cytotoxicity (2023-04-14)
期刊:Frontiers in Oncology
影響因子 :4.7
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , CCRF-CEM [CCRF CEM] 細(xì)胞
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Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway (2023-12-16)
期刊:Heliyon
DOI:10.1016/j.heliyon.2023.e23580
影響因子 :4.0
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , IMDM 培養(yǎng)基 , 青霉素-鏈霉素溶液(雙抗),100× , 特級胎牛血清
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Ester tethered artemisinin-isatin hybrids: design, synthesis and anti-leukemic activity evaluation (2023-08-21)
期刊:MEDICINAL CHEMISTRY RESEARCH
DOI:10.1007/s00044-023-03122-x
影響因子 :2.6
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , K562/ADR 細(xì)胞 , CCRF-CEM [CCRF CEM] 細(xì)胞 , 人外周血單核細(xì)胞
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Pentanoate-Tethered Artemisinin-Isatin Hybrids with Antileukemic Potential (2023-03-21)
期刊:ChemistrySelect
影響因子 :2.1
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , K562/ADR 細(xì)胞 , CCRF-CEM [CCRF CEM] 細(xì)胞
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Tet2-mediated DNA demethylation regulates the proliferation and apoptosis of human leukemia K562 cells (2023-03-31)
期刊:CELLULAR AND MOLECULAR BIOLOGY
影響因子 :1.6
引用產(chǎn)品: K-562 [K562] 細(xì)胞
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Liproxstatin?1 induces cell cycle arrest, apoptosis, and caspase?3/GSDME?dependent secondary pyroptosis in K562 cells (2022-08-17)
期刊:INTERNATIONAL JOURNAL OF ONCOLOGY
影響因子 :5.9
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , SH-SY5Y [SHSY-5Y] 細(xì)胞
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Antitumor Activity and Mechanism of Action of the Antimicrobial Peptide AMP-17 on Human Leukemia K562 Cells (2022-11-21)
期刊:MOLECULES
影響因子 :4.9
引用產(chǎn)品: K-562 [K562] 細(xì)胞
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Lipid droplets as endogenous intracellular microlenses (2021-12-06)
期刊:Light-Science & Applications
DOI:10.1038/s41377-021-00687-3
影響因子 :19.4
引用產(chǎn)品: K-562 [K562] 細(xì)胞
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CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T?cells (2021-07-16)
期刊:Molecular Therapy-Oncolytics
DOI:10.1016/j.omto.2021.07.003
影響因子 :5.7
引用產(chǎn)品: A549/DDP 細(xì)胞 , COS-1 細(xì)胞 , K-562 [K562] 細(xì)胞 , NCI-H929 細(xì)胞
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Detection of K562 Leukemia Cells in Different States Using a Graphene-SERS Platform (2021-09-15)
期刊:ACS Applied Nano Materials
影響因子 :5.1
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , K-562 [K562]細(xì)胞專用培養(yǎng)基
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Human m6A-mRNA and lncRNA epitranscriptomic microarray reveal function of RNA methylation in hemoglobin H-constant spring disease (2021-10-14)
期刊:Scientific Reports
DOI:10.1038/s41598-021-99867-9
影響因子 :4.6
引用產(chǎn)品: K-562 [K562] 細(xì)胞
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A novel upregulated LncRNA-AC026150.8 promotes chemo-resistance and predicts poor prognosis in acute myeloid leukemia (2021-10-19)
期刊:Cancer Medicine
影響因子 :4.0
引用產(chǎn)品: KG-1 細(xì)胞 , K-562 [K562] 細(xì)胞
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TXNRD1: A Key Regulator Involved in the Ferroptosis of CML Cells Induced by Cysteine Depletion In Vitro (2021-12-07)
期刊:Oxidative Medicine and Cellular Longevity
影響因子 :0.0
引用產(chǎn)品: K-562 [K562] 細(xì)胞 , K-562 [K562] 細(xì)胞
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All-optical targeted drug delivery and real-time detection of a single cancer cell (2020-03-01)
期刊:Nanophotonics
影響因子 :7.5
引用產(chǎn)品: K-562 [K562] 細(xì)胞
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Rational Engineering a Multichannel Upconversion Sensorfor Multiplex Detection of Matrix Metalloproteinases Activities (2018-07-17)
期刊:ACS Sensors
DOI:10.1021/acssensors.8b00320
影響因子 :8.9
引用產(chǎn)品: HT-1080 [HT1080] 細(xì)胞 , K-562 [K562] 細(xì)胞
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