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產(chǎn)品概述
| 名稱 | HCT-15 (人結(jié)直腸腺癌細(xì)胞) (STR鑒定正確) |
| 別稱 | HCT-15; HCT15 |
| 種屬 | 人 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:8 |
| 推薦換液頻率 | 2-3次/周 |
| 背景描述 | DNA指紋鑒定證據(jù)表明，HCT-15細(xì)胞和DLD-1細(xì)胞來源于同一個(gè)人；但同工酶及細(xì)胞染色體組型分析仍存疑問。HCT-15細(xì)胞呈CSAp陰性(CSAp-)；HCT-15角蛋白免疫過氧化物酶染色陽性。 |
| 年齡（性別） | 男性 |
| 組織來源 | 結(jié)直腸腺癌 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 腸癌細(xì)胞 |
| 生物安全等級(jí) | BSL-1 |
| 倍增時(shí)間 | ~20-25 hours |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 基因表達(dá)情況 | carcinoembryonic antigen (CEA) 5.4 ng/10^6 cells/10 days. The cells are positive for keratin by immunoperoxidase staining. |
| 保藏機(jī)構(gòu) | ATCC; CCL-225 BCRC; 60539 DSMZ; ACC-357 ECACC; 91030712 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X,Y CSF1PO 12 D2S1338 17,25 D3S1358 17 D5S818 13 D7S820 10,12 D8S1179 15 D13S317 8,11 D16S539 12,13 D18S51 11,17 D19S433 14,16 D21S11 29,32.2 FGA 22 PentaD 9,14 PentaE 7,14 TH01 7,9.3 TPOX 8,11 vWA 18,19 D6S1043 11,13 D12S391 19,22 D2S441 13,15 -
STR鑒定圖
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參考文獻(xiàn)
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D3S-001, a KRAS G12C inhibitor with rapid target engagement kinetics, overcomes nucleotide cycling and demonstrates robust preclinical and clinical activities (2024-05-08)
期刊：Cancer Discovery
DOI：10.1158/2159-8290.CD-24-0006
影響因子 ：28.2
引用產(chǎn)品： AGS 細(xì)胞 , A549 [A-549] 細(xì)胞 , A-375 細(xì)胞 , KYSE-410 細(xì)胞 , NCI-H358 細(xì)胞 , AsPC-1 細(xì)胞 , BxPC-3 細(xì)胞 , HCT-15 細(xì)胞 , HCT 116 細(xì)胞 , SW480 [SW-480] 細(xì)胞 , SW620 細(xì)胞
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Design, Synthesis, and Antitumor Activity Evaluation of Novel VISTA Small Molecule Inhibitors (2024-02-27)
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.3c02039
影響因子 ：7.3
引用產(chǎn)品： SK-OV-3 細(xì)胞 , HCT-15 細(xì)胞 , CHO 細(xì)胞
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Discovery of Novel 4-Hydroxyquinazoline Derivatives: In Silico, In Vivo and In Vitro Studies Using Primary PARPi-Resistant Cell Lines (2024-03-21)
期刊：MOLECULES
影響因子 ：4.6
引用產(chǎn)品： HCT-15 細(xì)胞 , HCC1937 細(xì)胞 , MDA-MB-231 細(xì)胞 , HL-7702 [L-02; LO2] 細(xì)胞 , NCM460 細(xì)胞
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Long non-coding RNA NEAT1 induced by BHLHE40 activates Wnt/β-catenin signaling and potentiates colorectal cancer progression (2024-08-04)
期刊：Cell Division
DOI：10.1186/s13008-024-00129-7
影響因子 ：2.8
引用產(chǎn)品： HCT-15 細(xì)胞 , LoVo 細(xì)胞 , Caco-2 細(xì)胞
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E3 Ubiquitination Ligase MYLIP Mediates the NKRF/SLC25A34 Axis to Suppress Malignant Progression in Colorectal Cancer (2024-12-11)
期刊：DIGESTIVE DISEASES AND SCIENCES
DOI：10.1007/s10620-024-08735-9
影響因子 ：2.5
引用產(chǎn)品： HCT-15 細(xì)胞 , HCT 116 細(xì)胞 , HT-29 細(xì)胞 , SW480 [SW-480] 細(xì)胞
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Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription (2022-02-18)
期刊：Journal Of Experimental & Clinical Cancer Research
DOI：10.1186/s13046-022-02260-1
影響因子 ：11.3
引用產(chǎn)品： Hs 578T 細(xì)胞 , BT-549 細(xì)胞 , HCT-15 細(xì)胞 , MCF 10A 細(xì)胞 , A549 [A-549] 細(xì)胞 , SK-OV-3 細(xì)胞 , Hela 細(xì)胞 , PANC-1 細(xì)胞
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LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer. (2022-08-23)
期刊：Frontiers in Oncology
影響因子 ：5.7
引用產(chǎn)品： RKO 細(xì)胞 , HT-29 細(xì)胞 , FHC 細(xì)胞 , HCT 116 細(xì)胞 , HCT-15 細(xì)胞 , SW620 細(xì)胞 , SW480 [SW-480] 細(xì)胞
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hsa_circ_0001955 Promotes Colorectal Cancer Progression by Regulating miR-583/FGF21 Axis (2022-05-11)
期刊：Journal of Oncology
影響因子 ：4.5
引用產(chǎn)品： Caco-2 細(xì)胞 , HT-29 細(xì)胞 , HCT-15 細(xì)胞 , HCT 116 細(xì)胞 , SW620 細(xì)胞 , FHC 細(xì)胞
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Identification of KIF21B as a Biomarker for Colorectal Cancer and Associated with Poor Prognosis (2022-11-21)
期刊：Journal of Oncology
影響因子 ：4.5
引用產(chǎn)品： NCM460 細(xì)胞 , HT-29 細(xì)胞 , HCT 116 細(xì)胞 , SW48 細(xì)胞 , HCT-15 細(xì)胞
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CircFAM120B Blocks the Development of Colorectal Cancer by Activating TGF-Beta Receptor II Expression via Targeting miR-645 (2021-07-26)
期刊：Frontiers in Cell and Developmental Biology
影響因子 ：5.5
引用產(chǎn)品： LoVo 細(xì)胞 , HCT-15 細(xì)胞 , RPMI-1640 (含雙抗) 培養(yǎng)基
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LncRNA ENSG00000254615 Modulates Proliferation and 5-FU Resistance by Regulating p21 and Cyclin D1 in Colorectal Cancer (2021-05-01)
期刊：Cancer Investigation
DOI：10.1080/07357907.2021.1923727
影響因子 ：2.4
引用產(chǎn)品： HCT-15 細(xì)胞
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