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產品概述
名稱 | NCI-H358 (人非小細胞肺癌細胞) (STR鑒定正確) |
別稱 | H358; H-358; NCIH358 |
種屬 | 人 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 上皮細胞樣 |
凍存條件 | 凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:4 |
推薦換液頻率 | 2-3次/周 |
背景描述 | NCI-H358細胞是于1981年從一位開始化療之前的患者的腫瘤組織中分離得到的。超微結構研究表明,NCI-H358細胞的細胞質中有Clara細胞的特征結構,NCI-H358細胞表達主要的肺表面結合蛋白SP-A和RNA,不表達SP-B和SP-C;NCI-H358細胞在軟瓊脂中的克隆形成效率為0.83%。 |
年齡(性別) | 男性 |
組織來源 | 細支氣管及肺泡癌;非小細胞肺癌;肺;細支氣管;肺泡 |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 肺癌細胞 |
生物安全等級 | BSL-1 |
倍增時間 | ~38-60 hours |
致瘤性 | Yes, the cells produce tumors in athymic nude mice. |
基因表達情況 | lung surfactant associated protein A (SP-A), The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. |
保藏機構 | ATCC; CRL-5807 ECACC; 95111733 |
STR鑒定
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STR位點信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 17,23 D3S1358 14,18 D5S818 10,12 D7S820 10,11 D8S1179 13,14 D13S317 8,12 D16S539 12,13 D18S51 14 D19S433 13,14 D21S11 28,30 FGA 20,21 PentaD 10,13 PentaE 18 TH01 6 TPOX 8,9 vWA 17 D6S1043 14 D12S391 20 D2S441 11,15 -
STR鑒定圖
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參考文獻
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Discovery of the novel celastrol-based PROTACs for the treatment of non-small cell lung cancer (2025-02-18)
期刊:MOLECULAR DIVERSITY
DOI:10.1007/s11030-025-11140-7
影響因子 :3.9
引用產品: Annexin V-FITC/PI熒光雙染細胞凋亡檢測試劑盒 , NCI-H358 細胞 , NCI-H1975 細胞 , SW620 細胞 , MCF7 [MCF-7] 細胞 , HBZY-1 細胞
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The N6-methyladenosine Epitranscriptomic Landscape of Lung Adenocarcinoma (2024-06-25)
期刊:Cancer Discovery
DOI:10.1158/2159-8290.CD-23-1212
影響因子 :29.7
引用產品: A549 [A-549] 細胞 , NCI-H358 細胞
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D3S-001, a KRAS G12C inhibitor with rapid target engagement kinetics, overcomes nucleotide cycling and demonstrates robust preclinical and clinical activities (2024-05-08)
期刊:Cancer Discovery
DOI:10.1158/2159-8290.CD-24-0006
影響因子 :28.2
引用產品: AGS 細胞 , A549 [A-549] 細胞 , A-375 細胞 , KYSE-410 細胞 , NCI-H358 細胞 , AsPC-1 細胞 , BxPC-3 細胞 , HCT-15 細胞 , HCT 116 細胞 , SW480 [SW-480] 細胞 , SW620 細胞
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NOP2 facilitates EZH2-mediated epithelial–mesenchymal transition by enhancing EZH2 mRNA stability via m5C methylation in lung cancer progression (2024-07-16)
期刊:Cell Death & Disease
DOI:10.1038/s41419-024-06899-w
影響因子 :8.1
引用產品: A549 [A-549] 細胞 , NCI-H358 細胞 , NCI-H1650 細胞 , NCI-H1299 細胞
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USP7 deubiquitinates KRAS and promotes non-small cell lung cancer (2024-11-04)
期刊:Cell Reports
DOI:10.1016/j.celrep.2024.114917
影響因子 :7.5
引用產品: NCI-H1299 細胞 , NCI-H358 細胞 , NCI-H1395 細胞
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Highly consistency of PIK3CA mutation spectrum between circulating tumor DNA and paired tissue in lung cancer patients (2024-07-04)
期刊:Heliyon
DOI:10.1016/j.heliyon.2024.e34013
影響因子 :3.4
引用產品: NCI-H358 細胞 , A549 [A-549] 細胞 , NCI-H1975 細胞 , NCI-H2228 [H2228; H-2228] 細胞 , H3255 細胞 , 特級胎牛血清 , DMEM高糖 培養(yǎng)基
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Combined treatment with cetuximab and STA9090 has synergistic anticancer effects on human non-small cell lung cancer: Combined treatment with cetuximab and STA9090 in NSCLC (2024-05-30)
期刊:ACTA BIOCHIMICA ET BIOPHYSICA SINICA
影響因子 :3.3
引用產品: PC-9 細胞 , A549 [A-549] 細胞 , HCC827 細胞 , NCI-H358 細胞 , NCI-H1299 細胞 , BEAS-2B 細胞
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VT204: A Potential Small Molecule Inhibitor Targeting KRASG12C Mutation for Therapeutic Intervention in Non-Small Cell Lung Cancer (2024-07-25)
期刊:TECHNOLOGY IN CANCER RESEARCH & TREATMENT
影響因子 :2.7
引用產品: NCI-H358 細胞 , A549 [A-549] 細胞
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Design, synthesis, activity and molecular dynamics studies of 1,3,4-thiadiazole derivatives as selective allosteric inhibitors of SHP2 for the treatment of cancer (2023-06-24)
期刊:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1016/j.ejmech.2023.115585
影響因子 :6.7
引用產品: MV-4-11 細胞 , NCI-H358 細胞 , A549 [A-549] 細胞 , MDA-MB-468 細胞
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Non-small cell lung cancer cells with uncommon EGFR exon 19delins variants respond poorly to third-generation EGFR inhibitors (2023-11-24)
期刊:Translational Oncology
DOI:10.1016/j.tranon.2023.101834
影響因子 :5.0
引用產品: NCI-H358 細胞 , A549 [A-549] 細胞 , NCI-H1299 細胞
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Upregulation of NDUFAF2 in Lung Adenocarcinoma Is a Novel Independent Prognostic Biomarker (2023-01-17)
期刊:Computational and Mathematical Methods in Medicine
影響因子 :2.8
引用產品: PC-9 細胞 , NCI-H358 細胞 , A549 [A-549] 細胞 , HCC827 細胞 , NCI-H1299 細胞 , NCI-H1975 細胞 , BEAS-2B 細胞
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A subset of VEGFR‐TKIs activates AMPK in LKB1‐mutant lung cancer (2022-12-18)
期刊:CANCER SCIENCE
影響因子 :6.5
引用產品: A549 [A-549] 細胞 , Calu-1 細胞 , NCI-H358 細胞 , Calu-6 細胞 , NCI-H157 細胞 , NCI-H460 [H460] 細胞
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Exosome-transmitted S100A4 induces immunosuppression and non-small cell lung cancer development by activating STAT3 (2022-11-12)
期刊:CLINICAL AND EXPERIMENTAL IMMUNOLOGY
影響因子 :5.7
引用產品: MRC-5 細胞 , A549 [A-549] 細胞 , NCI-H358 細胞 , NCI-H1299 細胞 , NCI-H460 [H460] 細胞
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YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression (2021-05-31)
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2021.110049
影響因子 :4.8
引用產品: BEAS-2B , HCC827 細胞 , NCI-H1650 細胞 , NCI-H1299 細胞 , NCI-H358 細胞 , A549 [A-549] 細胞 , RPMI-1640 培養(yǎng)基 , 人支氣管上皮細胞完全培養(yǎng)基
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LncRNA CCAT2 promotes tumorigenesis by over-expressed Pokemon in non-small cell lung cancer (2017-01-12)
期刊:Biomedicine & Pharmacotherapy
DOI:10.1016/j.biopha.2016.12.122
影響因子 :7.5
引用產品: PC-9 細胞 , NCI-H358 細胞 , NCI-H1975 細胞 , HBE135-E6E7 細胞
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