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產(chǎn)品概述
名稱 | NCI-H1650 (人非小細(xì)胞肺癌細(xì)胞) (STR鑒定正確) |
別稱 | H1650; H-1650; H1650_CO; NCIH1650 |
種屬 | 人 |
生長(zhǎng)特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:6 |
推薦換液頻率 | 2-3次/周 |
背景描述 | NCI-H1650細(xì)胞是于1987年從一名27歲白人男性(10年煙齡)支氣管肺泡癌患者的胸腔積液中分離得到的。 |
年齡(性別) | 男性;27歲 |
組織來源 | 肺;腺癌;細(xì)支氣管及肺泡癌;轉(zhuǎn)移灶:胸水 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 肺癌細(xì)胞 |
生物安全等級(jí) | BSL-1 |
倍增時(shí)間 | ~42 hours |
保藏機(jī)構(gòu) | ATCC; CRL-5883 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 11 D2S1338 19 D3S1358 18 D5S818 11 D7S820 8,9 D8S1179 12 D13S317 11 D16S539 11,12 D18S51 10 D19S433 15 D21S11 30 FGA 20 PentaD 8 PentaE 12 TH01 9.3 TPOX 11 vWA 18 D6S1043 13 D12S391 22 D2S441 10 -
STR鑒定圖
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參考文獻(xiàn)
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Super-Resolution Fluorescence Imaging Reveals the Mechanism of NRP1 Clustering on Non-Small-Cell Lung Cancer Membranes (2025-01-22)
期刊:ANALYTICAL CHEMISTRY
DOI:10.1021/acs.analchem.4c05675
影響因子 :6.7
引用產(chǎn)品: NCI-H1650 細(xì)胞 , NCI-H1975 細(xì)胞 , HCC827 細(xì)胞
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FAM64A regulates the malignant phenotype and tumor microenvironment of non-small cell lung cancer by activating the JAK/STAT3/PDL1 axis (2025-02-24)
期刊:JOURNAL OF MOLECULAR HISTOLOGY
DOI:10.1007/s10735-024-10339-6
影響因子 :2.9
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , NCI-H1299 細(xì)胞 , NCI-H460 [H460] 細(xì)胞 , NCI-H1650 細(xì)胞 , BEAS-2B 細(xì)胞 , Ham's F-12K 培養(yǎng)基 , RPMI-1640 培養(yǎng)基 , 特級(jí)胎牛血清 , 青霉素-鏈霉素溶液(雙抗),100×
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Brain Metastasis from EGFR-Mutated Non-Small Cell Lung Cancer: Secretion of IL11 from Astrocytes Up-Regulates PDL1 and Promotes Immune Escape (2024-05-02)
期刊:Advanced Science
影響因子 :15.1
引用產(chǎn)品: LLC [LL/2; LLC1] 細(xì)胞 , NCI-H1650 細(xì)胞
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N6-methyladenosine-modified SRPK1 promotes aerobic glycolysis of lung adenocarcinoma via PKM splicing (2024-08-02)
期刊:CELLULAR & MOLECULAR BIOLOGY LETTERS
DOI:10.1186/s11658-024-00622-5
影響因子 :9.2
引用產(chǎn)品: BEAS-2B 細(xì)胞 , NCI-H226 [H226] 細(xì)胞 , HCC827 細(xì)胞 , NCI-H1299 細(xì)胞 , RPMI-1640 培養(yǎng)基 , A549 [A-549] 細(xì)胞 , PC-9 細(xì)胞 , DMEM高糖 培養(yǎng)基 , 293T [HEK-293T] 細(xì)胞 , NCI-H1650 細(xì)胞 , NCI-H1975 細(xì)胞
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NOP2 facilitates EZH2-mediated epithelial–mesenchymal transition by enhancing EZH2 mRNA stability via m5C methylation in lung cancer progression (2024-07-16)
期刊:Cell Death & Disease
DOI:10.1038/s41419-024-06899-w
影響因子 :8.1
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , NCI-H358 細(xì)胞 , NCI-H1650 細(xì)胞 , NCI-H1299 細(xì)胞
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ORC6 acts as an effective prognostic predictor for non?small cell lung cancer and is closely associated with tumor progression (2024-01-11)
期刊:Oncology Letters
影響因子 :2.9
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , PC-9 細(xì)胞 , NCI-H1299 細(xì)胞 , NCI-H1650 細(xì)胞 , BEAS-2B 細(xì)胞
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Identification of a necroptosis-related gene signature for making clinical predictions of the survival of patients with lung adenocarcinoma (2024-01-08)
期刊:PeerJ
影響因子 :2.7
引用產(chǎn)品: BEAS-2B 細(xì)胞 , A549 [A-549] 細(xì)胞 , NCI-H1650 細(xì)胞
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Curcumin Inhibits the Progression of Non-small Cell Lung Cancer by Regulating DMRT3/SLC7A11 Axis (2024-05-14)
期刊:MOLECULAR BIOTECHNOLOGY
DOI:10.1007/s12033-024-01166-x
影響因子 :2.6
引用產(chǎn)品: 人臍靜脈內(nèi)皮細(xì)胞 , 16HBE 細(xì)胞 , NCI-H1650 細(xì)胞
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USP8-mediated PTK7 promotes PIK3CB-related pathway to accelerate the malignant progression of non-small cell lung cancer (2024-11-17)
期刊:Thoracic Cancer
影響因子 :2.3
引用產(chǎn)品: NCI-H1299 細(xì)胞 , THP-1 細(xì)胞 , HBE 細(xì)胞 , A549 [A-549] 細(xì)胞 , HCC827 細(xì)胞 , NCI-H1650 細(xì)胞
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MECOM: a bioinformatics and experimentally identified marker for the diagnosis and prognosis of lung adenocarcinoma (2024-03-05)
期刊:Biomarkers in Medicine
影響因子 :1.9
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , NCI-H1650 細(xì)胞 , BEAS-2B 細(xì)胞
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circPTP4A2 knockdown suppresses NSCLC progression via regulating proliferation and activating anti-tumor immunity (2024-07-16)
期刊:Journal of Cardiothoracic Surgery
DOI:10.1186/s13019-024-02964-9
影響因子 :1.5
引用產(chǎn)品: BEAS-2B 細(xì)胞 , NCI-H1650 細(xì)胞 , Calu-3 細(xì)胞 , A549 [A-549] 細(xì)胞 , NCI-H1299 細(xì)胞
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Effect of circ_0000009 on lung adenocarcinoma progression by regulating PDZD2 in a ceRNA- and RBP- dependent manner (2023-06-10)
期刊:GENE
DOI:10.1016/j.gene.2023.147555
影響因子 :3.5
引用產(chǎn)品: BEAS-2B 細(xì)胞 , A549 [A-549] 細(xì)胞 , NCI-H1650 細(xì)胞 , NCI-H1299 細(xì)胞
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GIMAP7 inhibits epithelial-mesenchymal transition and glycolysis in lung adenocarcinoma cells via regulating the Smo/AMPK signaling pathway (2023-12-27)
期刊:Thoracic Cancer
影響因子 :2.9
引用產(chǎn)品: NCI-H1650 細(xì)胞 , A549 [A-549] 細(xì)胞 , NCI-H1299 細(xì)胞
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Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma (2023-02-24)
期刊:Thoracic Cancer
影響因子 :2.9
引用產(chǎn)品: MRC-5 細(xì)胞 , MRC-5細(xì)胞專用培養(yǎng)基 , NCI-H1299 細(xì)胞 , NCI-H1975 細(xì)胞 , A549 [A-549] 細(xì)胞 , NCI-H1650 細(xì)胞 , PC-9 細(xì)胞
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RPL11 promotes non-small cell lung cancer cell proliferation by regulating endoplasmic reticulum stress and cell autophagy (2023-03-03)
期刊:BMC Molecular and Cell Biology
DOI:10.1186/s12860-023-00469-2
影響因子 :2.8
引用產(chǎn)品: NCI-H1299 細(xì)胞 , NCI-H1650 細(xì)胞
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N6-methyladenosine-induced SVIL antisense RNA 1 restrains lung adenocarcinoma cell proliferation by destabilizing E2F1 (2022-01-22)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2025697
影響因子 :4.9
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , NCI-H1650 細(xì)胞
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Hsa_circ_0017639 regulates cisplatin resistance and tumor growth via acting as a miR-1296-5p molecular sponge and modulating sine oculis homeobox 1 expression in non-small cell lung cancer (2022-03-28)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2053810
影響因子 :4.9
引用產(chǎn)品: 293T [HEK-293T] 細(xì)胞 , NCI-H460 [H460] 細(xì)胞 , NCI-H1650 細(xì)胞 , A549 [A-549] 細(xì)胞 , NCI-H1299 細(xì)胞 , HBE135-E6E7 細(xì)胞
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Identification of a Novel circ_0010235/miR-1249-3p/HOXA13 Axis in Lung Adenocarcinoma (2022-01-25)
期刊:Biochemical Genetics
DOI:10.1007/s10528-021-10181-5
影響因子 :2.4
引用產(chǎn)品: 16HBE 細(xì)胞 , NCI-H1650 細(xì)胞 , NCI-H1299 細(xì)胞
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Curcumin suppresses the malignancy of non-small cell lung cancer by modulating the circ-PRKCA/miR-384/ITGB1 pathway (2021-03-06)
期刊:Biomedicine & Pharmacotherapy
DOI:10.1016/j.biopha.2021.111439
影響因子 :7.5
引用產(chǎn)品: 16HBE 細(xì)胞 , NCI-H1650 細(xì)胞 , NCI-H1299 細(xì)胞 , NCI-H460 [H460] 細(xì)胞 , A549 [A-549] 細(xì)胞 , 293T [HEK-293T] 細(xì)胞 , RPMI-1640 培養(yǎng)基 , 特級(jí)胎牛血清
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YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression (2021-05-31)
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2021.110049
影響因子 :4.8
引用產(chǎn)品: BEAS-2B , HCC827 細(xì)胞 , NCI-H1650 細(xì)胞 , NCI-H1299 細(xì)胞 , NCI-H358 細(xì)胞 , A549 [A-549] 細(xì)胞 , RPMI-1640 培養(yǎng)基 , 人支氣管上皮細(xì)胞完全培養(yǎng)基
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Comprehensive analysis of an immune infiltrate-related competitive endogenous RNA network reveals potential prognostic biomarkers for non-small cell lung cancer (2021-12-02)
期刊:PLoS One
DOI:10.1371/journal.pone.0260720
影響因子 :3.7
引用產(chǎn)品: 青霉素-鏈霉素溶液 (雙抗),100× , NCI-H1650 細(xì)胞 , NCI-H1975 細(xì)胞 , DMEM高糖 培養(yǎng)基
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Circ-METTL15 contributes to the proliferation, metastasis, immune escape and restrains apoptosis in lung cancer by regulating miR-1299/PDL1 axis (2021-11-19)
期刊:Autoimmunity
DOI:10.1080/08916934.2021.2001801
影響因子 :3.5
引用產(chǎn)品: T84 細(xì)胞 , NCI-H1650 細(xì)胞 , NCI-H1299 細(xì)胞 , A549 [A-549] 細(xì)胞 , RPMI-1640 培養(yǎng)基 , 特級(jí)胎牛血清 , 青霉素-鏈霉素溶液 (雙抗),100×
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Overexpression of Human Aspartyl (Asparaginyl) β-hydroxylase in NSCLC: Its Diagnostic Value by Means of Exosomes of Bronchoalveolar Lavage (2021-11-01)
期刊:Applied Immunohistochemistry & Molecular Morphology
DOI:10.1097/PAI.0000000000000963
影響因子 :1.6
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , NCI-H1299 細(xì)胞 , NCI-H1650 細(xì)胞 , HCC827 細(xì)胞
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One-step determination of deletion mutation based on loop-mediated isothermal amplification (2020-12-19)
期刊:Analytical Biochemistry
影響因子 :2.9
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , NCI-H1650 細(xì)胞
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EGFR mutation status in Tunisian non-small-cell lung cancer patients evaluated by mutation-specific immunohistochemistry (2018-08-09)
期刊:BMC Pulmonary Medicine
影響因子 :3.1
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , NCI-H1650 細(xì)胞 , NCI-H1975 細(xì)胞
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