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Calu-3 (人肺腺癌細(xì)胞(胸水)) (STR鑒定正確)
貨號(hào):CL-0054
規(guī)格:1×10?cells/T25培養(yǎng)瓶
價(jià)格: ¥1600
生長(zhǎng)培養(yǎng)基:MEM(含NEAA)+10% FBS+1% P/S
產(chǎn)品概述
名稱 | Calu-3 (人肺腺癌細(xì)胞(胸水)) (STR鑒定正確) |
別稱 | CaLu-3; CALU-3; Calu 3; Calu3; CALU3 |
種屬 | 人 |
生長(zhǎng)特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
生長(zhǎng)培養(yǎng)基:
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:4 |
推薦換液頻率 | 2-3次/周 |
注意事項(xiàng) | 該細(xì)胞較難消化,注意延長(zhǎng)消化時(shí)間,消化到細(xì)胞收縮變圓,輕拍培養(yǎng)瓶側(cè)邊,細(xì)胞可以滑落方可終止消化。 |
背景描述 | Calu-3細(xì)胞是從一名25歲的白人男性肺腺癌患者的胸水中分離建系的;該患者曾使用過(guò)環(huán)磷酰胺、博來(lái)霉素、阿霉素進(jìn)行治療。Calu-3細(xì)胞接種至裸鼠可成瘤;Calu-3細(xì)胞亦可作轉(zhuǎn)染宿主。 |
年齡(性別) | 男性;25歲 |
組織來(lái)源 | 肺;轉(zhuǎn)移灶;胸水腺癌 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 肺癌細(xì)胞 |
生物安全等級(jí) | BSL-1 |
倍增時(shí)間 | ~35-45 hours |
致瘤性 | Yes, forms well differentiated grade I adenocarcinoma in nude mice. |
抗原表達(dá)情況 | Blood Type A; Rh+ |
基因表達(dá)情況 | Blood Type A; Rh+ |
保藏機(jī)構(gòu) | ATCC; HTB-55 BCRJ; 0264 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 11,12 D2S1338 19 D3S1358 15,18 D5S818 11 D7S820 10,11 D8S1179 11,15 D13S317 12 D16S539 12,14 D18S51 14 ,17 D19S433 12,13 D21S11 28,30 FGA 25 PentaD 9,16 PentaE 5,21 TH01 6,9.3 TPOX 8 vWA 16,17 D6S1043 11 D12S391 21,22 D2S441 14 -
STR鑒定圖
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參考文獻(xiàn)
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Inhalable Chemotactic Liposome for Targeted Modulation of Pulmonary Pre-Metastatic Niche (2025-01-16)
期刊:ADVANCED FUNCTIONAL MATERIALS
影響因子 :18.5
引用產(chǎn)品: Calu-3 細(xì)胞
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Inhalable lipid-based nanocarriers covered by polydopamine for effective mucus penetration and pulmonary retention (2025-02-19)
期刊:COLLOIDS AND SURFACES B-BIOINTERFACES
DOI:10.1016/j.colsurfb.2025.114576
影響因子 :5.4
引用產(chǎn)品: Calu-3 細(xì)胞
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Fc-empowered exosomes with superior epithelial layer transmission and lung distribution ability for pulmonary vaccination (2024-09-12)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2024.08.015
影響因子 :18.0
引用產(chǎn)品: Calu-3 細(xì)胞
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Primate-specific BTN3A2 protects against SARS-CoV-2 infection by interacting with and reducing ACE2 (2024-08-13)
期刊:EBioMedicine
DOI:10.1016/j.ebiom.2024.105281
影響因子 :9.7
引用產(chǎn)品: Calu-3 細(xì)胞
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Targeting TYK2 alleviates Rab27A-induced malignant progression of non-small cell lung cancer via disrupting IFNα-TYK2-STAT-HSPA5 axis (2024-03-23)
期刊:npj Precision Oncology
DOI:10.1038/s41698-024-00574-1
影響因子 :7.9
引用產(chǎn)品: 293T [HEK-293T] 細(xì)胞 , BEAS-2B 細(xì)胞 , NCI-H1299 細(xì)胞 , A549 [A-549] 細(xì)胞 , LTEP-α-2 細(xì)胞 , NCI-H226 [H226] 細(xì)胞 , Calu-3 細(xì)胞 , HCC827 細(xì)胞 , NCI-H1975 細(xì)胞
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Activation of Hypoxia Inducible Factor-1 Alpha-Mediated DNA Methylation Enzymes (DNMT3a and TET2) Under Hypoxic Conditions Regulates S100A6 Transcription to Promote Lung Cancer Cell Growth and Metastasis (2024-03-18)
期刊:ANTIOXIDANTS & REDOX SIGNALING
影響因子 :6.6
引用產(chǎn)品: BEAS-2B 細(xì)胞 , Calu-3 細(xì)胞 , NCI-H1395 細(xì)胞 , NCI-H1975 細(xì)胞 , A549 [A-549] 細(xì)胞
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Synergistic inhibition effects of andrographolide and baicalin on coronavirus mechanisms by downregulation of ACE2 protein level (2024-02-21)
期刊:Scientific Reports
DOI:10.1038/s41598-024-54722-5
影響因子 :4.6
引用產(chǎn)品: Calu-3 細(xì)胞
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HNRNPD/MAD2L2 axis facilitates lung adenocarcinoma progression and is a potential prognostic biomarker (2024-10-02)
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2024.111443
影響因子 :4.4
引用產(chǎn)品: Calu-3 細(xì)胞 , A549 [A-549] 細(xì)胞 , NCI-H1975 細(xì)胞 , PC-9 細(xì)胞 , 人臍靜脈內(nèi)皮細(xì)胞
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DNMT3A-mediated DNA methylation and transcription inhibition of FZD5 suppresses lung carcinogenesis (2024-04-15)
期刊:Heliyon
DOI:10.1016/j.heliyon.2024.e29733
影響因子 :3.4
引用產(chǎn)品: NCI-H1299 細(xì)胞 , Calu-3 細(xì)胞
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PM2.5 Exposure Inhibits Transepithelial Anion Short-circuit Current by Downregulating P2Y2 Receptor/CFTR Pathway (2024-07-22)
期刊:International Journal of Medical Sciences
影響因子 :3.2
引用產(chǎn)品: Calu-3 細(xì)胞
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Knockdown of HM13 Inhibits Metastasis, Proliferation, and M2 Macrophage Polarization of Non-small Cell Lung Cancer Cells by Suppressing the JAK2/STAT3 Signaling Pathway (2024-08-29)
期刊:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
DOI:10.1007/s12010-024-05054-7
影響因子 :3.1
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , NCI-H1975 細(xì)胞 , NCI-H1299 細(xì)胞 , Calu-3 細(xì)胞
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Circular RNA circ_001621 acts as a tumor promoter in lung cancer by regulating the miR-199a-3p/GREM1 axis (2024-06-28)
期刊:Archives of Medical Science
影響因子 :3.0
引用產(chǎn)品: Calu-3 細(xì)胞 , A549 [A-549] 細(xì)胞 , NCI-H1975 細(xì)胞 , PC-9 細(xì)胞 , BEAS-2B 細(xì)胞 , RPMI-1640 培養(yǎng)基 , MEM 培養(yǎng)基 , Ham's F-12K 培養(yǎng)基 , DMEM高糖 培養(yǎng)基 , 特級(jí)胎牛血清
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circPTP4A2 knockdown suppresses NSCLC progression via regulating proliferation and activating anti-tumor immunity (2024-07-16)
期刊:Journal of Cardiothoracic Surgery
DOI:10.1186/s13019-024-02964-9
影響因子 :1.5
引用產(chǎn)品: BEAS-2B 細(xì)胞 , NCI-H1650 細(xì)胞 , Calu-3 細(xì)胞 , A549 [A-549] 細(xì)胞 , NCI-H1299 細(xì)胞
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Aryl hydrocarbon receptor is a proviral host factor and a candidate pan-SARS-CoV-2 therapeutic target (2023-05-31)
期刊:Science Advances
影響因子 :13.6
引用產(chǎn)品: Hep G2 細(xì)胞 , HuH-7 細(xì)胞 , NCI-H1299 細(xì)胞 , BEAS-2B 細(xì)胞 , Calu-3 細(xì)胞
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GPR37-centered ceRNA network contributes to metastatic potential in lung adenocarcinoma: Evidence from high-throughput sequencing (2023-11-16)
期刊:Translational Oncology
DOI:10.1016/j.tranon.2023.101819
影響因子 :5.0
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , NCI-H1395 細(xì)胞 , NCI-H1975 細(xì)胞 , Calu-3 細(xì)胞
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Decreased Sphingosine Due to Down-Regulation of Acid Ceramidase Expression in Airway of Bronchiectasis Patients: A Potential Contributor to Pseudomonas aeruginosa Infection (2023-04-27)
期刊:Infection and Drug Resistance
影響因子 :3.9
引用產(chǎn)品: Calu-3 細(xì)胞
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Hsa_circ_0080608 Attenuates Lung Cancer Progression by Functioning as a Competitive Endogenous RNA to Regulate the miR-661/ADRA1A Pathway (2023-10-11)
期刊:HORMONE AND METABOLIC RESEARCH
影響因子 :2.2
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , Calu-3 細(xì)胞 , NCI-H1975 細(xì)胞 , PC-9 細(xì)胞 , BEAS-2B 細(xì)胞 , RPMI-1640 培養(yǎng)基 , 特級(jí)胎牛血清 , DMEM高糖 培養(yǎng)基
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APOE interacts with ACE2 inhibiting SARS-CoV-2 cellular entry and inflammation in COVID-19 patients (2022-08-01)
期刊:Signal Transduction and Targeted Therapy
DOI:10.1038/s41392-022-01118-4
影響因子 :38.1
引用產(chǎn)品: Calu-3 細(xì)胞
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Characteristics of replication and pathogenicity of SARS-CoV-2 Alpha and Delta isolates (2022-09-24)
期刊:VIROLOGICA SINICA
DOI:10.1016/j.virs.2022.09.007
影響因子 :7.0
引用產(chǎn)品: Calu-3 細(xì)胞
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Comprehensive Analysis of a Novel Immune-Related Gene Signature in Lung Adenocarcinoma (2022-10-19)
期刊:Journal of Clinical Medicine
影響因子 :5.0
引用產(chǎn)品: A549 [A-549] 細(xì)胞 , NCI-H1975 細(xì)胞 , Calu-3 細(xì)胞 , BEAS-2B 細(xì)胞 , MEM (含NEAA) 培養(yǎng)基
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CircRNA ZNF609 Knockdown Represses the Development of Non-Small Cell Lung Cancer via miR-623/FOXM1 Axis (2022-12-21)
期刊:Cancer Management and Research
影響因子 :3.6
引用產(chǎn)品: HBE135-E6E7 細(xì)胞 , Calu-6 細(xì)胞 , A549 [A-549] 細(xì)胞 , Calu-3 細(xì)胞 , NCI-H1299 細(xì)胞
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The HNF4α-BC200-FMR1 positive feedback loop promotes growth and metastasis in invasive mucinous lung adenocarcinoma (2021-01-01)
期刊:Cancer Res?
DOI:10.1158/0008-5472.CAN-21-0980
影響因子 :11.2
引用產(chǎn)品: Calu-3 細(xì)胞
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Downregulation of the Circadian Rhythm Regulator HLF Promotes Multiple-Organ Distant Metastases in Non-Small Cell Lung Cancer Through PPAR NF-κb Signaling (2020-07-10)
期刊:Cancer Letters
DOI:10.1016/j.canlet.2020.04.007
影響因子 :9.7
引用產(chǎn)品: Calu-3 細(xì)胞 , WI-38 細(xì)胞
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