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RKO (人結腸腺癌細胞) (STR鑒定正確)
貨號:CL-0196
規(guī)格:1×10?cells/T25培養(yǎng)瓶
價格: ¥1500
生長培養(yǎng)基:MEM(含NEAA)+10% FBS+1% P/S
產(chǎn)品概述
名稱 | RKO (人結腸腺癌細胞) (STR鑒定正確) |
種屬 | 人 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 上皮細胞樣 |
凍存條件 | 凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
生長培養(yǎng)基:
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:4 |
推薦換液頻率 | 2-3次/周 |
注意事項 | 該細胞對溫度變化敏感,培養(yǎng)時使用的培養(yǎng)基需預熱,溫度較低的培養(yǎng)基使用會導致變圓收縮;傳代后到細胞完全伸展開需要72h以上。 |
背景描述 | RKO細胞是一個低分化的結腸癌細胞系。RKO細胞含有野生型p53,但缺乏人甲狀腺受體核受體(h-TRβ1)。RKO細胞的p53蛋白的水平高于RKO-E6細胞,RKO細胞是RKO-E6細胞和RKO-AS45-1細胞的親本細胞系。RKO細胞在裸鼠中成瘤,且在軟瓊脂中形成集落。 |
組織來源 | 結腸癌 |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 腸癌細胞 |
生物安全等級 | BSL-1 |
倍增時間 | ~21 hours |
致瘤性 | Yes, in nude mice.Yes, in soft agar. |
受體表達情況 | urokinase receptor (u-PAR) |
保藏機構 | ATCC; CRL-2577 |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 8,10 D2S1338 16 D3S1358 16,19 D5S818 11,13 D7S820 8,10 D8S1179 9,13,14 D13S317 8,11 D16S539 12,13 D18S51 11,12 D19S433 14 D21S11 27,30 FGA 20,21,22,23 PentaD 10,11 PentaE 11,13 TH01 6,10 TPOX 11 vWA 16,22 D6S1043 14.1,19 D12S391 15,20 D2S441 11 -
STR鑒定圖
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參考文獻
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Therapy-induced senescent tumor cell-derived extracellular vesicles promote colorectal cancer progression through SERPINE1-mediated NF-κB p65 nuclear translocation (2024-04-04)
期刊:Molecular Cancer
DOI:10.1186/s12943-024-01985-1
影響因子 :37.3
引用產(chǎn)品: 293T [HEK-293T] 細胞 , RKO 細胞 , HCT 116 細胞
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Mitochondrial genome transfer drives metabolic reprogramming in adjacent colonic epithelial cells promoting TGFβ1-mediated tumor progression (2024-04-30)
期刊:Nature Communications
DOI:10.1038/s41467-024-48100-y
影響因子 :16.6
引用產(chǎn)品: SW480 [SW-480] 細胞 , 293T [HEK-293T] 細胞 , HCT 116 細胞 , HT-29 細胞 , SW620 細胞 , RKO 細胞
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Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis (2024-01-17)
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2024.01.006
影響因子 :14.5
引用產(chǎn)品: 特級胎牛血清 , LoVo 細胞 , HT-29 細胞 , HCT 116 細胞 , SW620 細胞 , RKO 細胞 , DLD-1 細胞 , HCT-8 [HRT-18] 細胞
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Enhancing tumor-specific recognition of programmable synthetic bacterial consortium for precision therapy of colorectal cancer (2024-01-20)
期刊:npj Biofilms and Microbiomes
DOI:10.1038/s41522-024-00479-8
影響因子 :9.2
引用產(chǎn)品: CT26.WT 細胞 , RKO 細胞 , SW480 [SW-480] 細胞
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Repurposing cyclovirobuxine D as a novel inhibitor of colorectal cancer progression via modulating the CCT3/YAP axis (2024-07-11)
期刊:BRITISH JOURNAL OF PHARMACOLOGY
影響因子 :6.8
引用產(chǎn)品: RKO 細胞
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A probiotic Limosilactobacillus fermentum GR-3 mitigates colitis-associated tumorigenesis in mice via modulating gut microbiome (2024-09-06)
期刊:npj Science of Food
DOI:10.1038/s41538-024-00307-5
影響因子 :6.3
引用產(chǎn)品: SW480 [SW-480] 細胞 , RKO 細胞
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Antitumor effects of IOX1 combined with bevacizumab-induced apoptosis and immunity on colorectal cancer cells (2024-08-14)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.112896
影響因子 :4.8
引用產(chǎn)品: HCT 116 細胞 , RKO 細胞
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Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1 (2024-04-23)
期刊:ONCOLOGY REPORTS
影響因子 :4.2
引用產(chǎn)品: RKO 細胞 , SW620 細胞 , HCT-8 [HRT-18] 細胞 , HCT 116 細胞 , HT-29 細胞
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10-Secocycloartane (=9,19-cyclo-9,10-secolanostane) triterpenoid saponins: Huangqiyenins M–X from Astragalus membranaceus (Fisch.) Bge (2024-03-30)
期刊:PHYTOCHEMISTRY
DOI:10.1016/j.phytochem.2024.114072
影響因子 :3.8
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TL1A promotes metastasis and EMT process of colorectal cancer (2024-01-15)
期刊:Heliyon
DOI:10.1016/j.heliyon.2024.e24392
影響因子 :3.4
引用產(chǎn)品: SW480 [SW-480] 細胞 , LoVo 細胞 , SW620 細胞 , RKO 細胞 , HCT 116 細胞 , HCT116 細胞(熒光素酶標記) , FHC 細胞
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DDX18 influences chemotherapy sensitivity in colorectal cancer by regulating genomic stability (2024-11-20)
期刊:EXPERIMENTAL CELL RESEARCH
DOI:10.1016/j.yexcr.2024.114344
影響因子 :3.3
引用產(chǎn)品: SW620 細胞 , SW480 [SW-480] 細胞 , RKO 細胞 , HCT 116 細胞 , HT-29 細胞
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Suppression of Tumor or Host Intrinsic CMTM6 Drives Antitumor Cytotoxicity in a PD-L1–Independent Manner (2023-02-03)
期刊:Cancer Immunology Research
DOI:10.1158/2326-6066.CIR-22-0439
影響因子 :10.1
引用產(chǎn)品: Jurkat, Clone E6-1 細胞 , RKO 細胞 , CT26.WT 細胞 , B16-F10 細胞 , MC38 細胞 , 4T1 細胞 , LLC [LL/2; LLC1] 細胞 , Hepa 1-6 細胞 , YAC-1 細胞 , 293T [HEK-293T] 細胞 , AAV-293 細胞 , STR鑒定
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Attenuated Salmonella-delivered PD-1 siRNA enhances the antitumor effects of EZH2 inhibitors in colorectal cancer (2023-09-12)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2023.110918
影響因子 :5.6
引用產(chǎn)品: RKO 細胞 , HCT 116 細胞
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m6A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression (2023-08-29)
期刊:Aging-US
影響因子 :5.2
引用產(chǎn)品: 人結腸黏膜上皮細胞 , RKO 細胞 , HCT 116 細胞 , SW480 [SW-480] 細胞 , COLO 320DM 細胞
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The role of KNTC1 in the regulation of proliferation, migration and tumorigenesis in colorectal cancer (2023-05-23)
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2023.110728
影響因子 :4.8
引用產(chǎn)品: HCT 116 細胞 , RKO 細胞 , SW480 [SW-480] 細胞
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Fluorescent Quinolinium Derivative as Novel Mitochondria Probe and Function Modulator by Targeting Mitochondrial RNA (2023-03-16)
期刊:MOLECULES
影響因子 :4.6
引用產(chǎn)品: RKO 細胞 , 青霉素-鏈霉素溶液 (雙抗),100× , NCM460 細胞
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Screening and validation of the optimal panel of reference genes in colonic epithelium and relative cancer cell lines (2023-10-18)
期刊:Scientific Reports
DOI:10.1038/s41598-023-45174-4
影響因子 :4.6
引用產(chǎn)品: RKO 細胞
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CILP2: A prognostic biomarker associated with immune infiltration in colorectal cancer (2023-04-20)
期刊:Heliyon
DOI:10.1016/j.heliyon.2023.e15535
影響因子 :4.0
引用產(chǎn)品: HCT 116 細胞 , SW480 [SW-480] 細胞 , SW620 細胞 , RKO 細胞 , 人結腸黏膜上皮細胞
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ELAPOR1 suppresses tumor progression in colorectal cancer and indicates favorable prognosis (2023-06-03)
期刊:Cancer Biomarkers
影響因子 :3.1
引用產(chǎn)品: SW620 細胞 , RKO 細胞 , SW480 [SW-480] 細胞 , HT-29 細胞 , Caco-2 細胞 , HCT 116 細胞 , 293T [HEK-293T] 細胞
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TRIM29 knockdown prevented the colon cancer progression through decreasing the ubiquitination levels of KRT5 (2023-08-30)
期刊:Open Life Sciences
影響因子 :2.2
引用產(chǎn)品: 人結腸黏膜上皮細胞 , SW480 [SW-480] 細胞 , HCT 116 細胞 , SW620 細胞 , LoVo 細胞 , RKO 細胞
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Exosomes-derived miR-548am-5p promotes colorectal cancer progression (2023-11-30)
期刊:CELLULAR AND MOLECULAR BIOLOGY
DOI:10.14715/cmb/2023.69.12.17
影響因子 :1.6
引用產(chǎn)品: HCT 116 細胞 , SW480 [SW-480] 細胞 , RKO 細胞 , LoVo 細胞
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Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA (2022-12-15)
期刊:JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI:10.1186/s13046-022-02551-7
影響因子 :12.7
引用產(chǎn)品: 293T [HEK-293T] 細胞 , NCM460 細胞 , SW480 [SW-480] 細胞 , DLD-1 細胞 , HT-29 細胞 , RKO 細胞 , HCT 116 細胞
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LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer. (2022-08-23)
期刊:Frontiers in Oncology
影響因子 :5.7
引用產(chǎn)品: RKO 細胞 , HT-29 細胞 , FHC 細胞 , HCT 116 細胞 , HCT-15 細胞 , SW620 細胞 , SW480 [SW-480] 細胞
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Three New Compounds, Licopyranol A–C, Together with Eighteen Known Compounds Isolated from Glycyrrhiza glabra L. and Their Antitumor Activities (2022-09-23)
期刊:Metabolites
影響因子 :5.6
引用產(chǎn)品: RKO 細胞
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Variation of Saponins in Sanguisorba officinalis L. before and after Processing (Paozhi) and Its Effects on Colon Cancer Cells In Vitro (2022-12-19)
期刊:MOLECULES
影響因子 :4.9
引用產(chǎn)品: HCT 116 細胞 , RKO 細胞
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EGCG Enhances the Chemosensitivity of Colorectal Cancer to Irinotecan through GRP78-MediatedEndoplasmic Reticulum Stress (2022-09-13)
期刊:Journal of Oncology
影響因子 :4.5
引用產(chǎn)品: RKO 細胞
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Methyltransferase like 3 enhances pinin mRNA stability through N6-methyladenosine modification to augment tumourigenesis of colon adenocarcinoma (2022-08-22)
期刊:EXPERIMENTAL PHYSIOLOGY
DOI:10.1113/EP090273
影響因子 :2.9
引用產(chǎn)品: RKO 細胞 , LS 174T [LS174T] 細胞 , CW-2 細胞 , SW1116 細胞 , 人結腸黏膜上皮細胞 , 人結腸黏膜上皮細胞完全培養(yǎng)基
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miR?146a?5p negatively regulates the IL?1β?stimulated inflammatory response via downregulation of the IRAK1/TRAF6 signaling pathway in human intestinal epithelial cells (2022-08-05)
期刊:Experimental and Therapeutic Medicine
影響因子 :2.8
引用產(chǎn)品: RKO 細胞
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PD-1-siRNA Delivered by Attenuated Salmonella Enhances the Antitumor Effect of Chloroquine in Colon Cancer (2021-07-06)
期刊:Frontiers in Immunology
影響因子 :7.3
引用產(chǎn)品: HCT 116 細胞 , RKO 細胞
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Notum palmitoleoyl-protein carboxylesterase regulates Fas cell surface death receptor-mediated apoptosis via the Wnt signaling pathway in colon adenocarcinoma (2021-08-17)
期刊:Bioengineered
DOI:10.1080/21655979.2021.1961657
影響因子 :4.9
引用產(chǎn)品: RKO 細胞 , HCT 116 細胞 , SW620 細胞
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Lycorine Induces autophagy-associated apoptosis by targeting MEK2 and enhances vemurafenib activity in colorectal cancer (2020-01-03)
期刊:Aging-US
影響因子 :5.2
引用產(chǎn)品: HCT 116 細胞 , SW480 [SW-480] 細胞 , RKO 細胞 , CT26.WT 細胞
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The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway (2020-09-26)
期刊:Investigational New Drugs
DOI:10.1007/s10637-020-01014-0
影響因子 :3.4
引用產(chǎn)品: HCT 116 細胞 , SW480 [SW-480] 細胞 , SW620 細胞 , HT-29 細胞 , LoVo 細胞 , RKO 細胞
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Pseudophosphatase STYX promotes tumor growth and metastasis by inhibiting FBXW7 function in colorectal cancer (2019-06-10)
期刊:Cancer Letters
DOI:10.1016/j.canlet.2019.04.014
影響因子 :9.7
引用產(chǎn)品: SW480 [SW-480] 細胞 , SW620 細胞 , HCT 116 細胞 , HT-29 細胞 , RKO 細胞 , LoVo 細胞
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