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NK-92 (人惡性非霍奇金淋巴瘤患者的自然殺傷細(xì)胞) (STR鑒定正確)
貨號(hào):CL-0530
規(guī)格:1×10?cells/T25培養(yǎng)瓶
價(jià)格: ¥2200
生長培養(yǎng)基:MEMα+0.2mM Inositol+0.1mM β-mercaptoethanol+0.02mM Folic Acid+100-200U/mL recombinant IL-2+12.5% HS+12.5% FBS+1% P/S
產(chǎn)品概述
名稱 | NK-92 (人惡性非霍奇金淋巴瘤患者的自然殺傷細(xì)胞) (STR鑒定正確) |
別稱 | NK92; Natural Killer-92; NK-92.05; Neukoplast; aNK |
種屬 | 人類 |
生長特性 | 懸浮細(xì)胞 |
細(xì)胞形態(tài) | 淋巴母細(xì)胞樣 |
凍存條件 | 凍存液:90%FBS+10%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
生長培養(yǎng)基:
MEMα+0.2mM Inositol+0.1mM β-mercaptoethanol+0.02mM Folic Acid+100-200U/mL recombinant IL-2+12.5% HS+12.5% FBS+1% P/S
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 5×10^5-1×10^6cells/mL |
推薦換液頻率 | 2-3次/周 |
注意事項(xiàng) | 該細(xì)胞為懸浮細(xì)胞,請(qǐng)注意離心收集細(xì)胞懸液;請(qǐng)勿直接倒掉細(xì)胞培養(yǎng)液。 |
背景描述 | NK-92細(xì)胞是從一位患有急進(jìn)性非霍奇金淋巴瘤的50歲白人男性外周血單核細(xì)胞衍生來的一株IL-2依賴型NK細(xì)胞株。NK-92細(xì)胞對(duì)很多惡性細(xì)胞有細(xì)胞毒性;鉻釋放試驗(yàn)顯示它能殺死K562細(xì)胞和Daudi細(xì)胞。NK-92細(xì)胞(經(jīng)過照射以防止增殖)可以有效地用于血液中白血病的體外免疫清掃而不危及血細(xì)胞的功能。NK-92細(xì)胞有以下特征:CD2、CD7、CD11a、CD28、CD45、CD54表面標(biāo)記陽性;CD1、CD3、CD4、CD5、CD8、CD10、CD14、CD16、CD19、CD20、CD23、CD34和HLA-DR表面標(biāo)記陰性。 |
年齡(性別) | 男性;50歲 |
組織來源 | 外周血 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 淋巴瘤細(xì)胞 |
生物安全等級(jí) | BSL-2 |
倍增時(shí)間 | ~40-50 hours |
保藏機(jī)構(gòu) | ATCC; CRL-2407 DSMZ; ACC-488 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 19,20 D3S1358 15 D5S818 12,13 D7S820 10,11 D8S1179 12 D13S317 9,12 D16S539 11,12 D18S51 12,17 D19S433 14,15 D21S11 31.2,32 FGA 20,22 PentaD 10,12 PentaE 12 TH01 6,9.3 TPOX 8 vWA 18 D6S1043 11,12 D12S391 19 D2S441 14 -
STR鑒定圖
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參考文獻(xiàn)
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AKR1D1 suppresses liver cancer progression by promoting bile acid metabolism-mediated NK cell cytotoxicity (2025-02-25)
期刊:Cell Metabolism
DOI:10.1016/j.cmet.2025.01.011
影響因子 :27.7
引用產(chǎn)品: NK-92 細(xì)胞
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Non-destructive in situ monitoring of structural changes of 3D tumor spheroids during the formation, migration, and fusion process (2025-02-12)
期刊:eLife
影響因子 :6.4
引用產(chǎn)品: DU 145 細(xì)胞 , NK-92 細(xì)胞 , NK-92細(xì)胞專用培養(yǎng)基
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Strategic and Technical Considerations in Manufacturing Viral Vector Vaccines for the Biomedical Advanced Research and Development Authority Threats (2025-01-14)
期刊:Vaccines
影響因子 :5.2
引用產(chǎn)品: NK-92 細(xì)胞
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IL17A/F secreted by ASCT2-overexpression ovarian cancer cells contributes to immune escape through the suppression of natural killer (NK) cells cytotoxicity by the activation of c-JUN/ PTGS2 pathway (2025-02-14)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2025.114226
影響因子 :4.8
引用產(chǎn)品: NK-92 細(xì)胞 , MEMα 培養(yǎng)基 , 人源NK細(xì)胞
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APOC1 inhibit NKTCL doxorubicin sensitivity by promoting mitophagy (2025-01-16)
期刊:IUBMB LIFE
DOI:10.1002/iub.2942
影響因子 :3.7
引用產(chǎn)品: NK-92 細(xì)胞 , NK-92細(xì)胞專用培養(yǎng)基
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The role of MUC16 in tumor biology and tumor immunology in ovarian cancer (2025-02-19)
期刊:BMC CANCER
DOI:10.1186/s12885-025-13461-0
影響因子 :3.4
引用產(chǎn)品: NK-92 細(xì)胞
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Exosomal miR-552-5p Regulates the Role of NK Cells in EMT of Gastric Cancer via the PD-1/PD-L1 Axis (2025-01-01)
期刊:Journal of Cancer
影響因子 :3.3
引用產(chǎn)品: NK-92 細(xì)胞
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2D4, a humanized monoclonal antibody targeting CD132, is a promising treatment for systemic lupus erythematosus (2024-11-17)
期刊:Signal Transduction and Targeted Therapy
DOI:10.1038/s41392-024-02017-6
影響因子 :40.8
引用產(chǎn)品: 馬血清 , NK-92 細(xì)胞
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Targeting Caveolin-1 in Multiple Myeloma Cells Enhances Chemotherapy and Natural Killer Cell-Mediated Immunotherapy (2024-12-04)
期刊:Advanced Science
影響因子 :14.3
引用產(chǎn)品: NK-92 細(xì)胞 , MEMα(不含核苷) 培養(yǎng)基 , 特級(jí)胎牛血清 , 馬血清
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Off-the-shelf CAR-NK cells targeting immunogenic cell death marker ERp57 execute robust antitumor activity and have a synergistic effect with ICD inducer oxaliplatin (2024-07-01)
期刊:Journal for ImmunoTherapy of Cancer
影響因子 :10.3
引用產(chǎn)品: NK-92 細(xì)胞 , MKN-45 細(xì)胞 , Eca-109 細(xì)胞 , IM95 細(xì)胞 , 293T/17 細(xì)胞 , MOLM-13 細(xì)胞
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Multi-omics revealed that ELAVL3 regulates MYCN in neuroblastoma via immunogenic cell death: Risk stratification and experimental research (2024-10-31)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.137045
影響因子 :7.7
引用產(chǎn)品: NK-92細(xì)胞專用培養(yǎng)基 , NK-92 細(xì)胞
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NK92 cells and peripheral blood NK cells respond oppositely upon dasatinib treatment (2024-02-15)
期刊:IMMUNOLOGY
影響因子 :6.4
引用產(chǎn)品: 青霉素-鏈霉素溶液 (雙抗),100× , NK-92 細(xì)胞 , MEMα (不含核苷) 培養(yǎng)基 , IL-2 (重組人白細(xì)胞介素-2、白細(xì)胞介素-2) (250μL×4) , 馬血清 , 特級(jí)胎牛血清
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Assessing immune hepatotoxicity of troglitazone with a versatile liver-immune-microphysiological-system (2024-05-30)
期刊:Frontiers in Pharmacology
DOI:10.3389/fphar.2024.1335836
影響因子 :5.6
引用產(chǎn)品: HL-60 細(xì)胞 , NK-92 細(xì)胞 , NK-92細(xì)胞專用培養(yǎng)基
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Enhanced NK cell activation via eEF2K-mediated potentiation of the cGAS–STING pathway in hepatocellular carcinoma (2024-02-05)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.111628
影響因子 :5.6
引用產(chǎn)品: NK-92 細(xì)胞 , HCCLM3 細(xì)胞 , 人源NK細(xì)胞完全培養(yǎng)基
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Platelet-derived major histocompatibility complex class I coating on Treponema pallidum attenuates natural killer cell lethality (2024-05-14)
期刊:Virulence
DOI:10.1080/21505594.2024.2350892
影響因子 :5.2
引用產(chǎn)品: NK-92 細(xì)胞
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Co-Colorectal cancer stem cells employ the FADS1/DDA axis to evade NK cell-mediated immunosuppression after co-cultured with NK cells under hypoxia (2024-11-02)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.113535
影響因子 :4.8
引用產(chǎn)品: NK-92 細(xì)胞 , HT-29 細(xì)胞
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Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack (2024-12-30)
期刊:Journal of Zhejiang University-SCIENCE B
影響因子 :4.7
引用產(chǎn)品: NK-92 細(xì)胞 , NK-92細(xì)胞專用培養(yǎng)基
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Development of TSHR-CAR NK-92 Cells for Differentiated Thyroid Cancer (2024-04-24)
期刊:MOLECULAR AND CELLULAR ENDOCRINOLOGY
影響因子 :4.1
引用產(chǎn)品: NK-92 細(xì)胞
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A Pump-Free Microfluidic Co-Culture System for Investigating NK Cell-Tumor Spheroid Interactions in Flow Conditions (2024-11-15)
期刊:JOURNAL OF BIOTECHNOLOGY
DOI:10.1016/j.jbiotec.2024.11.008
影響因子 :4.1
引用產(chǎn)品: NK-92 細(xì)胞 , PNT1A 細(xì)胞 , NK-92細(xì)胞專用培養(yǎng)基 , DU 145 細(xì)胞
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The BCL2-associated athanogene-3–Interferon-induced transmembrane protein 2 axis enhances pancreatic ductal adenocarcinoma growth via the Mitogen-activated protein kinase signaling pathway (2024-08-30)
期刊:CARCINOGENESIS
影響因子 :3.3
引用產(chǎn)品: MIA PaCa-2 細(xì)胞 , PANC-1 細(xì)胞 , SU.86.86 細(xì)胞 , BxPC-3 細(xì)胞 , NK-92 細(xì)胞
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Regulation of the PD-1/PD-L1 Axis and NK Cell Dysfunction by Exosomal miR-552-5p in Gastric Cancer (2024-07-17)
期刊:DIGESTIVE DISEASES AND SCIENCES
DOI:10.1007/s10620-024-08536-0
影響因子 :2.5
引用產(chǎn)品: NK-92 細(xì)胞
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A biomimetic liver cancer on-a-chip reveals a critical role of LIPOCALIN-2 in promoting hepatocellular carcinoma progression (2023-05-04)
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2023.04.010
影響因子 :14.5
引用產(chǎn)品: LX-2 細(xì)胞 , 人臍靜脈內(nèi)皮細(xì)胞 , NK-92 細(xì)胞 , NK-92細(xì)胞專用培養(yǎng)基
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L-kynurenine induces NK cell loss in gastric cancer microenvironment via promoting ferroptosis (2023-03-01)
期刊:JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI:10.1186/s13046-023-02629-w
影響因子 :11.3
引用產(chǎn)品: NK-92 細(xì)胞
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Therapeutic effects against high-grade glioblastoma mediated by engineered induced neural stem cells combined with GD2-specific CAR-NK (2023-07-07)
期刊:CELLULAR ONCOLOGY
DOI:10.1007/s13402-023-00842-5
影響因子 :6.6
引用產(chǎn)品: U-87 MG 細(xì)胞 , NK-92 細(xì)胞 , MEM 培養(yǎng)基
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SHFM1 deficiency suppresses esophageal squamous cell carcinomas progression via modulating NF?κB signaling and enhancing nature killer cell?mediated tumor surveillance (2023-03-17)
期刊:Experimental and Therapeutic Medicine
影響因子 :2.7
引用產(chǎn)品: KYSE-410 細(xì)胞 , NK-92 細(xì)胞 , NK 92細(xì)胞專用培養(yǎng)基
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MMP-2 Inhibitor-Mediated Tumor Microenvironment Regulation Using a Sequentially Released Bio-Nanosystem for Enhanced Cancer Photo-Immunotherapy (2022-09-08)
期刊:ACS Applied Materials & Interfaces
影響因子 :10.4
引用產(chǎn)品: NK-92 細(xì)胞
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Natural killer cell-mimicking nanomaterial for overcoming the multidrug resistance of tumor via cascade catalysis (2022-10-14)
期刊:Science China-Materials
影響因子 :8.6
引用產(chǎn)品: NK-92 細(xì)胞 , MEMα (不含核苷) 培養(yǎng)基 , β-巰基乙醇 (55mM),1000× , 馬血清 , 特級(jí)胎牛血清 , 青霉素-鏈霉素溶液 (雙抗),100×
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Enhanced Proinflammatory Cytokine Production and Immunometabolic Impairment of NK Cells Exposed to Mycobacterium tuberculosis and Cigarette Smoke (2022-01-05)
期刊:Frontiers in Cellular and Infection Microbiology
影響因子 :5.5
引用產(chǎn)品: NK-92 細(xì)胞
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Presence of TSHR in NK Cells and Action of TSH on NK Cells (2022-01-25)
期刊:NEUROIMMUNOMODULATION
影響因子 :2.8
引用產(chǎn)品: NK-92 細(xì)胞
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PKCι regulates the expression of PDL1 through multiple pathways to modulate immune suppression of pancreatic cancer cells (2021-08-08)
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2021.110115
影響因子 :4.8
引用產(chǎn)品: NK-92 細(xì)胞
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Transcription factor Sp1 is upregulated by PKCι to drive the expression of YAP1 during pancreatic carcinogenesis (2021-04-17)
期刊:Carcinogenesis
影響因子 :4.7
引用產(chǎn)品: NK-92 細(xì)胞
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CD44 3-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression (2019-06-04)
期刊:International Journal of Biological Sciences
影響因子 :9.2
引用產(chǎn)品: NK-92 細(xì)胞
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