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產(chǎn)品概述
名稱 | KLE (人子宮內(nèi)膜癌細胞) (STR鑒定正確) |
別稱 | CVCL_1329 |
種屬 | 人 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 上皮細胞樣 |
凍存條件 | 凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:4 |
推薦換液頻率 | 2-3次/周 |
背景描述 | KLE細胞是源自一名64歲白人女性的子宮內(nèi)膜腫瘤組織,由G·R·Richardson于1984年建系。KLE細胞染色體為非整倍體,數(shù)目范圍在51-66之間。KLE細胞裸鼠植瘤后,其腫瘤標本在電鏡下顯示,細胞間見緊密相連,細胞表面具有微絨毛。KLE細胞形態(tài)特征與孕激素刺激后的子宮內(nèi)膜相似。 |
年齡(性別) | 女性;64歲 |
組織來源 | 子宮;子宮內(nèi)膜 |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 子宮內(nèi)膜腺癌細胞 |
生物安全等級 | BSL-1 |
倍增時間 | ~114 hours |
致瘤性 | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells. |
抗原表達情況 | Blood Type O; Rh+ |
保藏機構 | ATCC; CRL-1622 BCRJ; 0365 |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 13,14 D2S1338 18,19 D3S1358 17 D5S818 9,12 D7S820 11,12 D8S1179 8,14 D13S317 12 D16S539 11,12 D18S51 13,17 D19S433 15 D21S11 28,30 FGA 23,25 PentaD 13 PentaE 7 TH01 6,7 TPOX 8,11 vWA 16 D6S1043 13,14 D12S391 20,25 D2S441 14 -
STR鑒定圖
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參考文獻
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Multimodal near-infrared molecular imaging of ex vivo endometrial carcinoma via CD47-based targeted tracer (2025-02-04)
期刊:Bioengineering & Translational Medicine
影響因子 :6.1
引用產(chǎn)品: KLE 細胞 , HEC-1-A 細胞
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Sesamolin serves as an MYH14 inhibitor to sensitize endometrial cancer to chemotherapy and endocrine therapy via suppressing MYH9/GSK3β/β-catenin signaling (2024-05-02)
期刊:CELLULAR & MOLECULAR BIOLOGY LETTERS
DOI:10.1186/s11658-024-00583-9
影響因子 :9.2
引用產(chǎn)品: Ishikawa 細胞 , KLE 細胞
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TCR-mimicking STAR conveys superior sensitivity over CAR in targeting tumors with low-density neoantigens (2024-11-08)
期刊:Cell Reports
DOI:10.1016/j.celrep.2024.114949
影響因子 :7.5
引用產(chǎn)品: SK-BR-3 [SKBR3] 細胞 , KLE 細胞
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HMGB1-mediated transcriptional activation of circadian gene TIMELESS contributes to endometrial cancer progression through Wnt-β-catenin pathway (2024-01-10)
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2024.111045
影響因子 :4.8
引用產(chǎn)品: Ishikawa 細胞 , KLE 細胞 , Ishikawa細胞專用培養(yǎng)基 , KLE細胞專用培養(yǎng)基
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Long noncoding RNA VPS9D1-AS1 promotes the progression of endometrial cancer via regulation of the miR-187-3p/S100A4 axis (2024-07-02)
期刊:ENVIRONMENTAL TOXICOLOGY
影響因子 :4.4
引用產(chǎn)品: 人子宮內(nèi)膜上皮細胞 , HEC-1-A 細胞 , KLE 細胞 , Ishikawa 細胞 , RL95-2 細胞
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Lactamase β reprograms lipid metabolism to inhibit the progression of endometrial cancer through attenuating MDM2-mediated p53 ubiquitination and degradation (2024-12-31)
期刊:ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
影響因子 :3.8
引用產(chǎn)品: RL95-2 細胞 , HEC-1-A 細胞 , Ishikawa 細胞 , AN3 CA 細胞 , KLE 細胞
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Participation of Long Noncoding RNA FOXP4-AS1 in the Development and Progression of Endometrioid Carcinoma with Epigenetically Silencing DUSP5 (2024-03-20)
期刊:CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
影響因子 :2.4
引用產(chǎn)品: KLE 細胞 , RL95-2 細胞 , Ishikawa 細胞
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Activation of MAL2 by RAD21 inhibits the expression of MHC-I in immune evasion of endometrial cancer (2024-04-24)
期刊:CYTOTECHNOLOGY
DOI:10.1007/s10616-024-00629-y
影響因子 :2.2
引用產(chǎn)品: HEC-1-A 細胞 , KLE 細胞
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Asparanin A exerts cytotoxicity on human endometrial cancer Ishikawa cells via regulating miR-6236-p5_4 expression (2023-06-25)
期刊:FOOD AND CHEMICAL TOXICOLOGY
影響因子 :4.3
引用產(chǎn)品: KLE 細胞
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Novel insights into tumorigenesis and prognosis of endometrial cancer through systematic investigation and validation on mitophagy-related signature (2023-06-02)
期刊:Human Cell
DOI:10.1007/s13577-023-00920-8
影響因子 :4.3
引用產(chǎn)品: KLE 細胞
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Inhibition of ATM with KU-55933 Sensitizes Endometrial Cancer Cell Lines to Olaparib (2023-12-31)
期刊:OncoTargets and Therapy
影響因子 :4.0
引用產(chǎn)品: Ishikawa 細胞 , HEC-1-B 細胞 , AN3 CA 細胞 , KLE 細胞 , RL95-2 細胞
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The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma (2022-02-15)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2036912
影響因子 :4.9
引用產(chǎn)品: 人子宮內(nèi)膜上皮細胞 , KLE 細胞 , RL95-2 細胞 , Ishikawa 細胞
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Fatty Acid–Binding Protein 4 (FABP4) Suppresses Proliferation and Migration of Endometrial Cancer Cells via PI3K/Akt Pathway (2021-06-29)
期刊:OncoTargets and Therapy
影響因子 :4.4
引用產(chǎn)品: KLE 細胞 , McCoy's 5A 培養(yǎng)基
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OSU-03012 Disrupts Akt Signaling and Prevents Endometrial Carcinoma Progression in vitro and in vivo (2021-04-30)
期刊:Drug Design Development and Therapy
影響因子 :4.3
引用產(chǎn)品: KLE 細胞 , McCoy's 5A 培養(yǎng)基 , McCoy's 5A 培養(yǎng)基
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hsa_circ_0001610 knockdown modulates miR-646-STAT3 axis to suppress endometrial carcinoma progression (2021-04-20)
期刊:Journal Of Gene Medicine
DOI:10.1002/jgm.3337
影響因子 :3.5
引用產(chǎn)品: HEC-1-B 細胞 , KLE 細胞 , Ishikawa 細胞
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Exosomal-lncRNA DLEU1 Accelerates the Proliferation, Migration, and Invasion of Endometrial Carcinoma Cells by Regulating microRNA-E2F3 (2020-08-25)
期刊:OncoTargets and Therapy
影響因子 :4.0
引用產(chǎn)品: KLE 細胞 , Ishikawa 細胞 , HEC-1-A 細胞 , RL95-2 細胞
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Silencing of Long Non-Coding RNA LINC01106 Suppresses the Proliferation, Migration and Invasion of Endometrial Cancer Cells Through Regulating the miR-449a/MET Axis (2020-09-29)
期刊:OncoTargets and Therapy
影響因子 :4.0
引用產(chǎn)品: KLE 細胞 , Ishikawa 細胞 , HEC-1-A 細胞
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Knockdown of Trop2 inhibits proliferation and migration and induces apoptosis of endometrial cancer cells via AKT/β‐catenin pathway (2020-01-22)
期刊:Cell Biochemistry and Function
DOI:10.1002/cbf.3450
影響因子 :3.6
引用產(chǎn)品: Ishikawa 細胞 , KLE 細胞 , 人子宮內(nèi)膜上皮細胞
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