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0.25%胰蛋白酶溶液(含EDTA,溶于D-Hank's)
貨號:PB180227
規(guī)格:100mL
價格: ¥68
產(chǎn)品概述
胰蛋白酶(Trypsin)是一種絲氨酸水解酶,它能把多肽鏈中賴氨酸和精氨酸殘基中的羧基側(cè)切段,水解細胞間的蛋白質(zhì),破壞細胞間的連接,從而使組織或貼壁細胞離散成單個細胞。胰酶分散細胞的活性與組織或細胞的特性、胰酶濃度、溫度和作用時間有關(guān),在pH 8.0和37℃時,胰酶的作用能力最強,因此使用胰酶時,應(yīng)把握好濃度、溫度和時間,以免消化過度造成細胞損傷。
一般常用胰酶的工作濃度為0.25%,而半貼壁細胞或?qū)σ让该舾械募毎2捎玫蜐舛龋?/span>0.05%)的胰酶進行細胞消化。由于EDTA能夠螯合Ca2+和Mg2+,從而破壞細胞連接促進細胞的解離,因此在胰酶溶液中常常會加入一定量的EDTA混合使用,以增強解離效果。
產(chǎn)品信息
形態(tài) | 液體 |
規(guī)格 | 100mL |
pH | 7.2-7.4 |
溶劑 | D-Hank's |
胰酶蛋白酶濃度 | 2.5 g/L |
EDTA?2Na | 0.2 g/L |
酚紅指示劑 | 無 |
儲存條件 | -5~-20℃ |
運輸條件 | 低溫 |
有效期 | 12個月 |
注意事項
1、由于不同的組織或者細胞對胰酶的作用反應(yīng)不一樣,因此操作人員應(yīng)根據(jù)實際情況,確定最佳消化時間;消化細胞時間不宜過長,否則會影響細胞貼壁和生長狀況;
2、使用本產(chǎn)品時應(yīng)注意無菌操作,避免污染;
3、不宜長時間放置于室溫環(huán)境或2-8℃長期保存;
4、2-8℃中解凍,搖勻后使用,切忌反復(fù)凍融,用量較少時建議分裝凍存。
5、由于D-Hank's平衡鹽溶液NaHCO3含量較低(0.35g/L),因此該型胰蛋白酶溶液不能用于5% CO2的環(huán)境,若放入CO2培養(yǎng)箱,溶液將迅速變酸,使用時應(yīng)注意;
6、本產(chǎn)品僅用于科研或進一步研究使用,不用于診斷和治療。
參考文獻
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引用產(chǎn)品: MC3T3-E1 Subclone 24 細胞 , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS)
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Network pharmacology and experimental validation of Maxing Shigan decoction in the treatment of influenza virus-induced ferroptosis (2023-10-23)
期刊:Chinese Journal of Natural Medicines
DOI:10.1016/S1875-5364(23)60457-1
影響因子 :4.6
引用產(chǎn)品: RPMI-1640 培養(yǎng)基 , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS)
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Fractionation of Simulated Digestive Products of Bird’s Nest In Vitro: Skin Whitening Activity of Fractions (2023-08-25)
期刊:JOURNAL OF FOOD BIOCHEMISTRY
影響因子 :4.0
引用產(chǎn)品: RPMI-1640 培養(yǎng)基 , 特級胎牛血清 , Dulbecco's磷酸鹽緩沖液 (DPBS),不含鈣、鎂、酚紅 , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS) , B16-F10 細胞
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Mechanisms of Cd-Induced Cytotoxicity in Normal Human Skin Keratinocytes: Implication for Human Health (2022-10-04)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影響因子 :6.2
引用產(chǎn)品: MEM 培養(yǎng)基 , 特級胎牛血清 , 青霉素-鏈霉素溶液(雙抗),100× , 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
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Superwettable and injectable GelMA-MSC microspheres promote cartilage repair in temporomandibular joints (2022-09-20)
期刊:Frontiers in Bioengineering and Biotechnology
DOI:10.3389/fbioe.2022.1026911
影響因子 :6.1
引用產(chǎn)品: 特級胎牛血清 , 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
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A pH-responsive chiral mesoporous silica nanoparticles for delivery of doxorubicin in tumor-targeted therapy (2022-11-17)
期刊:COLLOIDS AND SURFACES B-BIOINTERFACES
DOI:10.1016/j.colsurfb.2022.113027
影響因子 :6.0
引用產(chǎn)品: 0.25%胰蛋白酶溶液(含EDTA,溶于PBS) , DAPI 染色液(25 μg/mL) , RPMI-1640 培養(yǎng)基 , 特級胎牛血清 , 青霉素-鏈霉素溶液(雙抗),100×
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