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產(chǎn)品概述
HEPES全稱為N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid，中文名稱為4-羥乙基哌嗪乙磺酸，是一種常用的生物緩沖劑，pKa (25℃)=7.5，有效緩沖范圍為pH 6.8-8.2。HEPES最大的優(yōu)點(diǎn)是，能夠在開放環(huán)境中較長時(shí)間恒定溶液的PH范圍。HEPES不能通過生物膜，對生化反應(yīng)影響有限，是一種化學(xué)和酶穩(wěn)定的物質(zhì)，而且HEPES對可見光和紫外光的吸收度極低。因此一般認(rèn)為，HEPES是一種優(yōu)良的生物緩沖劑，對細(xì)胞無毒性作用，使用終濃度為10-50 mM，細(xì)胞培養(yǎng)常用10 mM。
產(chǎn)品信息
形態(tài) | 液體 |
濃度 | 1 M |
規(guī)格 | 100mL |
PH | 7.2-7.4 |
溶劑 | 純化水 |
HEPES | 238.3 g/L |
儲存條件 | 2-8℃或-5~-20℃，避光。 |
運(yùn)輸條件 | 常溫 |
有效期 | 2-8℃，24個(gè)月；-5~-20℃，長期。 |
注意事項(xiàng)
1、本產(chǎn)品經(jīng)0.1 μm過濾除菌；
2、使用本產(chǎn)品時(shí)應(yīng)注意無菌操作，避免污染；
3、使用后請及時(shí)避光保存，有研究報(bào)道培養(yǎng)液中的HEPES在可見光下暴露3h以上將產(chǎn)生對細(xì)胞有毒性的過氧化氫；
4、本產(chǎn)品僅用于科研或進(jìn)一步研究使用，不用于診斷和治療。
參考文獻(xiàn)
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影響因子 ：7.0
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期刊：Molecular Oncology
影響因子 ：5.0
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FOXM1 Participates in Scleral Remodeling in Myopia by Upregulating APOA1 Expression Through METTL3/YTHDF2 (2024-01-02)
期刊：INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
影響因子 ：4.4
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LncRNA HOTTIP regulates TLR4 promoter methylation by recruiting H3K4 methyltransferase MLL1 to affect apoptosis and inflammatory response of fibroblast-like synoviocyte in rheumatoid arthritis (2024-02-16)
期刊：KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
影響因子 ：3.3
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Evaluating the Diagnostic Potential of SOCS3 in Copper Metabolism for Acute Myocardial Infarction (2024-10-28)
期刊：PATHOLOGY RESEARCH AND PRACTICE
影響因子 ：2.9
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TREK-1 channel as a therapeutic target for dexmedetomidine-mediated neuroprotection in cerebral ischemia (2024-07-08)
期刊：NEUROGENETICS
DOI：10.1007/s10048-024-00772-w
影響因子 ：1.6
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Remodeling Collagen Microenvironment in Liver Using a Biomimetic Nano-Regulator for Reversal of Liver Fibrosis (2023-04-23)
期刊：Advanced Science
影響因子 ：15.1
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Role of macrophage AHR/TLR4/STAT3 signaling axis in the colitis induced by non-canonical AHR ligand aflatoxin B1 (2023-03-23)
期刊：JOURNAL OF HAZARDOUS MATERIALS
DOI：10.1016/j.jhazmat.2023.131262
影響因子 ：13.6
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期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.110137
影響因子 ：5.6
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影響因子 ：5.5
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期刊：COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
DOI：10.1016/j.colsurfa.2023.131904
影響因子 ：5.2
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期刊：CELLULAR SIGNALLING
DOI：10.1016/j.cellsig.2023.110840
影響因子 ：4.8
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期刊：ENVIRONMENTAL TOXICOLOGY
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期刊：Brazilian Journal of Otorhinolaryngology
DOI：10.1016/j.bjorl.2022.06.005
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DOI：10.1158/1541-7786.MCR-22-0231
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DOI：10.1007/s11064-021-03508-6
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DOI：10.1007/s10147-022-02231-8
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DOI：10.1016/j.imbio.2022.152179
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影響因子 ：8.8
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影響因子 ：6.8
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DOI：10.1080/15384101.2020.1823730
影響因子 ：4.3
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期刊：Digestive Diseases and Sciences
DOI：10.1007/s10620-020-06435-8
影響因子 ：3.1
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期刊：Journal Of Cellular Physiology
影響因子 ：5.6
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期刊：JOURNAL OF CELLULAR PHYSIOLOGY
影響因子 ：4.5
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期刊：Cell Cycle
DOI：10.1080/15384101.2019.1578144
影響因子 ：4.3
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期刊：Cell Cycle
DOI：10.1080/15384101.2019.1629774
影響因子 ：4.3
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Overexpression of miR-150 Inhibits the NF-κB Signal Pathway in Intervertebral Disc Degeneration through Targeting P2X7 (2019-11-14)
期刊：Cells Tissues Organs
影響因子 ：2.7
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期刊：Journal Of Cellular Physiology
影響因子 ：5.6
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Inhibition of microRNA‐129‐5p expression ameliorates ultraviolet ray‐induced corneal epithelial cell injury via upregulation of EGFR (2018-12-04)
期刊：Journal Of Cellular Physiology
影響因子 ：5.6
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MicroRNA-488 inhibits progression of colorectal cancer via inhibition of the mitogen-activated protein kinase pathway by targeting claudin-2 (2018-09-12)
期刊：American Journal Of Physiology-Cell Physiology
DOI：10.1152/ajpcell.00047.2018
影響因子 ：5.5
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Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via the Wnt/β-catenin signaling pathway (2018-11-01)
期刊：American Journal of Physiology-Cell Physiology
DOI：10.1152/ajpcell.00291.2017
影響因子 ：5.5
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Retracted: S100A9 gene silencing inhibits the release of pro‐inflammatory cytokines by blocking the IL‐17 signalling pathway in mice with acute pancreatitis (2018-02-14)
期刊：Journal Of Cellular And Molecular Medicine
影響因子 ：5.3
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MicroRNA-23a inhibits melanoma cell proliferation, migration, and invasion in mice through a negative feedback regulation of sdcbp and the MAPK/ERK signaling pathway (2018-12-27)
期刊：Iubmb Life
DOI：10.1002/iub.1979
影響因子 ：4.6
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Retracted: MicroRNA-381-induced down-regulation of CXCR4 promotes the proliferation of renal tubular epithelial cells in rat models of renal ischemia reperfusion injury (2018-01-02)
期刊：Journal Of Cellular Biochemistry
影響因子 ：4.0
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期刊：Bioscience Reports
影響因子 ：4.0
引用產(chǎn)品： HEPES液 (1M)
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MicroRNA-182 downregulates Wnt/β-catenin signaling, inhibits proliferation, and promotes apoptosis in human osteosarcoma cells by targeting HOXA9 (2017-09-22)
期刊：Oncotarget
影響因子 ：0.0
引用產(chǎn)品： HEPES液 (1M)
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