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細胞培養(yǎng)最優(yōu)解!
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產品概述
名稱 | TM3 (小鼠睪丸間質細胞) (STR鑒定正確) |
別稱 | TM-3 |
種屬 | 小鼠 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 上皮細胞樣 |
凍存條件 | 凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:8 |
推薦換液頻率 | 2-3次/周 |
注意事項 | 該細胞貼壁松散,操作時請盡量輕柔;換液時需預熱培養(yǎng)基;收貨如有大塊脫落的細胞團,為正?,F(xiàn)象,請按照收貨注意事項處理。 |
背景描述 | TM3細胞在LH作用下cAMP產量升高,但對促卵泡激素沒有響應。TM3細胞對LH的響應持續(xù)時間與血清批次有關;在LH存在下,TM3細胞可以代謝膽固醇。 |
年齡(性別) | 雄性;11-13天 |
組織來源 | 睪丸間質 |
細胞類型 | 自發(fā)永生化細胞 |
生物安全等級 | BSL-1 |
致瘤性 | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
受體表達情況 | Luteinizing hormone (LH); epidermal growth factor (EGF); androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive |
基因表達情況 | prostaglandin F2a |
保藏機構 | ATCC; CRL-1714 ECACC; 91060526 |
STR鑒定
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STR位點信息
18-3 18 6-7 15 5-5 11,12 X-1 24 1-2 15,17 7-1 25.2,26.2 8-1 13,16,17 1-1 16 19-2 9 15-3 20.3,22.3 12-1 16 6-4 16.3,18 4-2 19.3 3-2 13,14 2-1 9,16 13-1 16.2
11-2 15,18 17-2 15,16 -
STR鑒定圖
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參考文獻
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Perfluorooctanoic acid induces Leydig cell injury via inhibition of autophagosomes formation and activation of endoplasmic reticulum stress (2024-01-06)
期刊:SCIENCE OF THE TOTAL ENVIRONMENT
DOI:10.1016/j.scitotenv.2023.169861
影響因子 :8.2
引用產品: TM3 細胞
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Anti-Cancer Potency of Copper-Doped Carbon Quantum Dots Against Breast Cancer Progression (2024-02-26)
期刊:International Journal of Nanomedicine
影響因子 :8.0
引用產品: NRK-52E 細胞 , TM3 細胞 , MA-782細胞專用培養(yǎng)基 , TM3細胞專用培養(yǎng)基 , DMEM高糖完全培養(yǎng)基 (含10%FBS)
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DEHP-mediated oxidative stress leads to impaired testosterone synthesis in Leydig cells through the cAMP/PKA/SF-1/StAR pathway (2024-12-08)
期刊:ENVIRONMENTAL POLLUTION
DOI:10.1016/j.envpol.2024.125503
影響因子 :7.6
引用產品: TM3 細胞 , TM3細胞專用培養(yǎng)基
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Tris(2-ethylhexyl) phosphate induces cytotoxicity in TM3 Leydig cells by modulating autophagy and endoplasmic reticulum stress (2024-05-21)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2024.116462
影響因子 :6.8
引用產品: TM3 細胞
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Effect of Stem Extract of Schisandra Chinensis on Improving Spermatogenesis Disorder Induced by Cisplatin in Vivo and in Vitro (2024-11-22)
期刊:PHYTOMEDICINE
DOI:10.1016/j.phymed.2024.156274
影響因子 :6.7
引用產品: TM3 細胞
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Rotenone-induced cell apoptosis via endoplasmic reticulum stress and PERK-eIF2α-CHOP signalling pathways in TM3 cells (2024-09-03)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2024.116972
影響因子 :6.2
引用產品: TM3 細胞
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Melatonin alleviates oxidative stress damage in mouse testes induced by bisphenol A (2024-02-19)
期刊:Frontiers in Cell and Developmental Biology
DOI:10.3389/fcell.2024.1338828
影響因子 :5.5
引用產品: TM3 細胞
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Hexafluoropropylene oxide trimer acid (HFPO-TA) exerts cytotoxic effects on leydig cells via the ER stress/JNK/β-trcp/mcl-1 axis (2024-04-20)
期刊:FOOD AND CHEMICAL TOXICOLOGY
影響因子 :3.9
引用產品: TM3 細胞
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Chronic Polystyrene Microplastic Exposure Reduces Testosterone Levels in Mice through Mitochondrial Oxidative Stress and BAX/BCL2-Mediated Apoptosis (2024-08-01)
期刊:Toxics
影響因子 :3.9
引用產品: TM3 細胞
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Oligoasthenozoospermia is alleviated in a mouse model by [Gly14]-humanin-mediated attenuation of oxidative stress and ferroptosis (2024-10-22)
期刊:Andrology
影響因子 :3.2
引用產品: 特級胎牛血清 , GC-1 spg 細胞 , TM3 細胞 , TM4 細胞 , GC-2spd(ts) 細胞
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MicroRNA-361-5p Alleviates Leydig Cell Apoptosis and Promotes Cell Growth by Targeting PIAS1 in Late-Onset Hypogonadism (2024-06-11)
期刊:MOLECULAR BIOTECHNOLOGY
DOI:10.1007/s12033-024-01174-x
影響因子 :2.4
引用產品: TM3 細胞 , DMEM/F12 培養(yǎng)基
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Toxic effects of Tripterygium glycoside tablets on the reproductive system of male rats by metabolomics, cytotoxicity, and molecular docking (2023-04-10)
期刊:PHYTOMEDICINE
DOI:10.1016/j.phymed.2023.154813
影響因子 :7.9
引用產品: TM3細胞專用培養(yǎng)基 , TM4細胞專用培養(yǎng)基 , TM3 細胞 , TM4 細胞
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1-Nitropyrene disrupts testicular steroidogenesis via oxidative stress-evoked PERK-eIF2α pathway (2023-05-17)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2023.115027
影響因子 :6.8
引用產品: TM3 細胞 , TM3細胞專用培養(yǎng)基
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The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis (2023-07-20)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2023.115262
影響因子 :6.8
引用產品: TM4 細胞 , TM3 細胞 , GC-1 spg 細胞 , GC-2spd (ts) 細胞 , DMEM/F12 培養(yǎng)基 , DMEM高糖 培養(yǎng)基
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Triphenyl phosphate induced apoptosis of mice testicular Leydig cells and TM3 cells through ROS-mediated mitochondrial fusion inhibition (2023-04-05)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2023.114876
影響因子 :6.8
引用產品: TM3 細胞
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Phytochemical analysis and in vitro and in vivo antioxidant properties of Plagiorhegma dubia Maxim as a medicinal crop for diabetes treatment (2023-03-09)
期刊:Arabian Journal of Chemistry
DOI:10.1016/j.arabjc.2023.104788
影響因子 :6.0
引用產品: TM3 細胞
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Prenatal DEHP Exposure Induces Premature Testicular Aging by Promoting Leydig Cell Senescence through the MAPK Signaling Pathways (2023-05-28)
期刊:Advanced Biology
影響因子 :3.7
引用產品: TM3 細胞
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Rosmarinic acid ameliorates autoimmune responses through suppression of intracellular nucleic acid-mediated type I interferon expression (2023-05-26)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2023.05.113
影響因子 :3.1
引用產品: TM3 細胞
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Knockdown of ZnT4 Induced Apoptosis, Inhibited Proliferation and testosterone synthesis of TM3 cells (2023-09-21)
期刊:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI:10.1007/s11626-023-00804-z
影響因子 :2.1
引用產品: TM3 細胞 , TM3細胞專用培養(yǎng)基
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MicroRNA profiling reveals the role of miR-133b-3p in promoting apoptosis and inhibiting cell proliferation and testosterone synthesis in mouse TM3 cells (2023-01-30)
期刊:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI:10.1007/s11626-022-00745-z
影響因子 :2.1
引用產品: TM3 細胞
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Glyphosate-induced mitochondrial reactive oxygen species overproduction activates parkin-dependent mitophagy to inhibit testosterone synthesis in mouse leydig cells (2022-09-29)
期刊:ENVIRONMENTAL POLLUTION
DOI:10.1016/j.envpol.2022.120314
影響因子 :10.0
引用產品: TM3 細胞
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Melatonin inhibits autophagy in TM3 cells via AKT/FOXO1 pathway (2022-01-08)
期刊:Molecular Biology Reports
DOI:10.1007/s11033-021-07107-0
影響因子 :2.8
引用產品: TM3 細胞
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Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders (2021-10-20)
期刊:Journal of Hazardous Materials
DOI:10.1016/j.jhazmat.2021.127547
影響因子 :14.2
引用產品: TM3 細胞 , TM3細胞專用培養(yǎng)基
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1,25-Dihydroxyvitamin D deficiency accelerates male reproductive senescence in aging mice and 1,25(OH)2D3 alleviates oxidative stress via NF-κB/SOD (2021-04-07)
期刊:American Journal Of Physiology-Endocrinology And Metabolism
DOI:10.1152/ajpendo.00531.2020
影響因子 :5.1
引用產品: TM3 細胞
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A Protein from Dioscorea polystachya (Chinese Yam) Improves Hydrocortisone-Induced Testicular Dysfunction by Alleviating Leydig Cell Injury via Upregulation of the Nrf2 Pathway (2021-11-30)
期刊:Oxidative Medicine and Cellular Longevity
影響因子 :0.0
引用產品: TM3 細胞 , TM3細胞專用培養(yǎng)基
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Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells (2020-08-14)
期刊:Cell Death & Disease
DOI:10.1038/s41419-020-02751-z
影響因子 :9.0
引用產品: TM3 細胞
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Melatonin inhibits apoptosis in mouse Leydig cells via the retinoic acid-related orphan nuclear receptor α/p53 pathway (2020-02-13)
期刊:Life Sciences
影響因子 :6.1
引用產品: TM3 細胞
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Antioxidant and enzyme-inhibitory activity of extracts from Erigeron annuus flower (2020-03-03)
期刊:INDUSTRIAL CROPS AND PRODUCTS
DOI:10.1016/j.indcrop.2020.112283
影響因子 :4.2
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BMAL1 knockdown promoted apoptosis and reduced testosterone secretion in TM3 Leydig cell line (2020-04-16)
期刊:Gene
DOI:10.1016/j.gene.2020.144672
影響因子 :3.5
引用產品: TM3 細胞
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Melatonin Inhibits Apoptosis and Oxidative Stress of Mouse Leydig Cells via a SIRT1-Dependent Mechanism (2019-08-25)
期刊:Molecules.
影響因子 :4.6
引用產品: TM3 細胞
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