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產(chǎn)品概述
| 名稱 | GC-1 spg (小鼠精原細(xì)胞) (種屬鑒定正確) |
| 別稱 | GC-1spg; GC-1; GC1-SPG |
| 種屬 | 小鼠 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
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| 推薦傳代比例 | 1:4-1:6 |
| 推薦換液頻率 | 2-3次/周 |
| 背景描述 | Type B spermatogonia were immortalized by transfection with pSV3-neo (a plasmid containing coding sequences for the SV40 large T antigen and neomycin resistance) |
| 年齡（性別） | 雄性；10日齡 |
| 組織來源 | 睪丸 |
| 細(xì)胞類型 | 轉(zhuǎn)化細(xì)胞系 |
| 生物安全等級 | BSL-2 |
| 保藏機(jī)構(gòu) | ATCC; CRL-2053 |
STR鑒定
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STR位點(diǎn)信息
18-3 17,19 6-7 12,13 5-5 14 X-1 25 1-2 13 7-1 29 8-1 15 1-1 10 19-2 11,12 15-3 20.3 12-1 20 6-4 16 4-2 19.3,20.3 3-2 14 2-1 9 13-1 16.2,17.2
11-2 15,17 17-2 13,14 -
STR鑒定圖
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參考文獻(xiàn)
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Gut-Derived Exosomes Mediate the Microbiota Dysbiosis-Induced Spermatogenesis Impairment by Targeting Meioc in Mice (2024-03-25)
期刊：Advanced Science
影響因子 ：15.1
引用產(chǎn)品： GC-1 spg 細(xì)胞 , GC-2spd (ts) 細(xì)胞
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Sperm miR-142-3p Reprogramming Mediates Paternal Pre-Pregnancy Caffeine Exposure-Induced Non-Alcoholic Steatohepatitis in Male Offspring Rats (2024-09-18)
期刊：Advanced Science
影響因子 ：14.3
引用產(chǎn)品： GC-1 spg 細(xì)胞
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Chronic exposure to liquid crystal monomer EBCN at environmentally relevant concentrations induces testicular dysfunction via the gut-testis axis (2024-12-28)
期刊：JOURNAL OF HAZARDOUS MATERIALS
DOI：10.1016/j.jhazmat.2024.137033
影響因子 ：12.2
引用產(chǎn)品： GC-1 spg 細(xì)胞
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Short Chain Chlorinated Paraffins Impaired Spermatogenesis Process in Mice via Inhibiting α-KG/TET Enzyme Activity (2024-09-19)
期刊：ENVIRONMENTAL SCIENCE & TECHNOLOGY
影響因子 ：10.8
引用產(chǎn)品： GC-1 spg 細(xì)胞
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The necroptosis-mediated imbalance of mitochondrial dynamics is involved in DEHP-induced toxicity to immature testes via the PGAM5–DRP1 interaction (2024-02-15)
期刊：ENVIRONMENTAL POLLUTION
DOI：10.1016/j.envpol.2024.123585
影響因子 ：8.9
引用產(chǎn)品： GC-1 spg 細(xì)胞 , GC-2spd (ts) 細(xì)胞
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Diquat causes mouse testis injury through inducing heme oxygenase-1-mediated ferroptosis in spermatogonia (2024-06-08)
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2024.116562
影響因子 ：6.2
引用產(chǎn)品： GC-1 spg 細(xì)胞
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DCAF2 regulates the proliferation and differentiation of mouse progenitor spermatogonia by targeting p21 and thymine DNA glycosylase (2024-06-04)
期刊：CELL PROLIFERATION
影響因子 ：5.9
引用產(chǎn)品： GC-1 spg 細(xì)胞
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Ferritinophagy-mediated ferroptosis of spermatogonia is involved in busulfan-induced oligospermia in the mice (2024-01-12)
期刊：CHEMICO-BIOLOGICAL INTERACTIONS
影響因子 ：5.1
引用產(chǎn)品： GC-1 spg 細(xì)胞
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Polystyrene microplastics induce male reproductive toxicity in mice by activating spermatogonium mitochondrial oxidative stress and apoptosis (2024-05-10)
期刊：CHEMICO-BIOLOGICAL INTERACTIONS
影響因子 ：4.7
引用產(chǎn)品： GC-1 spg 細(xì)胞
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Testicular dysfunction and “its recovery effect” after cadmium exposure (2024-04-12)
期刊：FOOD AND CHEMICAL TOXICOLOGY
影響因子 ：4.3
引用產(chǎn)品： GC-1 spg 細(xì)胞
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Oligoasthenozoospermia is alleviated in a mouse model by [Gly14]-humanin-mediated attenuation of oxidative stress and ferroptosis (2024-10-22)
期刊：Andrology
影響因子 ：3.2
引用產(chǎn)品： 特級胎牛血清 , GC-1 spg 細(xì)胞 , TM3 細(xì)胞 , TM4 細(xì)胞 , GC-2spd(ts) 細(xì)胞
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Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy (2023-03-20)
期刊：JOURNAL OF HAZARDOUS MATERIALS
DOI：10.1016/j.jhazmat.2023.131234
影響因子 ：13.6
引用產(chǎn)品： GC-1 spg 細(xì)胞
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Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1?cells: Insights from multiple transcriptomic profiling (2023-06-24)
期刊：ENVIRONMENTAL POLLUTION
DOI：10.1016/j.envpol.2023.122091
影響因子 ：8.9
引用產(chǎn)品： GC-1 spg 細(xì)胞
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Puerarin improves busulfan-induced disruption of spermatogenesis by inhibiting MAPK pathways (2023-07-27)
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.115231
影響因子 ：7.5
引用產(chǎn)品： GC-1 spg 細(xì)胞 , TM4 細(xì)胞 , DMEM高糖 培養(yǎng)基 , 青霉素-鏈霉素溶液 (雙抗)，100× , 0.25%胰蛋白酶溶液 (含酚紅，溶于D-Hank's)
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The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis (2023-07-20)
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2023.115262
影響因子 ：6.8
引用產(chǎn)品： TM4 細(xì)胞 , TM3 細(xì)胞 , GC-1 spg 細(xì)胞 , GC-2spd (ts) 細(xì)胞 , DMEM/F12 培養(yǎng)基 , DMEM高糖 培養(yǎng)基
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X-box binding protein 1 caused an imbalance in pyroptosis and mitophagy in immature rats with di-(2-ethylhexyl) phthalate-induced testis toxicity (2023-03-27)
期刊：Genes & Diseases
DOI：10.1016/j.gendis.2023.02.030
影響因子 ：6.8
引用產(chǎn)品： GC-1 spg 細(xì)胞 , GC-2spd (ts) 細(xì)胞
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Loss of histone reader Phf7 leads to immune pathways activation via endogenous retroviruses during spermiogenesis (2023-09-25)
期刊：iScience
DOI：10.1016/j.isci.2023.108030
影響因子 ：5.8
引用產(chǎn)品： GC-1 spg 細(xì)胞 , GC-2spd(ts) 細(xì)胞
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Melatonin Ameliorates Diquat-Induced Testicular Toxicity via Reducing Oxidative Stress, Inhibiting Apoptosis, and Maintaining the Integrity of Blood-Testis Barrier in Mice (2023-02-08)
期刊：Toxics
影響因子 ：4.6
引用產(chǎn)品： GC-1 spg 細(xì)胞
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BNC1 deficiency induces mitochondrial dysfunction-triggered spermatogonia apoptosis through the CREB/SIRT1/FOXO3 pathway: the therapeutic potential of nicotinamide riboside and metformin (2023-12-11)
期刊：BIOLOGY OF REPRODUCTION
影響因子 ：3.6
引用產(chǎn)品： GC-1 spg 細(xì)胞
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miR-124-3p regulates the involvement of Ptpn1 in testicular development and spermatogenesis in mouse (2023-11-04)
期刊：GENE
DOI：10.1016/j.gene.2023.147967
影響因子 ：3.5
引用產(chǎn)品： GC-1 spg 細(xì)胞
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Epigallocatechin gallate alleviates mono-2-ethylhexyl phthalate-induced male germ cell pyroptosis by inhibiting the ROS/mTOR/NLRP3 pathway (2023-06-05)
期刊：TOXICOLOGY IN VITRO
影響因子 ：3.2
引用產(chǎn)品： GC-1 spg 細(xì)胞 , GC-2spd (ts) 細(xì)胞
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Effect of Low Dietary Folate on Mouse Spermatogenesis and Spindle Assembly Checkpoint Dysfunction May Contribute to Folate Deficiency-Induced Chromosomal Instability in Cultured Mouse Spermatogonia (2023-06-08)
期刊：DNA AND CELL BIOLOGY
影響因子 ：3.1
引用產(chǎn)品： GC-1 spg 細(xì)胞
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Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation (2022-09-16)
期刊：CELL DEATH AND DIFFERENTIATION
DOI：10.1038/s41418-022-01057-1
影響因子 ：12.1
引用產(chǎn)品： GC-1 spg 細(xì)胞
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The protective effect of C-phycocyanin in male mice reproductive system (2022-01-28)
期刊：Food & Function
影響因子 ：6.1
引用產(chǎn)品： GC-1 spg 細(xì)胞
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The mechanism analysis using PI3K/AKT pathway for the effects of levocarnitine in the treatment of spermatogenic dysfunction (2021-10-28)
期刊：Andrologia
影響因子 ：2.5
引用產(chǎn)品： GC-1 spg 細(xì)胞 , 青霉素-鏈霉素溶液 (雙抗)，100×
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