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產(chǎn)品概述
名稱 | TM4 (正常小鼠睪丸Sertoli細(xì)胞) (STR鑒定正確) |
別稱 | TM-4 |
種屬 | 小鼠 |
生長特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:8 |
推薦換液頻率 | 2-3次/周 |
注意事項(xiàng) | 該細(xì)胞貼壁松散,操作時(shí)請盡量輕柔;換液時(shí)需預(yù)熱培養(yǎng)基;收貨如有大塊脫落的細(xì)胞團(tuán),為正常現(xiàn)象,請按照收貨注意事項(xiàng)處理。 |
背景描述 | TM4細(xì)胞在FSH的作用下cAMP產(chǎn)量增加,但對LH無反應(yīng);TM4細(xì)胞與原代Sertoli細(xì)胞相比,對FSH的反應(yīng)性降低。據(jù)報(bào)道,TM4細(xì)胞組成性纖溶酶原激活物的產(chǎn)量很低,但可被FSH刺激產(chǎn)生,受維甲酸刺激可有大幅增高。TM4細(xì)胞可產(chǎn)生視黃醇結(jié)合蛋白、組織纖溶酶原激活物和轉(zhuǎn)鐵蛋白;TM4細(xì)胞表達(dá)FSH受體、雄激素受體和孕激素受體;鼠痘病毒陰性。 |
年齡(性別) | 雄性;11-13日齡 |
組織來源 | 睪丸 |
細(xì)胞類型 | 自發(fā)永生化細(xì)胞 |
生物安全等級 | BSL-1 |
致瘤性 | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
基因表達(dá)情況 | retinol binding protein, tissue plasminogen activator, transferrin |
保藏機(jī)構(gòu) | ATCC; CRL-1715 BCRC; 60254 ECACC; 88111401 |
STR鑒定
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STR位點(diǎn)信息
18-3 18 6-7 12,15 5-5 15 X-1 24,25 1-2 15,17 7-1 24.2,26.2 8-1 16 1-1 15,16 19-2 9 15-3 20.3,21.3,22.3 12-1 20 6-4 18,19 4-2 19.3 3-2 11,14 2-1 9,16 13-1 15.1
11-2 15 17-2 15 -
STR鑒定圖
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參考文獻(xiàn)
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Polylactic Acid Micro/Nanoplastic Exposure Induces Male Reproductive Toxicity by Disrupting Spermatogenesis and Mitochondrial Dysfunction in Mice (2025-01-27)
期刊:ACS Nano
影響因子 :15.8
引用產(chǎn)品: TM4 細(xì)胞 , GC-2spd(ts) 細(xì)胞
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Mitochondrial ROS-associated integrated stress response is involved in arsenic-induced blood-testis barrier disruption and protective effect of melatonin (2025-02-23)
期刊:ENVIRONMENT INTERNATIONAL
DOI:10.1016/j.envint.2025.109346
影響因子 :10.3
引用產(chǎn)品: TM4 細(xì)胞
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Lead Causes Lipid Droplet Accumulation by Impairing Lysosomal Function and Autophagic Flux in Testicular Sertoli Cells (2025-02-28)
期刊:Toxics
影響因子 :3.9
引用產(chǎn)品: TM4 細(xì)胞
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Prenatal dexamethasone exposure impairs rat blood-testis barrier function and sperm quality in adult offspring via GR/KDM1B/FSTL3/TGFβ signaling (2024-03-12)
期刊:ACTA PHARMACOLOGICA SINICA
DOI:10.1038/s41401-024-01244-5
影響因子 :8.2
引用產(chǎn)品: TM4 細(xì)胞
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Lycium barbarum polysaccharide alleviates ferroptosis in Sertoli cells through NRF2/SLC7A11/GPX4 pathway and ameliorates DEHP-induced male reproductive damage in mice (2024-11-06)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.137241
影響因子 :7.7
引用產(chǎn)品: TM4 細(xì)胞
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Cadmium exposure induces pyroptosis of TM4 cells through oxidative stress damage and inflammasome activation (2024-01-06)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2024.115930
影響因子 :6.8
引用產(chǎn)品: TM4 細(xì)胞
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Compound probiotics regulate the NRF2 antioxidant pathway to inhibit aflatoxin B1-induced autophagy in mouse Sertoli TM4 cells (2024-06-25)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2024.116619
影響因子 :6.2
引用產(chǎn)品: TM4 細(xì)胞
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The Protective Effect of Quercetin against the Cytotoxicity Induced by Fumonisin B1 in Sertoli Cells (2024-08-12)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影響因子 :4.9
引用產(chǎn)品: TM4 細(xì)胞 , 特級胎牛血清 , 青霉素-鏈霉素溶液(雙抗),100× , 馬血清 , DMEM/F12 培養(yǎng)基
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Suppressed testicular macrophage M1 polarization by HDAC5 enforces insensitivity to LPS-elicited blood-testis barrier damage (2024-08-14)
期刊:FOOD AND CHEMICAL TOXICOLOGY
影響因子 :3.9
引用產(chǎn)品: TM4 細(xì)胞
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Schisandrin B alleviates testicular inflammation and Sertoli cell apoptosis via AR-JNK pathway (2024-08-08)
期刊:Scientific Reports
DOI:10.1038/s41598-024-69389-1
影響因子 :3.8
引用產(chǎn)品: DMEM/F12 培養(yǎng)基 , 青霉素-鏈霉素溶液(雙抗),100× , 特級胎牛血清 , 馬血清 , THP-1 細(xì)胞 , TM4 細(xì)胞
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Oligoasthenozoospermia is alleviated in a mouse model by [Gly14]-humanin-mediated attenuation of oxidative stress and ferroptosis (2024-10-22)
期刊:Andrology
影響因子 :3.2
引用產(chǎn)品: 特級胎牛血清 , GC-1 spg 細(xì)胞 , TM3 細(xì)胞 , TM4 細(xì)胞 , GC-2spd(ts) 細(xì)胞
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OCTN2 expression and function in the Sertoli cells of testes from patients with non-obstructive azoospermia (2024-12-05)
期刊:JOURNAL OF MOLECULAR HISTOLOGY
DOI:10.1007/s10735-024-10298-y
影響因子 :2.9
引用產(chǎn)品: TM4 細(xì)胞
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Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120 (2023-09-24)
期刊:CELL PROLIFERATION
影響因子 :8.5
引用產(chǎn)品: TM4 細(xì)胞
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Toxic effects of Tripterygium glycoside tablets on the reproductive system of male rats by metabolomics, cytotoxicity, and molecular docking (2023-04-10)
期刊:PHYTOMEDICINE
DOI:10.1016/j.phymed.2023.154813
影響因子 :7.9
引用產(chǎn)品: TM3細(xì)胞專用培養(yǎng)基 , TM4細(xì)胞專用培養(yǎng)基 , TM3 細(xì)胞 , TM4 細(xì)胞
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Puerarin improves busulfan-induced disruption of spermatogenesis by inhibiting MAPK pathways (2023-07-27)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2023.115231
影響因子 :7.5
引用產(chǎn)品: GC-1 spg 細(xì)胞 , TM4 細(xì)胞 , DMEM高糖 培養(yǎng)基 , 青霉素-鏈霉素溶液 (雙抗),100× , 0.25%胰蛋白酶溶液 (含酚紅,溶于D-Hank's)
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The role of ferroptosis mediated by Bmal1/Nrf2 in nicotine -induce injury of BTB integrity (2023-03-07)
期刊:FREE RADICAL BIOLOGY AND MEDICINE
DOI:10.1016/j.freeradbiomed.2023.02.024
影響因子 :7.4
引用產(chǎn)品: TM4 細(xì)胞
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DDX58 expression promotes inflammation and growth arrest in Sertoli cells by stabilizing p65 mRNA in patients with Sertoli cell-only syndrome (2023-03-22)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2023.1135753
影響因子 :7.3
引用產(chǎn)品: TM4 細(xì)胞 , TM4細(xì)胞專用培養(yǎng)基
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The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis (2023-07-20)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2023.115262
影響因子 :6.8
引用產(chǎn)品: TM4 細(xì)胞 , TM3 細(xì)胞 , GC-1 spg 細(xì)胞 , GC-2spd (ts) 細(xì)胞 , DMEM/F12 培養(yǎng)基 , DMEM高糖 培養(yǎng)基
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Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis (2023-11-15)
期刊:Frontiers in Cell and Developmental Biology
DOI:10.3389/fcell.2023.1282119
影響因子 :5.5
引用產(chǎn)品: TM4 細(xì)胞
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Non-Lethal Concentrations of CdCl2 Cause Marked Alternations in Cellular Stress Responses within Exposed Sertoli Cell Line (2023-02-09)
期刊:Toxics
影響因子 :4.6
引用產(chǎn)品: TM4 細(xì)胞
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A Probiotic Bacillus amyloliquefaciens D-1 Strain Is Responsible for Zearalenone Detoxifying in Coix Semen (2023-11-28)
期刊:Toxins
影響因子 :4.2
引用產(chǎn)品: TM4 細(xì)胞 , Hep G2 細(xì)胞
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Epigenetic programming of TBX2/CX43 mediates lower sperm quality in male offspring induced by prenatal dexamethasone exposure (2023-02-15)
期刊:TOXICOLOGICAL SCIENCES
影響因子 :3.8
引用產(chǎn)品: TM4 細(xì)胞
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Targeting APLN/APJ restores blood-testis barrier and improves spermatogenesis in murine and human diabetic models (2022-11-28)
期刊:Nature Communications
DOI:10.1038/s41467-022-34990-3
影響因子 :17.7
引用產(chǎn)品: TM4 細(xì)胞 , 293T [HEK-293T] 細(xì)胞
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Aucubin supplementation alleviate diabetes induced-disruption of blood-testis barrier and testicular damage via stabilizing cell junction integrity (2022-11-29)
期刊:EUROPEAN JOURNAL OF PHARMACOLOGY
DOI:10.1016/j.ejphar.2022.175430
影響因子 :5.2
引用產(chǎn)品: TM4 細(xì)胞
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Environmental cadmium impairs blood-testis barrier via activating HRI-responsive mitochondrial stress in mice (2021-12-09)
期刊:Science Of The Total Environment
DOI:10.1016/j.scitotenv.2021.152247
影響因子 :10.8
引用產(chǎn)品: TM4 細(xì)胞
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Human placental mesenchymal stem cells ameliorate chemotherapy-induced damage in the testis by reducing apoptosis/oxidative stress and promoting autophagy (2021-03-20)
期刊:Stem Cell Research & Therapy
DOI:10.1186/s13287-021-02275-z
影響因子 :8.1
引用產(chǎn)品: TM4 細(xì)胞
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Polypyrimidine tract?binding protein 1 regulates the Sertoli cell blood?testis barrier by promoting the expression of tight junction proteins (2021-06-07)
期刊:Experimental and Therapeutic Medicine
影響因子 :2.7
引用產(chǎn)品: TM4 細(xì)胞
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Aucubin, a natural iridoid glucoside, attenuates oxidative stress-induced testis injury by inhibiting JNK and CHOP activation via Nrf2 up-regulation (2019-07-27)
期刊:Phytomedicine
DOI:10.1016/j.phymed.2019.153057
影響因子 :7.9
引用產(chǎn)品: TM4 細(xì)胞
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Chemosensitization effect of cerium oxide nanosheets by suppressing drug detoxification and efflux (2018-10-18)
期刊:Ecotoxicology And Environmental Safety
DOI:10.1016/j.ecoenv.2018.10.013
影響因子 :6.8
引用產(chǎn)品: TM4 細(xì)胞
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