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hCMEC/D3 (人腦微血管內(nèi)皮細胞) (STR鑒定正確)
貨號：CL-0843
規(guī)格：1×10?cells/T25培養(yǎng)瓶
價格： ￥2000
生長培養(yǎng)基：hCMEC/D3 細胞專用培養(yǎng)基
產(chǎn)品概述
| 名稱 | hCMEC/D3 (人腦微血管內(nèi)皮細胞) (STR鑒定正確) |
| 別稱 | HCMEC/D3; CMEC/D3; human Cortical Microvessels Endothelial Cells/D3 |
| 種屬 | 人類 |
| 生長特性 | 貼壁細胞 |
| 細胞形態(tài) | 紡錘形細胞 |
| 凍存條件 | 凍存液：55% 基礎培養(yǎng)基+40% FBS+5% DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認） |
生長培養(yǎng)基:
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:6 |
| 推薦換液頻率 | 2-3次/周 |
| 背景描述 | The hCMEC/D3 cell line was derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. The primary isolate was enriched in cerebral endothelial cells (CECs). In the first passage, cells were sequentially immortalized by lentiviral vector transduction with the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen, following which CEC were selectively isolated by limited dilution cloning, and clones were extensively characterized for brain endothelial phenotype. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). |
| 年齡（性別） | 女性；成人 |
| 組織來源 | 腦微血管 |
| 細胞類型 | 轉(zhuǎn)化細胞系 |
| 生物安全等級 | BSL-2 |
| 保藏機構 | KCB; KCB 2019022YJ |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 10,12 D2S1338 16,20 D3S1358 16,17 D5S818 11 D7S820 10,12 D8S1179 15,16 D13S317 11,14 D16S539 9,12 D18S51 12,19 D19S433 12,15 D21S11 30,32.2 FGA 22,26 PentaD 10,12 PentaE 5,10 TH01 7,9 TPOX 10,11 vWA 14,17 D6S1043 17,18 D12S391 19.3,22 D2S441 13 -
STR鑒定圖
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參考文獻
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Drop-shaped microgrooves guide unidirectional cell migration for enhanced endothelialization (2025-02-24)
期刊：Nature Communications
DOI：10.1038/s41467-025-57146-5
影響因子 ：14.7
引用產(chǎn)品： hCMEC/D3 細胞
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A critical role for microglia in regulating metabolic homeostasis and neural repair after spinal cord injury (2024-10-15)
期刊：FREE RADICAL BIOLOGY AND MEDICINE
DOI：10.1016/j.freeradbiomed.2024.10.288
影響因子 ：7.1
引用產(chǎn)品： SH-SY5Y [SHSY-5Y] 細胞 , hCMEC/D3 細胞
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A label-free electrochemical biosensor based on graphene quantum dots-nanoporous gold nanocomposite for highly sensitive detection of glioma cell (2024-12-16)
期刊：ANALYTICA CHIMICA ACTA
影響因子 ：5.7
引用產(chǎn)品： U251 細胞 , NIH/3T3 細胞 , SCC7 細胞 , 4T1 細胞 , B16-F10 細胞 , Hela 細胞 , Hep G2 細胞 , LN229 [LN-229; LNT-229] 細胞 , PC-9 細胞 , LLC [LL/2; LLC1] 細胞 , hCMEC/D3 細胞 , HL-7702 [L-02; LO2] 細胞
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Reactive astrocytes promote tumor progression by up-regulating tumor protocadherin 1 expression in lung cancer brain metastasis (2024-07-20)
期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2024.150431
影響因子 ：2.5
引用產(chǎn)品： hCMEC/D3 細胞
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