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產(chǎn)品概述
名稱 | DC2.4 (小鼠骨髓來源樹突狀細(xì)胞) (STR鑒定正確) |
別稱 | DC 2.4 |
種屬 | 小鼠 |
生長特性 | 貼壁細(xì)胞,少量懸浮 |
細(xì)胞形態(tài) | 圓形;梭形,胞突呈分枝狀 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:4 |
推薦換液頻率 | 2-3天/次 |
注意事項(xiàng) | 圓形貼壁,少量貼壁細(xì)胞伸展開,少量懸浮。密度低時(shí)漂浮較少,密度高時(shí),漂浮比例增大,漂浮部分也可以培養(yǎng)。 |
背景描述 | DC2.4是通過用表達(dá)小鼠粒細(xì)胞-巨噬細(xì)胞-CSF(GM-CSF)和myc和raf致癌基因的逆轉(zhuǎn)錄病毒載體轉(zhuǎn)導(dǎo)C57BL/6小鼠的骨髓分離物而產(chǎn)生的永生化小鼠樹突狀細(xì)胞。DC2.4表現(xiàn)出樹突細(xì)胞的特征,包括細(xì)胞形態(tài)和樹突細(xì)胞特異性標(biāo)記物的表達(dá),以及吞噬MHC I類和II類分子并在其上呈遞外源性抗原的能力。 |
細(xì)胞類型 | 轉(zhuǎn)化細(xì)胞系 |
生物安全等級 | BSL-1 |
STR鑒定
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STR位點(diǎn)信息
18-3 15,16 6-7 17 5-5 17,18 X-1 28 1-2 19 7-1 26.2,27.2 8-1 16 1-1 17 19-2 13 15-3 22.3,23.3 12-1 17 6-4 18 4-2 20.3,21.3 3-2 14 2-1 16 13-1 17.1
11-2 16,17 17-2 16 -
STR鑒定圖
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參考文獻(xiàn)
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Nanoadjuvant-triggered STING activation evokes systemic immunotherapy for repetitive implant-related infections (2024-01-24)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2024.01.020
影響因子 :18.9
引用產(chǎn)品: DC2.4 細(xì)胞 , 小鼠骨髓樹突狀細(xì)胞 (未成熟DC細(xì)胞)
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Tailoring therapeutics via a systematic beneficial elements comparison between photosynthetic bacteria-derived OMVs and extruded nanovesicles (2024-02-26)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2024.02.025
影響因子 :18.9
引用產(chǎn)品: DC2.4 細(xì)胞
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An emerging antibacterial nanovaccine for enhanced chemotherapy by selectively eliminating tumor-colonizing bacteria (2024-06-14)
期刊:Science Bulletin
DOI:10.1016/j.scib.2024.06.016
影響因子 :18.8
引用產(chǎn)品: CT26.WT 細(xì)胞 , DC2.4 細(xì)胞
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Reprogramming exosomes for immunity-remodeled photodynamic therapy against non-small cell lung cancer (2024-05-21)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2024.05.030
影響因子 :18.0
引用產(chǎn)品: DC2.4 細(xì)胞 , B16-F10 細(xì)胞 , LLC [LL/2; LLC1] 細(xì)胞 , RAW 264.7 細(xì)胞
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Hypoxia Reversion and STING Pathway Activation through Large Mesoporous Nanozyme for Near-Infrared-II Light Amplified Tumor Polymetallic-Immunotherapy (2024-08-09)
期刊:ACS Nano
影響因子 :15.8
引用產(chǎn)品: DC2.4 細(xì)胞
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Cancer cell membrane proteins-encapsulated nanovaccine enhances cancer immunotherapy and prevention by provoking antigen-specific cellular immunity via the dendritic cell-targeted delivery (2024-01-06)
期刊:CHEMICAL ENGINEERING JOURNAL
影響因子 :15.1
引用產(chǎn)品: DC2.4 細(xì)胞 , 4T1 細(xì)胞
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A combination of a TLR7/8 agonist and an epigenetic inhibitor suppresses triple-negative breast cancer through triggering anti-tumor immune (2024-05-29)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-024-02525-1
影響因子 :10.6
引用產(chǎn)品: 特級胎牛血清 , RPMI-1640 培養(yǎng)基 , DC2.4 細(xì)胞
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Dual-Functional Nanodroplet for Tumor Vasculature Ultrasound Imaging and Tumor Immunosuppressive Microenvironment Remodeling (2024-07-19)
期刊:Advanced Healthcare Materials
影響因子 :10.0
引用產(chǎn)品: 人臍靜脈內(nèi)皮細(xì)胞 , Hela 細(xì)胞 , 4T1 細(xì)胞 , DC2.4 細(xì)胞
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Isoliquiritigenin alleviates SLC7A11-mediated efferocytosis inhibition to promote wounds healing in diabetes (2024-10-19)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2024.117578
影響因子 :6.9
引用產(chǎn)品: 人臍靜脈內(nèi)皮細(xì)胞 , DC2.4 細(xì)胞
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Novel mRNA adjuvant ImmunER enhances prostate cancer tumor-associated antigen mRNA therapy via augmenting T cell activity (2024-06-27)
期刊:OncoImmunology
DOI:10.1080/2162402X.2024.2373526
影響因子 :6.5
引用產(chǎn)品: DC2.4 細(xì)胞 , B16 細(xì)胞 , 293 [HEK-293] 細(xì)胞 , 293T [HEK-293T] 細(xì)胞 , TC-1 細(xì)胞
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Monocarboxylate transporter blockage-enabled lactate redistribution reshapes immunosuppressive tumor microenvironment and enhances chemodynamic immunotherapy (2023-11-07)
期刊:CHEMICAL ENGINEERING JOURNAL
影響因子 :15.1
引用產(chǎn)品: CT26.WT 細(xì)胞 , DC2.4 細(xì)胞
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Pre-Induced ICD Membrane-Coated Carrier-Free Nanoparticles for the Personalized Lung Cancer Immunotherapy (2023-03-18)
期刊:Small Methods
影響因子 :12.4
引用產(chǎn)品: DC2.4 細(xì)胞
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Intelligent Ti3C2–Pt heterojunction with oxygen self-supply for augmented chemo-sonodynamic/immune tumor therapy (2023-08-04)
期刊:Materials Today Nano
DOI:10.1016/j.mtnano.2023.100386
影響因子 :10.3
引用產(chǎn)品: DC2.4 細(xì)胞
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Endogenous/Exogenous Nanovaccines Synergistically Enhance Dendritic Cell-Mediated Tumor Immunotherapy (2023-02-18)
期刊:Advanced Healthcare Materials
影響因子 :10.0
引用產(chǎn)品: DC2.4 細(xì)胞 , B16 細(xì)胞
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Delivery of Mrna Vaccine with 1, 2-diesters-derived Lipids Elicits Fast Liver Clearance for Safe and Effective Cancer Immunotherapy. (2023-11-21)
期刊:Advanced Healthcare Materials
影響因子 :10.0
引用產(chǎn)品: B16 細(xì)胞 , RM-1 細(xì)胞 , DC2.4 細(xì)胞 , 293 [HEK-293] 細(xì)胞
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PTT/ PDT-induced microbial apoptosis and wound healing depend on immune activation and macrophage phenotype transformation (2023-06-25)
期刊:Acta Biomaterialia
DOI:10.1016/j.actbio.2023.06.025
影響因子 :9.7
引用產(chǎn)品: DC2.4 細(xì)胞 , NCTC clone 929 [L 細(xì)胞, L-929] 細(xì)胞
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Extracellular vesicles hybrid plasmid-loaded lipid nanovesicles for synergistic cancer immunotherapy (2023-10-27)
期刊:Materials Today Bio
DOI:10.1016/j.mtbio.2023.100845
影響因子 :8.2
引用產(chǎn)品: DC2.4 細(xì)胞
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Doxorubicin-Loaded Platelet Decoys for Enhanced Chemoimmunotherapy Against Triple-Negative Breast Cancer in Mice Model (2023-06-30)
期刊:International Journal of Nanomedicine
影響因子 :8.0
引用產(chǎn)品: 特級胎牛血清 , RPMI-1640 培養(yǎng)基 , DC2.4 細(xì)胞 , MDA-MB-231 細(xì)胞
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Cascade catalysis-coordinated nanorobot toward synergistic cancer chemoimmunotherapy (2023-08-30)
期刊:Journal of Materials Chemistry B
影響因子 :7.0
引用產(chǎn)品: 4T1 細(xì)胞 , DC2.4 細(xì)胞
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Interferon-γ regulates immunosuppression in septic mice by promoting the Warburg effect through the PI3K/AKT/mTOR pathway (2023-07-11)
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-023-00690-x
影響因子 :5.7
引用產(chǎn)品: DC2.4 細(xì)胞 , L-谷氨酰胺溶液 (200mM),100× , 青霉素-鏈霉素溶液 (雙抗),100× , MEM非必需氨基酸溶液 (NEAA),100× , β-巰基乙醇 (55mM),1000× , 特級胎牛血清
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Resveratrol-loaded macrophage exosomes alleviate multiple sclerosis through targeting microglia (2022-12-15)
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2022.12.026
影響因子 :11.5
引用產(chǎn)品: RAW 264.7 細(xì)胞 , DC2.4 細(xì)胞 , CTLL-2 細(xì)胞 , CTLL-2細(xì)胞專用培養(yǎng)基 , BV2 細(xì)胞 , U-87 MG 細(xì)胞
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Na2S2O4@Co-metal organic framework (ZIF-67) @glucose oxidase for biofilm-infecting wound healing with immune activation (2022-11-30)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2022.11.265
影響因子 :8.0
引用產(chǎn)品: 人臍靜脈內(nèi)皮細(xì)胞 , DC2.4 細(xì)胞
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Dendritic cells originating exosomal miR-193b-3p induces regulatory T cells to alleviate liver transplant rejection (2022-12-21)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2022.109541
影響因子 :5.7
引用產(chǎn)品: DC2.4 細(xì)胞
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Transcriptomic analysis reveal the responses of dendritic cells to VDBP (2022-03-12)
期刊:Genes & Genomics
DOI:10.1007/s13258-022-01234-z
影響因子 :2.1
引用產(chǎn)品: DC2.4 細(xì)胞
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