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產(chǎn)品概述
| 名稱 | Ca Ski (人宮頸癌腸轉(zhuǎn)移細(xì)胞/人宮頸癌上皮細(xì)胞) (STR鑒定正確) |
| 別稱 | Ca-Ski; CaSki; Caski; CASKI |
| 種屬 | 人 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 背景描述 | Ca Ski細(xì)胞是從小腸腸系膜轉(zhuǎn)移灶的細(xì)胞中建立的。據(jù)報(bào)道，Ca Ski細(xì)胞含有完整的HPV-16(每個細(xì)胞大約600個拷貝)和HPV-18相關(guān)序列。 |
| 年齡（性別） | 女性；40歲 |
| 組織來源 | 宮頸癌腸轉(zhuǎn)移 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 宮頸癌細(xì)胞 |
| 生物安全等級 | BSL-2 |
| 倍增時間 | ~60-80 hours |
| 基因表達(dá)情況 | beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen |
| 保藏機(jī)構(gòu) | ATCC; CRL-1550 ATCC; CRM-CRL-1550 ATCC; CRL-7915BCRC; 60251 ECACC; 87020501 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 10 D2S1338 21 D3S1358 15 D5S818 13 D7S820 8,11 D8S1179 15 D13S317 8,12 D16S539 11,12 D18S51 17 D19S433 15,16 D21S11 30 FGA 21 PentaD 10 PentaE 5 TH01 7 TPOX 8 vWA 17 D6S1043 11,14 D12S391 18 D2S441 11 -
STR鑒定圖
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參考文獻(xiàn)
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NSUN7 promotes cervical cancer progression through activation of ErbB signaling pathway (2025-02-15)
期刊：FUNCTIONAL & INTEGRATIVE GENOMICS
DOI：10.1007/s10142-025-01546-9
影響因子 ：3.9
引用產(chǎn)品： Ca Ski 細(xì)胞 , C-33 A 細(xì)胞
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N-acetyltransferase 10 Promotes Cervical Cancer Progression Via N4-acetylation of SLC7A5 mRNA (2025-02-20)
期刊：Frontiers in Bioscience-Landmark
影響因子 ：3.3
引用產(chǎn)品： SiHa 細(xì)胞 , Ca Ski 細(xì)胞 , 特級胎牛血清
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SOX2 promotes the glycolysis process to accelerate cervical cancer progression by regulating the expression of HK2 (2025-01-16)
期刊：ACTA HISTOCHEMICA
DOI：10.1016/j.acthis.2025.152230
影響因子 ：2.3
引用產(chǎn)品： Hela 細(xì)胞 , Ca Ski 細(xì)胞 , C-33 A 細(xì)胞 , SiHa 細(xì)胞 , DMEM高糖 培養(yǎng)基 , 特級胎牛血清 , 青霉素-鏈霉素溶液（雙抗），100×
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Co-freezing localized CRISPR-Cas12a system enables rapid and sensitive nucleic acid analysis (2024-10-05)
期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-024-02831-8
影響因子 ：10.6
引用產(chǎn)品： Hela 細(xì)胞 , A549 [A-549] 細(xì)胞 , NCI-H292 細(xì)胞 , Ca Ski 細(xì)胞 , 16HBE 細(xì)胞
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Function and mechanism of GBP1 in the development and progression of cervical cancer (2024-01-02)
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-023-04837-6
影響因子 ：7.4
引用產(chǎn)品： Ca Ski 細(xì)胞 , DMEM高糖 培養(yǎng)基
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Combining Mitomycin C with inhibition of BAD phosphorylation enhances apoptotic cell death in advanced cervical cancer (2024-08-24)
期刊：Translational Oncology
DOI：10.1016/j.tranon.2024.102103
影響因子 ：4.5
引用產(chǎn)品： SiHa 細(xì)胞 , C-33 A 細(xì)胞 , Ca Ski 細(xì)胞 , HT-3 細(xì)胞
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Oncoproteins E6 and E7 upregulate topoisomerase I to activate the cGAS-PD-L1 pathway in cervical cancer development (2024-08-02)
期刊：Frontiers in Pharmacology
DOI：10.3389/fphar.2024.1450875
影響因子 ：4.4
引用產(chǎn)品： SiHa 細(xì)胞 , Ca Ski 細(xì)胞 , Hela 細(xì)胞
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HIF-1A as a prognostic biomarker related to invasion, migration and immunosuppression of cervical cancer (2024-01-13)
期刊：Heliyon
DOI：10.1016/j.heliyon.2024.e24664
影響因子 ：4.0
引用產(chǎn)品： Hela 細(xì)胞 , Ca Ski 細(xì)胞 , 特級胎牛血清
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LIMK1 promotes the development of cervical cancer by up-regulating the ROS/Src-FAK/cofilin signaling pathway (2024-07-05)
期刊：Aging-US
影響因子 ：3.9
引用產(chǎn)品： SiHa 細(xì)胞 , Ca Ski 細(xì)胞
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APLN promotes the proliferation, migration, and glycolysis of cervical cancer through the PI3K/AKT/mTOR pathway (2024-03-30)
期刊：ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
影響因子 ：3.9
引用產(chǎn)品： C-33 A 細(xì)胞 , Hela 細(xì)胞 , SiHa 細(xì)胞 , Ca Ski 細(xì)胞 , ECT1/E6E7 細(xì)胞 , 人子宮頸上皮細(xì)胞完全培養(yǎng)基
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Serinc2 Drives the Progression of Cervical Cancer Through Regulating Myc Pathway (2024-10-17)
期刊：Cancer Medicine
影響因子 ：2.9
引用產(chǎn)品： SiHa 細(xì)胞 , Ca Ski 細(xì)胞 , C-33 A 細(xì)胞 , 青霉素-鏈霉素溶液（雙抗），100× , MEM 培養(yǎng)基
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N6-methyladenosine methylation on FSCN1 mediated by METTL14/IGF2BP3 contributes to human papillomavirus type 16-infected cervical squamous cell carcinoma (2024-04-28)
期刊：CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
影響因子 ：2.9
引用產(chǎn)品： Ca Ski 細(xì)胞 , C-33 A 細(xì)胞 , SiHa 細(xì)胞
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EPB41L3 Inhibits the Progression of Cervical Cancer Via the ERK/p38 MAPK Signaling Pathway (2024-06-21)
期刊：MOLECULAR BIOTECHNOLOGY
DOI：10.1007/s12033-024-01172-z
影響因子 ：2.4
引用產(chǎn)品： SiHa 細(xì)胞 , Hela 細(xì)胞 , Ca Ski 細(xì)胞 , 人角膜上皮細(xì)胞 , C-33 A 細(xì)胞
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Study on the Role of EPHB6 in Inhibiting the Malignant Progression of Cervical Cancer C33A Cells by Binding to CBX7 (2024-09-26)
期刊：CELL BIOCHEMISTRY AND BIOPHYSICS
DOI：10.1007/s12013-024-01458-8
影響因子 ：1.8
引用產(chǎn)品： Ca Ski 細(xì)胞
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PD-L1 Exon 3 Is a Hidden Switch of Its Expression and Function in Oral Cancer Cells (2023-05-03)
期刊：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影響因子 ：5.6
引用產(chǎn)品： FaDu 細(xì)胞 , U-2 OS [U2OS] 細(xì)胞 , Ca Ski 細(xì)胞
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An anti-PD-1 antisense oligonucleotide promotes the expression of soluble PD-1 by blocking the interaction between SRSF3 and an exonic splicing enhancer of PD-1 exon 3 (2023-12-04)
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.111280
影響因子 ：5.6
引用產(chǎn)品： MOLT-4 細(xì)胞 , FaDu 細(xì)胞 , Ca Ski 細(xì)胞 , HCT 116 細(xì)胞
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IGF2BP3-mediated regulation of GLS and GLUD1 gene expression promotes treg-induced immune escape in human cervical cancer (2023-11-15)
期刊：American Journal of Cancer Research
DOI：PMID:38058838
影響因子 ：5.3
引用產(chǎn)品： Hela 細(xì)胞 , Ca Ski 細(xì)胞
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HPV E6 promotes cell proliferation of cervical cancer cell by accelerating accumulation of RBM15 dependently of autophagy inhibition (2023-05-16)
期刊：CELL BIOLOGY INTERNATIONAL
影響因子 ：3.9
引用產(chǎn)品： C-33 A 細(xì)胞 , Ca Ski 細(xì)胞 , SiHa 細(xì)胞 , Hela 細(xì)胞 , MEM (含NEAA) 培養(yǎng)基 , 特級胎牛血清 , 青霉素-鏈霉素溶液 (雙抗)，100× , RPMI-1640 培養(yǎng)基 , DMEM高糖 培養(yǎng)基 , McCoy's 5A 培養(yǎng)基 , DMEM/F12 培養(yǎng)基
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Characterization of the microenvironment in different immune-metabolism subtypes of cervical cancer with prognostic significance (2023-02-03)
期刊：Frontiers in Genetics
DOI：10.3389/fgene.2023.1067666
影響因子 ：3.7
引用產(chǎn)品： Hela 細(xì)胞 , SiHa 細(xì)胞 , Ca Ski 細(xì)胞 , C-33 A 細(xì)胞
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Circ_0005615 promotes cervical cancer cell growth and metastasis by modulating the miR-138-5p/KDM2A axis (2023-07-02)
期刊：JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
影響因子 ：3.6
引用產(chǎn)品： SiHa 細(xì)胞 , Ca Ski 細(xì)胞 , 293T [HEK-293T] 細(xì)胞 , MEM 培養(yǎng)基 , RPMI-1640 培養(yǎng)基
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LINC00460 knockdown sensitizes cervical cancer to cisplatin by downregulating TGFBI (2023-12-28)
期刊：Chemical Biology & Drug Design
影響因子 ：3.0
引用產(chǎn)品： SiHa 細(xì)胞 , ME-180 細(xì)胞 , Hela 細(xì)胞 , Ca Ski 細(xì)胞
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Asperuloside Promotes Apoptosis of Cervical Cancer Cells through Endoplasmic Reticulum Stress-Mitochondrial Pathway (2023-04-20)
期刊：Chinese Journal of Integrative Medicine
影響因子 ：2.9
引用產(chǎn)品： Hela 細(xì)胞 , Ca Ski 細(xì)胞
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SHCBP1 contributes to the proliferation and self?renewal of cervical cancer cells and activation of the NF?κB signaling pathway through EIF5A (2023-04-21)
期刊：Oncology Letters
影響因子 ：2.9
引用產(chǎn)品： SiHa 細(xì)胞 , C-33 A 細(xì)胞 , Ca Ski 細(xì)胞 , DMEM/F12 培養(yǎng)基
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Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer (2023-09-16)
期刊：FUNCTIONAL & INTEGRATIVE GENOMICS
DOI：10.1007/s10142-023-01218-6
影響因子 ：2.9
引用產(chǎn)品： C-33 A 細(xì)胞 , Ca Ski 細(xì)胞 , Hela S3 細(xì)胞 , SiHa 細(xì)胞 , 人子宮頸上皮細(xì)胞
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BTG2 suppresses the growth and metastasis of cervical squamous cell carcinoma (2023-05-26)
期刊：PATHOLOGY RESEARCH AND PRACTICE
影響因子 ：2.8
引用產(chǎn)品： SiHa 細(xì)胞 , Ca Ski 細(xì)胞 , C-33 A 細(xì)胞
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TRIM21 promotes tumor progression and cancer stemness in cervical squamous cell carcinoma (2023-07-23)
期刊：PATHOLOGY RESEARCH AND PRACTICE
影響因子 ：2.8
引用產(chǎn)品： Ca Ski 細(xì)胞 , C-33 A 細(xì)胞 , SiHa 細(xì)胞
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Circular RNA circ_0000119 promotes cervical cancer cell growth and migration via miR-433-3p/PAK2 axis (2023-08-04)
期刊：JOURNAL OF APPLIED GENETICS
DOI：10.1007/s13353-023-00772-w
影響因子 ：2.4
引用產(chǎn)品： Ca Ski 細(xì)胞 , SiHa 細(xì)胞 , Ect1/E6E7 細(xì)胞
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Cellular Retinoic Acid Binding Protein 2 (CRABP2), Up-regulated by HPV E6/E7, Leads to Aberrant Activation of the Integrin β1/FAK/ERK Signaling Pathway and Aggravates the Malignant Phenotypes of Cervical Cancer (2023-11-24)
期刊：BIOCHEMICAL GENETICS
DOI：10.1007/s10528-023-10568-6
影響因子 ：2.4
引用產(chǎn)品： SiHa 細(xì)胞 , Ca Ski 細(xì)胞 , C-33 A 細(xì)胞 , Hela 細(xì)胞 , Hacat 細(xì)胞
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PLAC8 contributes to the malignant behaviors of cervical cancer cells by activating the SOX4-mediated AKT pathway (2023-01-05)
期刊：HISTOCHEMISTRY AND CELL BIOLOGY
DOI：10.1007/s00418-022-02175-0
影響因子 ：2.3
引用產(chǎn)品： SiHa 細(xì)胞 , C-33 A 細(xì)胞 , Ca Ski 細(xì)胞
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DDX49 Promotes Proliferation and Metastasis of Cervical Cancer by Regulation of AKT and Wnt/β-Catenin Signalings (2023-03-01)
期刊：ANNALS OF CLINICAL AND LABORATORY SCIENCE
DOI：PMID:37094865
影響因子 ：0.8
引用產(chǎn)品： Ca Ski 細(xì)胞 , Hela 細(xì)胞 , SiHa 細(xì)胞 , C-33 A 細(xì)胞 , 人子宮頸上皮細(xì)胞
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CircCCNB1 Knockdown Blocks the Progression of Cervical Cancer by Acting as Competing Endogenous RNA in the miR-370-3p/SOX4 Pathway (2023-01-01)
期刊：ANNALS OF CLINICAL AND LABORATORY SCIENCE
DOI：PMID:36889763
影響因子 ：0.8
引用產(chǎn)品： MS751 細(xì)胞 , Ca Ski 細(xì)胞 , SiHa 細(xì)胞 , C-33 A 細(xì)胞 , Hela 細(xì)胞 , MEM 培養(yǎng)基 , RPMI-1640 培養(yǎng)基
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Rapid generation of genetically engineered T cells for the treatment of virus‐related cancers (2022-09-09)
期刊：CANCER SCIENCE
影響因子 ：6.5
引用產(chǎn)品： Ca Ski 細(xì)胞
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CDK6 increases glycolysis and suppresses autophagy by mTORC1-HK2 pathway activation in cervical cancer cells (2022-02-15)
期刊：Cell Cycle
DOI：10.1080/15384101.2022.2039981
影響因子 ：5.2
引用產(chǎn)品： SiHa 細(xì)胞 , Ca Ski 細(xì)胞
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Signature of seven cuproptosis-related lncRNAs as a novel biomarker to predict prognosis and therapeutic response in cervical cancer (2022-09-20)
期刊：Frontiers in Genetics
影響因子 ：4.8
引用產(chǎn)品： Hela 細(xì)胞 , C-33 A 細(xì)胞 , Ca Ski 細(xì)胞
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OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway (2022-09-29)
期刊：ONCOLOGY REPORTS
影響因子 ：4.1
引用產(chǎn)品： C-33 A 細(xì)胞 , SiHa 細(xì)胞 , Ca Ski 細(xì)胞 , ME-180 細(xì)胞
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ZIC5 promotes aggressiveness and cancer stemness in cervical squamous cell carcinoma (2022-12-05)
期刊：PATHOLOGY RESEARCH AND PRACTICE
影響因子 ：3.3
引用產(chǎn)品： SiHa 細(xì)胞 , Ca Ski 細(xì)胞 , C-33 A 細(xì)胞
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FBXO39 predicts poor prognosis and correlates with tumor progression in cervical squamous cell carcinoma (2022-08-28)
期刊：PATHOLOGY RESEARCH AND PRACTICE
影響因子 ：3.3
引用產(chǎn)品： Ca Ski 細(xì)胞 , C-33 A 細(xì)胞 , SiHa 細(xì)胞
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tRNA?derived fragment tRF?Glu49 inhibits cell proliferation, migration and invasion in cervical cancer by targeting FGL1 (2022-08-09)
期刊：Oncology Letters
影響因子 ：3.1
引用產(chǎn)品： Ca Ski 細(xì)胞 , C-33 A 細(xì)胞 , SiHa 細(xì)胞 , Hela 細(xì)胞 , Hacat 細(xì)胞
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hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR-217 to upregulate anterior gradient 2 (2022-03-11)
期刊：Journal Of Clinical Laboratory Analysis
影響因子 ：3.1
引用產(chǎn)品： Ca Ski 細(xì)胞 , Hela 細(xì)胞 , C-33 A 細(xì)胞 , SiHa 細(xì)胞
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Circ_0042986 Presence Restrains Cervical Cancer Development via Upregulating PEG3 by Directly Targeting miR-582-3p (2022-08-26)
期刊：Reproductive Sciences
DOI：10.1007/s43032-022-01053-3
影響因子 ：2.9
引用產(chǎn)品： Hela 細(xì)胞 , Ca Ski 細(xì)胞 , HUVECs cell
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Circular RNA circVPRBP serves as a microRNA-106b-5p sponge to regulate proliferation and metastasis of cervical cancer cells via tripartite motif-containing protein 3 (2022-08-10)
期刊：ANTI-CANCER DRUGS
DOI：10.1097/CAD.0000000000001335
影響因子 ：2.4
引用產(chǎn)品： Ca Ski 細(xì)胞 , SiHa 細(xì)胞 , SiHa細(xì)胞專用培養(yǎng)基
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LncRNA Opa interacting protein 5-antisense RNA 1 (OIP5-AS1) promotes the migration, invasion and epithelial-mesenchymal transition (EMT) through targeting miR-147a/insulin-like growth factor 1 receptor (IGF1R) pathway in cervical cancer tissues and c (2022-03-01)
期刊：Journal Of Obstetrics And Gynaecology Research
影響因子 ：1.6
引用產(chǎn)品： Hela 細(xì)胞 , Ca Ski 細(xì)胞 , SiHa 細(xì)胞 , ME-180 細(xì)胞
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Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway (2021-06-27)
期刊：Journal Of Cellular And Molecular Medicine
影響因子 ：5.3
引用產(chǎn)品： Ca Ski 細(xì)胞 , ME-180 細(xì)胞
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T-box transcription factor TBX1, targeted by microRNA-6727-5p, inhibits cell growth and enhances cisplatin chemosensitivity of cervical cancer cells through AKT and MAPK pathways (2021-02-08)
期刊：Bioengineered
DOI：10.1080/21655979.2021.1880732
影響因子 ：4.9
引用產(chǎn)品： Ca Ski 細(xì)胞 , RPMI-1640 培養(yǎng)基 , SiHa 細(xì)胞 , MEM (含NEAA) 培養(yǎng)基
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Vitamin E succinate exerts anti-tumour effects on human cervical cancer cells via the CD47-SIRPɑ pathway both in vivo and in vitro (2021-05-05)
期刊：Journal of Cancer
影響因子 ：3.9
引用產(chǎn)品： Ca Ski 細(xì)胞
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ASF1B promotes cervical cancer progression through stabilization of CDK9 (2020-08-26)
期刊：Cell Death & Disease
DOI：10.1038/s41419-020-02872-5
影響因子 ：9.0
引用產(chǎn)品： Ca Ski 細(xì)胞 , Hela 細(xì)胞
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KMT2A regulates cervical cancer cell growth through targeting VDAC1. (2020-05-21)
期刊：Aging-US
影響因子 ：5.2
引用產(chǎn)品： SiHa 細(xì)胞 , Ca Ski 細(xì)胞
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Long non?coding RNA AK001903 regulates tumor progression in cervical cancer (2020-11-30)
期刊：Oncology Letters
影響因子 ：2.9
引用產(chǎn)品： Ca Ski 細(xì)胞 , Hela 細(xì)胞 , SiHa 細(xì)胞 , C-33 A 細(xì)胞
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MiRNA-211 triggers an autophagy-dependent apoptosis in cervical cancer cells: regulation of Bcl-2 (2019-10-21)
期刊：Naunyn-Schmiedebergs Archives of Pharmacology
DOI：10.1007/s00210-019-01720-4
影響因子 ：3.6
引用產(chǎn)品： Hela 細(xì)胞 , Ca Ski 細(xì)胞
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Inhibition of miR-574-5p suppresses cell growth and metastasis and enhances chemosensitivity by targeting RNA binding protein QKI in cervical cancer cells (2019-11-30)
期刊：Naunyn-Schmiedebergs Archives of Pharmacology
DOI：10.1007/s00210-019-01772-6
影響因子 ：3.6
引用產(chǎn)品： C-33 A 細(xì)胞 , SiHa 細(xì)胞 , Hela 細(xì)胞 , Ca Ski 細(xì)胞
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