CD253/TNFSF10/TRAIL, Human, Recombinant
英文名稱：CD253/TNFSF10/TRAIL, Human, Recombinant
貨號(hào)： PCK219
價(jià)格： ￥420 ￥1080 ￥4880 ￥6680
規(guī)格：
10μg
50μg
500μg
1mg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 液體 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Tumor Necrosis Factor Ligand Superfamily Member 10;Apo-2 Ligand;Apo-2L;TNF-Related Apoptosis-Inducing Ligand;Protein TRAIL;CD253;TNFSF10;APO2L;TRAIL |
| 物種 | 人 |
| 表達(dá)宿主 | E.coli |
| 序列信息 | Arg115-Gly281 |
| 檢索號(hào) | P50591 |
| 分子量 | 19.5 kDa |
| 表觀分子量 | 16 kDa |
| 生物活性 | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 1-10 ng/mL. |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲(chǔ)存條件 | Store at ≤-70℃, stable for 6 months after receipt. Store at ≤-70℃, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 運(yùn)輸條件 | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
| 有效期 | 6個(gè)月 |
| 制劑 | Supplied as a 0.2 μm filtered solution of 40 mM Tris-HCl, 300 mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween80, 10% Glycerol, pH 7.0. |
| 背景介紹 | Human TNFSF10 is a type II transmembrane Protein with an intracellular N-terminus and a‘TNF homology domain' (THD) at the extracellular C terminus. TNFSF10 can interact with several distinct Receptors. Two of these Receptors that belongs to TNFR superfamily, DR4 (TRAIL-R1) and DR5 (TRAIL-R2/TRICK2), are plasma membrane Proteins containing intracellular death domains essential for activating apoptosis. TNFSF10 is promising for cancer therapy because it is cytotoxic and activates apoptosis in the majority of malignant cells, but not in normal cells. |
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么？
我們公司的因子都是重組的，是不含動(dòng)物源成分的。
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Q2:細(xì)胞因子用無(wú)菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時(shí)，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過(guò)PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長(zhǎng)時(shí)間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無(wú)菌水溶解后需要再次離心嗎？
加了無(wú)菌水后，如果沒(méi)有掛壁現(xiàn)象是不用離心的，如果您放置了一段時(shí)間，再次使用時(shí)是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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