IL-6/IFN-β2/BSF-2, Human, Recombinant
英文名稱：IL-6/IFN-β2/BSF-2, Human, Recombinant
貨號： PCK085
價格： ￥9680 ￥2780 ￥880 ￥13880
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 500μg / 50μg / 10μg / 1mg |
| 別名 | IFN-β2;B-Cell Differentiation Factor (BCDF);BSF-2;HPGF;HSF;MGI-2 |
| 物種 | 人 |
| 表達(dá)宿主 | E.coli |
| 序列信息 | MVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM with polyhistidine tag at the C-terminus. |
| 檢索號 | P05231.1 |
| 分子量 | 21.8 kDa |
| 表觀分子量 | 22 kDa |
| 標(biāo)簽 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is < 0.5 ng/mL. The specific activity of recombinant human IL-6 is approximately > 5 × 10^8 IU/mg.Measure by its ability to induce proliferation in MCF-7 cells. The ED50 for this effect is < 4.4 ng/mL. |
| 純度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 內(nèi)毒素 | < 0.1 EU per 1 μg of the protein by the LAL method. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 背景介紹 | Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6's role as an anti-inflammatory myokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. |
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期刊：HUMAN & EXPERIMENTAL TOXICOLOGY
影響因子：2.7
引用產(chǎn)品： GM-CSF/CSF2 (C-6His), Mouse, Recombinant, 特級胎牛血清, IL-6/IFN-β2/BSF-2, Human, Recombinant, 丙酮酸鈉溶液（100 mM），100×
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期刊：JOURNAL OF CLINICAL LABORATORY ANALYSIS
影響因子：3.124
引用產(chǎn)品： SCF/c-Kit/KIT ligand, Human, Recombinant, IL-6/IFN-β2/BSF-2, Human, Recombinant, 293T [HEK-293T] 細(xì)胞, 人皮膚肥大細(xì)胞完全培養(yǎng)基
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Q1:因子到貨了，里面有兩管溶液，一個是水，一個是海藻糖溶液，用哪一個稀釋呢？
1、將裝有細(xì)胞因子的管子放入小離心機內(nèi)短暫離心20~30s（離心機最大轉(zhuǎn)速即可），使附著在管壁的微量蛋白聚集在管底； 2、先用無菌水溶解因子，很少量水即可溶解因子，注意計算溶解后濃度不低于100 μg/mL； 3、用贈送的含海藻糖的PBS溶液進一步稀釋到您實驗合適的濃度即可，然后分裝后放入-20℃以下保藏； 4、因子復(fù)融重懸過程應(yīng)輕柔，不可以震蕩渦旋。
識別碼示意圖
