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產(chǎn)品概述
| 名稱(chēng) | OE19 (人食管癌細(xì)胞) (STR鑒定正確) |
| 別稱(chēng) | OE-19; JROECL 19; JROECL19; OEC19 |
| 種屬 | 人 |
| 生長(zhǎng)特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:2-1:4 |
| 推薦換液頻率 | 2-3天/次 |
| 背景描述 | OE19, also known as JROECL19, was originally one of five permanent human oesophageal adenocarcinoma (EAC) cell lines established in 1993. It is from a stage III tumour of a 72 year old male patient1 and deposited in ECACC by Drs J C Rockett/A Morris, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital. Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines but large cell lung cancer cell line H460, colorectal adenocarcinoma cell line SW620, and gastric fundus carcinoma cell line SKGT-2, respectively. However, they confirmed that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs2 . All nine of these verified EAC cell lines, together with their genotyping information, have been deposited with ECACC. |
| 年齡（性別） | 男性；72歲 |
| 組織來(lái)源 | 食管癌 |
| 細(xì)胞類(lèi)型 | 腫瘤細(xì)胞 |
| 腫瘤類(lèi)型 | 食管癌細(xì)胞 |
| 生物安全等級(jí) | BSL-1 |
| 倍增時(shí)間 | ~50-60 hours (DSMZ=ACC-700) |
| 致瘤性 | 裸小鼠致癌 |
| 保藏機(jī)構(gòu) | ECACC; 96071721 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 11,13 D2S1338 18,19 D3S1358 15,18 D5S818 11,14 D7S820 8 D8S1179 13,15 D13S317 9,11 D16S539 12,13 D18S51 12 D19S433 13 D21S11 30 FGA 23,26 PentaD 9 PentaE 5,8 TH01 8,9 TPOX 8 vWA 16,17,18 D6S1043 19 D12S391 20 D2S441 14,15 -
STR鑒定圖
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參考文獻(xiàn)
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USP14 inhibition by degrasyn induces YAP1 degradation and suppresses the progression of radioresistant esophageal cancer (2024-12-14)
期刊：NEOPLASIA
影響因子 ：6.3
引用產(chǎn)品： TE-12 細(xì)胞 , OE19 細(xì)胞 , 青霉素-鏈霉素溶液（雙抗），100×
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COL8A1 Regulates Esophageal Squamous Carcinoma Proliferation and Invasion Through PI3K/AKT Pathway (2024-03-01)
期刊：ANNALS OF SURGICAL ONCOLOGY
DOI：10.1245/s10434-023-14370-x
影響因子 ：3.7
引用產(chǎn)品： OE19 細(xì)胞 , TE-10 細(xì)胞 , TE-1 細(xì)胞 , KYSE-510 細(xì)胞
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Tissue-derived exosome proteomics identifies promising diagnostic biomarkers for esophageal cancer (2023-11-15)
期刊：eLife
影響因子 ：7.7
引用產(chǎn)品： OE19 細(xì)胞 , KYSE-510 細(xì)胞
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