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C3H/10T1/2, Clone 8 (小鼠胚胎成纖維細(xì)胞) (STR鑒定正確)
貨號(hào):CL-0325
規(guī)格:1×10?cells/T25培養(yǎng)瓶
價(jià)格: ¥1800
生長(zhǎng)培養(yǎng)基:MEM(含NEAA)+10% FBS+1% P/S
產(chǎn)品概述
名稱 | C3H/10T1/2, Clone 8 (小鼠胚胎成纖維細(xì)胞) (STR鑒定正確) |
別稱 | C3H/10T1/2-clone8; C3H/10T1/2 CL8; C3H10T1/2CL8; 10T1/2; C3H10T1-2; C3H10T1/2; C3H-10T1/2; C3H 10T1/2; C3H/10T1/2 |
種屬 | 小鼠 |
生長(zhǎng)特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 成纖維細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
生長(zhǎng)培養(yǎng)基:
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:4 |
推薦換液頻率 | 2-3次/周 |
背景描述 | C3H/10T1/2, Clone 8細(xì)胞是由C·Reznikoff等人于1972從C3H系小鼠胚胎細(xì)胞分離。C3H/10T1/2, Clone 8細(xì)胞對(duì)過度長(zhǎng)滿的細(xì)胞有絲分裂抑制非常敏感,在同源小鼠中不產(chǎn)生腫瘤,無自然轉(zhuǎn)化的背景;C3H/10T1/2, Clone 8細(xì)胞也不含明顯內(nèi)源性轉(zhuǎn)化鼠類白血病或肉瘤病毒。 |
年齡(性別) | 胚胎 |
組織來源 | 胚胎 |
細(xì)胞類型 | 自發(fā)永生化細(xì)胞 |
生物安全等級(jí) | BSL-1 |
致瘤性 | No, in immunosuppressed mice.Yes, in semisolid medium. |
保藏機(jī)構(gòu) | ATCC; CCL-226 ECACC; 99072801 |
STR鑒定
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STR位點(diǎn)信息
18-3 16 6-7 12 5-5 15 X-1 26 1-2 16 7-1 26 8-1 15 1-1 10 19-2 12 15-3 25.3 12-1 16 6-4 18 4-2 20.3 3-2 14 2-1 9 13-1 17.1
11-2 16 17-2 14 -
STR鑒定圖
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參考文獻(xiàn)
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Compressible, anti-fatigue, extreme environment adaptable, and biocompatible supramolecular organohydrogel enabled by lignosulfonate triggered noncovalent network (2025-01-02)
期刊:Nature Communications
DOI:10.1038/s41467-024-55530-1
影響因子 :14.7
引用產(chǎn)品: ATDC-5 細(xì)胞 , C3H/10T1/2, Clone 8 細(xì)胞 , NIH/3T3 細(xì)胞
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MSCs-EVs?harboring OA?immune memory reprogram macrophage phenotype via modulation of the mt-ND3/NADH-CoQ axis for OA treatment (2025-02-25)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-025-03216-1
影響因子 :10.6
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞 , RAW 264.7 細(xì)胞
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Isoliquiritigenin Ameliorates High-Fat Diet-Induced Obesity in Mice by Activating Brown Adipose Tissue (2025-02-14)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影響因子 :4.9
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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Effects of ADRM1 on osteoblast differentiation and mineralization in osteoporosis (2025-01-15)
期刊:American Journal of Translational Research
影響因子 :1.7
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞 , MC3T3-E1 Subclone 24 細(xì)胞
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Generation of iPSC-derived human venous endothelial cells for the modeling of vascular malformations and drug discovery (2024-11-22)
期刊:Cell Stem Cell
DOI:10.1016/j.stem.2024.10.015
影響因子 :19.8
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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Lesion-localized hydrogels functionalized with engineered extracellular matrix restore cellular homeostasis by enhancing mitochondrial energy metabolism for osteoarthritis therapy (2024-09-13)
期刊:CHEMICAL ENGINEERING JOURNAL
影響因子 :13.3
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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Localized Hydrogel Microspheres for Osteoarthritis Treatment: Recruitment and Differentiation of Stem Cells (2024-11-28)
期刊:Advanced Healthcare Materials
影響因子 :10.0
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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RNA-binding protein YBX3 promotes PPARγ-SLC3A2 mediated BCAA metabolism fueling brown adipogenesis and thermogenesis (2024-10-29)
期刊:Molecular Metabolism
DOI:10.1016/j.molmet.2024.102053
影響因子 :7.0
引用產(chǎn)品: 293T [HEK-293T] 細(xì)胞 , C3H/10T1/2, Clone 8 細(xì)胞
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EEF1B2 regulates bone marrow-derived mesenchymal stem cells bone-fat balance via Wnt/β-catenin signaling (2024-06-15)
期刊:CELLULAR AND MOLECULAR LIFE SCIENCES
DOI:10.1007/s00018-024-05297-x
影響因子 :6.2
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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The SIRT5-Mediated Upregulation of C/EBPβ Promotes White Adipose Tissue Browning by Enhancing UCP1 Signaling (2024-09-29)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影響因子 :4.9
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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Role and mechanism of histone demethylase PHF8 in weightlessness osteoporosis (2024-10-09)
期刊:EXPERIMENTAL CELL RESEARCH
DOI:10.1016/j.yexcr.2024.114270
影響因子 :3.3
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain (2023-09-12)
期刊:Cell Metabolism
DOI:10.1016/j.cmet.2023.08.009
影響因子 :29.0
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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Senescent immune cells accumulation promotes brown adipose tissue dysfunction during aging (2023-06-02)
期刊:Nature Communications
DOI:10.1038/s41467-023-38842-6
影響因子 :16.6
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞 , PC-12 (Low differentiation) 細(xì)胞
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Regulation of pericyte metabolic reprogramming restricts the AKI to CKD transition (2023-05-23)
期刊:METABOLISM-CLINICAL AND EXPERIMENTAL
DOI:10.1016/j.metabol.2023.155592
影響因子 :9.8
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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Prussian Blue Nanoparticle-Entrapped GelMA Gels Laden with Mesenchymal Stem Cells as Prospective Biomaterials for Pelvic Floor Tissue Repair (2023-01-31)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影響因子 :5.6
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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The histone methyltransferase DOT1L is a new epigenetic regulator of pulmonary fibrosis (2022-01-17)
期刊:Cell Death & Disease
DOI:10.1038/s41419-021-04365-5
影響因子 :9.0
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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Olanzapine induces weight gain in offspring of prenatally exposed poly I:C rats by reducing brown fat thermogenic activity (2022-09-30)
期刊:Frontiers in Pharmacology
DOI:10.3389/fphar.2022.1001919
影響因子 :6.0
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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A Quartet Network Analysis Identifying Mechanically Responsive Long Noncoding RNAs in Bone Remodeling (2022-03-09)
期刊:Frontiers in Bioengineering and Biotechnology
影響因子 :5.7
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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Role of Yes-associated protein (YAP) in regulation of mesenchymal stem cell tenogenic differentiation (2022-01-19)
期刊:Journal Of Molecular Histology
DOI:10.1007/s10735-022-10059-9
影響因子 :3.2
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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Stem Cell-Recruiting Injectable Microgels for Repairing Osteoarthritis (2021-07-17)
期刊:Advanced Functional Materials
影響因子 :19.0
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞
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miR-199b-5p promoted chondrogenic differentiation of C3H10T1/2 cells by regulating JAG1 (2020-09-01)
期刊:Journal of Tissue Engineering and Regenerative Medicine
影響因子 :3.3
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞 , MEM 培養(yǎng)基
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miR‐199b‐5p promoted chondrogenic differentiation of C3H10T1/2 cells by regulating JAG1 (2020-09-01)
期刊:Journal of Tissue Engineering and Regenerative Medicine
影響因子 :3.3
引用產(chǎn)品: C3H/10T1/2, Clone 8 細(xì)胞 , MEM (含NEAA) 培養(yǎng)基
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