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產(chǎn)品概述
名稱 | CFPAC-1 (人胰腺癌細(xì)胞) (STR鑒定正確) |
別稱 | CFPac-1; CF PAC-1; CF-PAC1; CF-Pac1; CF Pac1; CFPAC1; CFPac1; CFPAC |
種屬 | 人 |
生長特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:4 |
推薦換液頻率 | 2-3次/周 |
背景描述 | CFPAC-1細(xì)胞是胰腺管腺癌細(xì)胞系,建自26歲白人男性囊性纖維變性(CF)的肝轉(zhuǎn)移灶。CFPAC-1細(xì)胞表達(dá)囊性纖維變性跨膜調(diào)節(jié)因子(CFTR),CFPAC-1細(xì)胞形態(tài)為上皮細(xì)胞樣且頂端微絨毛極化。CFPAC-1細(xì)胞表達(dá)胰腺管細(xì)胞特征性的細(xì)胞角蛋白和癌胚抗原,倍增時間為30-32小時。CFPAC-1細(xì)胞是亞3倍體的細(xì)胞系,36%的細(xì)胞的染色體模式數(shù)為75。CFPAC-1細(xì)胞傳代至24代時,可在免疫抑制小鼠中致瘤。 |
年齡(性別) | 男性;26歲 |
組織來源 | 胰腺管腺癌,肝轉(zhuǎn)移 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 胰腺癌細(xì)胞 |
生物安全等級 | BSL-1 |
倍增時間 | ~30-45 hours |
致瘤性 | Yes, in nude mice (passage 34) |
抗原表達(dá)情況 | CA19-9 antigen, 12000 units/mL, epithelial keratins |
基因表達(dá)情況 | carcinoembryonic antigen (CEA), 9ng/mL; pancreatic oncofetal antigen (POA), 28ng/mL; adenocarcinoma associated antigen (ACAA), 5000ng/mL; CA 19-9 antigen, 12000 units/mL; epithelial keratins |
保藏機構(gòu) | ATCC; CRL-1918 ECACC; 91112501 |
STR鑒定
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STR位點信息
Amelogenin X,Y CSF1PO 10 D2S1338 18,23 D3S1358 16 D5S818 10,11 D7S820 8,10 D8S1179 11,15 D13S317 12 D16S539 9,11 D18S51 12 D19S433 13,15 D21S11 30,31.2 FGA 21,22 PentaD 11,13 PentaE 10,12 TH01 8 TPOX 8 vWA 17 D6S1043 20 D12S391 17 D2S441 10,14 -
STR鑒定圖
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參考文獻(xiàn)
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FOXM1-Driven CKS1B Upregulation Promotes Pancreatic Cancer Progression and Therapeutic Resistance (2025-01-13)
期刊:International Journal of Biological Sciences
影響因子 :8.2
引用產(chǎn)品: BxPC-3 細(xì)胞 , CFPAC-1 細(xì)胞 , MIA PaCa-2 細(xì)胞 , PANC-1 細(xì)胞 , Panc02 細(xì)胞 , PL45 細(xì)胞
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Concurrent inhibition of pBADS99 synergistically improves MEK inhibitor efficacy in KRASG12D-mutant pancreatic ductal adenocarcinoma (2024-02-26)
期刊:Cell Death & Disease
DOI:10.1038/s41419-024-06551-7
影響因子 :9.0
引用產(chǎn)品: SW 1990 細(xì)胞 , PANC-1 細(xì)胞 , AsPC-1 細(xì)胞 , CFPAC-1 細(xì)胞
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RHOF promotes Snail1 lactylation by enhancing PKM2-mediated glycolysis to induce pancreatic cancer cell endothelial–mesenchymal transition (2024-10-26)
期刊:Cancer & Metabolism
DOI:10.1186/s40170-024-00362-2
影響因子 :6.0
引用產(chǎn)品: CFPAC-1 細(xì)胞 , DMEM高糖 培養(yǎng)基 , PANC-1 細(xì)胞 , IMDM 培養(yǎng)基 , HPAF-II 細(xì)胞 , MIA PaCa-2 細(xì)胞
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Preclinical evaluation of 89Zr/177Lu-labeled amatuximab for theranostic application in pancreatic ductal adenocarcinoma (2024-11-13)
期刊:INTERNATIONAL JOURNAL OF PHARMACEUTICS
DOI:10.1016/j.ijpharm.2024.124946
影響因子 :5.3
引用產(chǎn)品: MIA PaCa-2 細(xì)胞 , CFPAC-1 細(xì)胞 , PANC-1 細(xì)胞
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SERPINA1 promotes the invasion, metastasis, and proliferation of pancreatic ductal adenocarcinoma via the PI3K/Akt/NF-κB pathway (2024-10-18)
期刊:BIOCHEMICAL PHARMACOLOGY
影響因子 :5.3
引用產(chǎn)品: CFPAC-1 細(xì)胞 , BxPC-3 細(xì)胞
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Circ_0005397 inhibits ferroptosis of pancreatic cancer cells by up-regulating PCBP2 through KAT6A/H3K9Ac (2024-09-05)
期刊:FASEB JOURNAL
影響因子 :4.4
引用產(chǎn)品: CFPAC-1 細(xì)胞 , DMEM高糖 培養(yǎng)基
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HTF4 modulates the transcription of GID2 to promote the malignant biological behavior of pancreatic cancer (2024-08-13)
期刊:PANCREATOLOGY
影響因子 :2.8
引用產(chǎn)品: CFPAC-1 細(xì)胞 , IMDM 培養(yǎng)基 , Leibovitz's L-15 培養(yǎng)基
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MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression (2023-10-31)
期刊:EMBO JOURNAL
影響因子 :11.4
引用產(chǎn)品: 293T [HEK-293T] 細(xì)胞 , PANC-1 細(xì)胞 , CFPAC-1 細(xì)胞 , BxPC-3 細(xì)胞 , SW 1990 細(xì)胞
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METTL3 promotes pancreatic cancer proliferation and stemness by increasing stability of ID2 mRNA in a m6A-dependent manner (2023-05-19)
期刊:CANCER LETTERS
DOI:10.1016/j.canlet.2023.216222
影響因子 :9.7
引用產(chǎn)品: PC 細(xì)胞 , CFPAC-1 細(xì)胞 , HPDEC 細(xì)胞 , Panc02 細(xì)胞
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High glucose-upregulated PD-L1 expression through RAS signaling-driven downregulation of PTRH1 leads to suppression of T cell cytotoxic function in tumor environment (2023-07-11)
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-023-04302-4
影響因子 :7.4
引用產(chǎn)品: CFPAC-1 細(xì)胞 , Panc02 細(xì)胞
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Construction and comprehensive analysis of a novel prognostic signature associated with pyroptosis molecular subtypes in patients with pancreatic adenocarcinoma (2023-02-03)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2023.1111494
影響因子 :7.3
引用產(chǎn)品: CFPAC-1 細(xì)胞 , CFPAC-1細(xì)胞專用培養(yǎng)基
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Crosstalk of ferroptosis regulators and tumor immunity in pancreatic adenocarcinoma: novel perspective to mRNA vaccines and personalized immunotherapy (2023-06-27)
期刊:APOPTOSIS
DOI:10.1007/s10495-023-01868-8
影響因子 :7.2
引用產(chǎn)品: HPNE 細(xì)胞 , CFPAC-1 細(xì)胞 , SW 1990 細(xì)胞 , BxPC-3 細(xì)胞
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PSMD12 interacts with CDKN3 and facilitates pancreatic cancer progression (2023-04-10)
期刊:CANCER GENE THERAPY
DOI:10.1038/s41417-023-00609-y
影響因子 :6.4
引用產(chǎn)品: CFPAC-1 細(xì)胞 , IMDM 培養(yǎng)基 , Leibovitz's L-15 培養(yǎng)基
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5′-tRF-19-Q1Q89PJZ Suppresses the Proliferation and Metastasis of Pancreatic Cancer Cells via Regulating Hexokinase 1-Mediated Glycolysis (2023-10-12)
期刊:Biomolecules
影響因子 :5.5
引用產(chǎn)品: MIA PaCa-2 細(xì)胞 , PANC-1 細(xì)胞 , CFPAC-1 細(xì)胞 , Capan-2 細(xì)胞 , SW 1990 細(xì)胞 , BxPC-3 細(xì)胞 , AsPC-1 細(xì)胞 , 人胰腺上皮細(xì)胞
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RBM15 Promates the Proliferation, Migration and Invasion of Pancreatic Cancer Cell Lines (2023-02-08)
期刊:Cancers
影響因子 :5.2
引用產(chǎn)品: HPNE 細(xì)胞 , CFPAC-1 細(xì)胞 , BxPC-3 細(xì)胞
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COL12A1 Acts as a Novel Prognosis Biomarker and Activates Cancer-Associated Fibroblasts in Pancreatic Cancer through Bioinformatics and Experimental Validation (2023-02-26)
期刊:Cancers
影響因子 :5.2
引用產(chǎn)品: PANC-1 細(xì)胞 , BxPC-3 細(xì)胞 , CFPAC-1 細(xì)胞 , PATU8988 細(xì)胞 , AsPC-1 細(xì)胞 , MIA PaCa-2 細(xì)胞
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Identifying endoplasmic reticulum stress-related molecular subtypes and prognostic model for predicting the immune landscape and therapy response in pancreatic cancer (2023-10-09)
期刊:Aging-US
影響因子 :5.2
引用產(chǎn)品: CFPAC-1 細(xì)胞 , PANC-1 細(xì)胞
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GID2 Interacts With CDKN3 and Regulates Pancreatic Cancer Growth and Apoptosis (2023-02-23)
期刊:LABORATORY INVESTIGATION
DOI:10.1016/j.labinv.2023.100122
影響因子 :5.0
引用產(chǎn)品: CFPAC-1 細(xì)胞 , IMDM 培養(yǎng)基 , Leibovitz's L-15 培養(yǎng)基
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Identification of a chemoresistance-related prognostic gene signature by comprehensive analysis and experimental validation in pancreatic cancer (2023-05-12)
期刊:Frontiers in Oncology
影響因子 :4.7
引用產(chǎn)品: AsPC-1 細(xì)胞 , CFPAC-1 細(xì)胞 , PANC-1 細(xì)胞 , IMDM 培養(yǎng)基 , DMEM高糖 培養(yǎng)基 , 特級胎牛血清 , 青霉素-鏈霉素溶液 (雙抗),100×
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PANoptosis-related molecular subtype and prognostic model associated with the immune microenvironment and individualized therapy in pancreatic cancer (2023-07-14)
期刊:Frontiers in Oncology
影響因子 :4.7
引用產(chǎn)品: PANC-1 細(xì)胞 , CFPAC-1 細(xì)胞
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Identification and characterization of interferon-γ signaling-based personalized heterogeneity and therapeutic strategies in patients with pancreatic cancer (2023-10-24)
期刊:Frontiers in Oncology
影響因子 :4.7
引用產(chǎn)品: BxPC-3 細(xì)胞 , PANC-1 細(xì)胞 , CFPAC-1 細(xì)胞 , MIA PaCa-2 細(xì)胞
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Involvement of Ataxin-3 (ATXN3) in the malignant progression of pancreatic cancer via deubiquitinating HDAC6 (2023-07-07)
期刊:PANCREATOLOGY
影響因子 :3.6
引用產(chǎn)品: CFPAC-1 細(xì)胞
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A prospective prognostic signature for pancreatic adenocarcinoma based on ubiquitination-related mRNA-lncRNA with experimental validation in vitro and vivo (2023-08-04)
期刊:FUNCTIONAL & INTEGRATIVE GENOMICS
DOI:10.1007/s10142-023-01158-1
影響因子 :2.9
引用產(chǎn)品: CFPAC-1 細(xì)胞 , PANC-1 細(xì)胞 , IMEM
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ZKSCAN5 activates LAPTM5 expression by recruiting SETD7 to promote metastasis in pancreatic ductal adenocarcinoma. (2023-11-22)
期刊:HISTOLOGY AND HISTOPATHOLOGY
影響因子 :2.0
引用產(chǎn)品: 青霉素-鏈霉素溶液(雙抗),100× , AsPC-1 細(xì)胞 , Annexin V-FITC/PI熒光雙染細(xì)胞凋亡檢測試劑盒 , CFPAC-1 細(xì)胞 , Capan-1 細(xì)胞 , BxPC-3 細(xì)胞
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System analysis based on the pyroptosis-related genes identifies GSDMC as a novel therapy target for pancreatic adenocarcinoma (2022-10-05)
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-022-03632-z
影響因子 :8.4
引用產(chǎn)品: CFPAC-1 細(xì)胞 , PANC-1 細(xì)胞
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Synthesis and anti-tumor activity of nitrogen-containing derivatives of the natural product diphyllin (2022-08-26)
期刊:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1016/j.ejmech.2022.114708
影響因子 :7.1
引用產(chǎn)品: MIA PaCa-2 細(xì)胞 , CFPAC-1 細(xì)胞 , PaTu 8988t 細(xì)胞
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Upstream stimulatory factor 2 inhibits erastin-induced ferroptosis in pancreatic cancer through transcriptional regulation of pyruvate kinase M2 (2022-09-20)
期刊:BIOCHEMICAL PHARMACOLOGY
影響因子 :6.1
引用產(chǎn)品: PANC-1 細(xì)胞 , MIA PaCa-2 細(xì)胞 , BxPC-3 細(xì)胞 , AsPC-1 細(xì)胞 , CFPAC-1 細(xì)胞 , SW 1990 細(xì)胞 , CFPAC-1細(xì)胞專用培養(yǎng)基 , SW 1990細(xì)胞專用培養(yǎng)基 (L15)
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Anti?silencing function 1B promotes the progression of pancreatic cancer by activating c?Myc (2022-11-17)
期刊:INTERNATIONAL JOURNAL OF ONCOLOGY
影響因子 :5.9
引用產(chǎn)品: CFPAC-1 細(xì)胞 , HPAF-II 細(xì)胞 , 馬血清 , IMDM 培養(yǎng)基 , PANC-1 細(xì)胞 , MEM(含NEAA) 培養(yǎng)基 , MIA PaCa-2 細(xì)胞
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Construction and validation of a m6A RNA methylation and ferroptosis-related prognostic model for pancreatic cancer by integrated bioinformatics analysis. (2022-10-01)
期刊:Journal of Gastrointestinal Oncology
影響因子 :2.6
引用產(chǎn)品: CFPAC-1 細(xì)胞 , PANC-1 細(xì)胞 , BxPC-3 細(xì)胞
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miR-23a-5p inhibits cell proliferation and invasion in pancreatic ductal adenocarcinoma by suppressing ECM1 expression (2019-05-15)
期刊:American Journal of Translational Research
DOI:PMID:31217868
影響因子 :2.2
引用產(chǎn)品: AsPC-1 細(xì)胞 , CFPAC-1 細(xì)胞
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