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人椎間盤髓核細胞
Human Intervertebral Disc Nucleus Pulposu Cells
貨號：CP-H097
規(guī)格：5×10?Cells/T25培養(yǎng)瓶
價格： ￥詢價
培養(yǎng)基貨號： CM-H097
產(chǎn)品概述
人椎間盤髓核細胞
Cat NO.: CP-H097
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產(chǎn)品名稱：人椎間盤髓核細胞
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英文簡稱：NPCs
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組織來源：椎間盤組織
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產(chǎn)品規(guī)格：5×10?Cells/T25培養(yǎng)瓶
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細胞簡介：
人椎間盤髓核細胞分離椎間盤組織；椎間盤是位于脊柱兩椎體之間，分為中央部的髓核，富于彈性的膠狀物質(zhì)；周圍部的纖維環(huán)，由多層纖維軟骨環(huán)按同心圓排列。上下有軟骨板，是透明軟骨復(fù)蓋于椎體上，下面骺環(huán)中間的骨面。上下的軟骨板與纖維環(huán)一起將髓核密封起來。纖維環(huán)由膠原纖維束的纖維軟骨構(gòu)成，位于髓核的四周。纖維環(huán)的纖維束相互斜行交叉重疊，使纖維環(huán)成為堅實的組織，能承受較大的彎曲和扭轉(zhuǎn)負荷。髓核，是乳白色半透明膠狀體，富于彈性，為椎間盤結(jié)構(gòu)的一部分，位于兩軟骨板與纖維環(huán)之間。由縱橫交錯的纖維網(wǎng)狀結(jié)構(gòu)即軟骨細胞和蛋白多糖黏液樣基質(zhì)構(gòu)成的彈性膠凍物質(zhì)。嬰幼兒時期的髓核含水量為80%-90%，即使到了老年，其含水量也在70%上下。髓核在出生時體積大而松散，位于椎間盤的中央，至成年時位置移至椎間盤的中后部；在成年以前構(gòu)成髓核的主要物質(zhì)是大量蛋白多糖復(fù)合體、膠原纖維和纖維軟骨，隨著年齡的增長，髓核中的蛋白多糖解聚增多，水分逐漸減少，膠原增粗并逐漸被纖維軟骨所替代。
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方法簡介：
普諾賽實驗室分離的人椎間盤髓核細胞采用膠原酶-中性蛋白酶混合消化法并結(jié)合軟骨細胞專用培養(yǎng)基培養(yǎng)篩選制備而來，細胞總量約為5×10?cells/瓶。
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質(zhì)量檢測：
普諾賽實驗室分離的人椎間盤髓核細胞經(jīng)Ⅱ型膠原蛋白免疫熒光鑒定，純度可達90%以上，且不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌等。
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培養(yǎng)信息：
包被條件 培養(yǎng)基 含F(xiàn)BS、生長添加劑、Penicillin、Streptomycin等 產(chǎn)品貨號 CM-H097 換液頻率 每3-4天換液一次 生長特性 貼壁 細胞形態(tài) 梭形、多角形 傳代特性 可傳5代左右；3代以內(nèi)狀態(tài)最佳 消化液 0.25%胰蛋白酶 人椎間盤髓核細胞體外培養(yǎng)周期有限；建議使用普諾賽配套的專用生長培養(yǎng)基及正確的操作方法來培養(yǎng)，以此保證該細胞的最佳培養(yǎng)狀態(tài)。
參考文獻
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PDE4B promotes JNK/NLRP3 activation in the nucleus pulposus and mediates intervertebral disc degeneration (2025-02-17)
期刊：Scientific Reports
DOI：10.1038/s41598-025-88053-w
影響因子 ：3.8
引用產(chǎn)品： 人椎間盤髓核細胞 , 人椎間盤髓核細胞完全培養(yǎng)基
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MANF overexpression ameliorates oxidative stress-induced apoptosis of human nucleus pulposus cells by facilitating mitophagy through promoting MFN2 expression (2025-01-02)
期刊：Scientific Reports
DOI：10.1038/s41598-024-84167-9
影響因子 ：3.8
引用產(chǎn)品： 人椎間盤髓核細胞
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“Dictionary of immune responses” reveals the critical role of monocytes and the core target IRF7 in intervertebral disc degeneration (2024-10-17)
期刊：Frontiers in Immunology
DOI：10.3389/fimmu.2024.1465126
影響因子 ：5.7
引用產(chǎn)品： 人椎間盤髓核細胞
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Platelet-rich plasma-derived extracellular vesicles inhibit NF-κB/NLRP3 pathway-mediated pyroptosis in intervertebral disc degeneration via the MALAT1/microRNA-217/SIRT1 axis (2024-02-17)
期刊：CELLULAR SIGNALLING
DOI：10.1016/j.cellsig.2024.111106
影響因子 ：4.8
引用產(chǎn)品： 人椎間盤髓核細胞 , 人椎間盤髓核細胞完全培養(yǎng)基
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Apoptosis Signal-Regulated Kinase-1 Promotes Nucleus Pulposus Cell Senescence and Apoptosis to Regulate Intervertebral Disc Degeneration (2024-06-13)
期刊：AMERICAN JOURNAL OF PATHOLOGY
DOI：10.1016/j.ajpath.2024.05.004
影響因子 ：4.7
引用產(chǎn)品： 人椎間盤髓核細胞
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Identification and experimental verification of immune-related hub genes in intervertebral disc degeneration (2024-07-13)
期刊：Heliyon
DOI：10.1016/j.heliyon.2024.e34530
影響因子 ：3.4
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Ginsenoside Rg3 exhibits anti-catabolic and anti-apoptotic effects in IL-1β treated human disc nucleus pulposus cells and in a rat model of disc degeneration by inactivating the MAPK pathway (2024-01-31)
期刊：CELLULAR AND MOLECULAR BIOLOGY
影響因子 ：1.5
引用產(chǎn)品： 人椎間盤髓核細胞
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Trigonochinene E promotes lysosomal biogenesis and enhances autophagy via TFEB/TFE3 in human degenerative NP cells against oxidative stress (2023-02-18)
期刊：PHYTOMEDICINE
DOI：10.1016/j.phymed.2023.154720
影響因子 ：7.9
引用產(chǎn)品： 人椎間盤髓核細胞
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Pharmacological network analysis of the functions and mechanism of kaempferol from Du Zhong in intervertebral disc degeneration (IDD) (2023-03-03)
期刊：Journal of Orthopaedic Translation
影響因子 ：6.6
引用產(chǎn)品： 人椎間盤髓核細胞
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hASCs-derived exosomal miR-155-5p targeting TGFβR2 promotes autophagy and reduces pyroptosis to alleviate intervertebral disc degeneration (2023-03-14)
期刊：Journal of Orthopaedic Translation
影響因子 ：6.6
引用產(chǎn)品： 人椎間盤髓核細胞 , 人脂肪間充質(zhì)干細胞
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BSHXF-medicated serum combined with ADSCs regulates the TGF-β1/Smad pathway to repair oxidatively damaged NPCs and its component analysis (2023-06-03)
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影響因子 ：5.4
引用產(chǎn)品： 人椎間盤髓核細胞
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microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3 (2023-11-27)
期刊：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影響因子 ：5.3
引用產(chǎn)品： 人椎間盤髓核細胞 , 青霉素-鏈霉素溶液 (雙抗)，100×
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The long non-coding RNA maternally expressed 3-micorRNA-15a-5p axis is modulated by melatonin and prevents nucleus pulposus cells inflammation and apoptosis (2023-09-01)
期刊：BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
影響因子 ：3.1
引用產(chǎn)品： 人椎間盤髓核細胞
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Retinoic acid inhibits the pyroptosis of degenerated nucleus pulposus cells by activating Sirt1-SOD2 signaling (2023-04-02)
期刊：CONNECTIVE TISSUE RESEARCH
DOI：10.1080/03008207.2023.2192286
影響因子 ：2.9
引用產(chǎn)品： 人椎間盤髓核細胞
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Overloaded axial stress activates the Wnt/β-Catenin pathway in nucleus pulposus cells of adult degenerative scoliosis combined with intervertebral disc degeneration (2023-04-08)
期刊：MOLECULAR BIOLOGY REPORTS
DOI：10.1007/s11033-023-08390-9
影響因子 ：2.8
引用產(chǎn)品： 人椎間盤髓核細胞
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LINC01116 Regulates the Proliferation and Apoptosis of Nucleus Pulposus Cells through miR-9-5p-mediated ZIC5 and the Wnt Pathway and Affects the Progression of Intervertebral Disc Degeneration (2023-10-01)
期刊：Current Stem Cell Research & Therapy
DOI：10.2174/1574888X17666220804105305
影響因子 ：2.7
引用產(chǎn)品： 人椎間盤髓核細胞
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Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway. (2023-03-01)
期刊：JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS
DOI：PMID:36856108
影響因子 ：1.9
引用產(chǎn)品： 人椎間盤髓核細胞
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Mesenchymal stem cell-derived exosomes are beneficial to suppressing inflammation and promoting autophagy in intervertebral disc degeneration (2023-03-22)
期刊：FOLIA MORPHOLOGICA
影響因子 ：1.2
引用產(chǎn)品： 人椎間盤髓核細胞
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Exogenous Klotho ameliorates extracellular matrix degradation and angiogenesis in intervertebral disc degeneration via inhibition of the Rac1/PAK1/MMP-2 signaling axis (2022-08-08)
期刊：MECHANISMS OF AGEING AND DEVELOPMENT
影響因子 ：5.5
引用產(chǎn)品： 人椎間盤髓核細胞
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The protective effects of dezocine on interleukin-1β-induced inflammation, oxidative stress and apoptosis of human nucleus pulposus cells and the possible mechanisms (2022-01-03)
期刊：Bioengineered
DOI：10.1080/21655979.2021.2017700
影響因子 ：4.9
引用產(chǎn)品： 人椎間盤髓核細胞
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Kukoamine A attenuates lipopolysaccharide-induced apoptosis, extracellular matrix degradation, and inflammation in nucleus pulposus cells by activating the P13K/Akt pathway (2022-03-25)
期刊：Bioengineered
DOI：10.1080/21655979.2022.2051855
影響因子 ：4.9
引用產(chǎn)品： 人椎間盤髓核細胞
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MicroRNA-137 inhibits the inflammatory response and extracellular matrix degradation in lipopolysaccharide-stimulated human nucleus pulposus cells by targeting activin a receptor type I (2022-03-02)
期刊：Bioengineered
DOI：10.1080/21655979.2022.2042987
影響因子 ：4.9
引用產(chǎn)品： 人椎間盤髓核細胞
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miR-4478 Accelerates Nucleus Pulposus Cells Apoptosis Induced by Oxidative Stress by Targeting MTH1 (2022-09-20)
期刊：SPINE
DOI：10.1097/BRS.0000000000004486
影響因子 ：3.2
引用產(chǎn)品： 人椎間盤髓核細胞
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Arctigenin inhibits apoptosis, extracellular matrix degradation, and inflammation in human nucleus pulposus cells by up-regulating miR-483-3p (2022-06-11)
期刊：JOURNAL OF CLINICAL LABORATORY ANALYSIS
影響因子 ：3.1
引用產(chǎn)品： 人椎間盤髓核細胞 , 人椎間盤髓核細胞完全培養(yǎng)基
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Lumican silencing alleviates tumor necrosis factor-α-induced nucleus pulposus cell inflammation and senescence by inhibiting apoptosis signal regulating kinase 1/p38 signaling pathway via inactivating Fas ligand expression (2021-09-13)
期刊：Bioengineered
DOI：10.1080/21655979.2021.1973781
影響因子 ：4.9
引用產(chǎn)品： 人椎間盤髓核細胞
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miR?489?3p overexpression inhibits lipopolysaccharide?induced nucleus pulposus cell apoptosis, inflammation and extracellular matrix degradation via targeting Toll?like receptor 4 (2021-09-20)
期刊：Experimental and Therapeutic Medicine
影響因子 ：2.7
引用產(chǎn)品： 人椎間盤髓核細胞
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MiR-330-5p inhibits intervertebral disk degeneration via targeting CILP (2021-07-07)
期刊：Journal of Orthopaedic Surgery and Research
DOI：10.1186/s13018-021-02582-4
影響因子 ：2.6
引用產(chǎn)品： 人椎間盤髓核細胞
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CircRNA RERE Promotes the Oxidative Stress-Induced Apoptosis and Autophagy of Nucleus Pulposus Cells through the miR-299-5p/Galectin-3 Axis (2021-12-15)
期刊：Journal of Healthcare Engineering
影響因子 ：0.0
引用產(chǎn)品： 人椎間盤髓核細胞
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Micro Fragmented Adipose Tissue Promotes the Matrix Synthesis Function of Nucleus Pulposus Cells and Regenerates Degenerated Intervertebral Disc in a Pig Model: (2020-02-07)
期刊：Cell Transplantation
影響因子 ：3.3
引用產(chǎn)品： 人椎間盤髓核細胞
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Role of lncRNA PART1 in intervertebral disc degeneration and associated underlying mechanism (2020-12-10)
期刊：Experimental and Therapeutic Medicine
影響因子 ：2.7
引用產(chǎn)品： 人椎間盤髓核細胞
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Dimethyl fumarate protects nucleus pulposus cells from inflammation and oxidative stress and delays the intervertebral disc degeneration (2020-10-27)
期刊：Experimental and Therapeutic Medicine
影響因子 ：2.7
引用產(chǎn)品： 人椎間盤髓核細胞
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