THLE-2（人肝永生化細(xì)胞）(STR鑒定正確)

文獻(xiàn)(38) 說明書

一鍵復(fù)制產(chǎn)品信息

英文名稱：THLE-2

貨號： CL-0833

規(guī)格：

1×10^6Cells/T25 1×10^6Cells/Vial×2Vials

套餐1

THLE-2（人肝永生化細(xì)胞）

￥2500

THLE-2細(xì)胞專用培養(yǎng)基

￥ 2480.00 ￥2680

細(xì)胞培養(yǎng)最優(yōu)解！

套餐組合價

￥ 4980 ￥5180

產(chǎn)品信息

產(chǎn)品名稱	THLE-2（人肝永生化細(xì)胞）
別稱	THLE2;Transformed Human Liver Epithelial-2
種屬	人
生長特性	貼壁細(xì)胞
細(xì)胞形態(tài)	上皮細(xì)胞樣
凍存條件	凍存液：55% DM/F12+40% FBS+5% DMSO 溫度：液氮
培養(yǎng)方案A（默認(rèn)）	生長培養(yǎng)基：THLE-2細(xì)胞專用培養(yǎng)基培養(yǎng)條件：氣相：空氣，95%；CO?，5%, 溫度：37℃
推薦傳代比例	1:3-1:6
推薦換液頻率	2-3次/周

參考資料(來源文獻(xiàn))

背景描述	THLE-2 is an epithelial cell that was isolated from the left lobe of a donor.THLE-2 and THLE-3 cells express phenotypic characteristics of normal adult liver epithelial cells. They are nontumorigenic when injected into athymic nude mice, have near-diploid karyotypes, and do not express alpha-fetoprotein. [RF84750] THLE-2 and THLE-3 cells metabolize benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 to their ultimate carcinogenic metabolites that adduct DNA, which indicates functional cytochrome P450 pathways.
年齡（性別）	Adult;Male
組織來源	Liver; Left lobe
細(xì)胞類型	轉(zhuǎn)化細(xì)胞系
生物安全等級	BSL-2
致瘤性	No; No, nude mice.
保藏機(jī)構(gòu)	ATCC; CRL-2706

位點信息

鑒定圖

Deubiquitinase USP28 promotes the malignant progression and radio-resistance of hepatocellular carcinoma by stabilizing WDHD1(2025)

期刊：NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY

DOI：10.1007/s00210-025-03793-w

影響因子：3.1

引用產(chǎn)品： THLE-2細(xì)胞專用培養(yǎng)基, HuH-7 細(xì)胞, THLE-2 細(xì)胞, DMEM高糖培養(yǎng)基
Inotodiol induces hepatocellular carcinoma apoptosis by activation of MAPK/ERK pathway(2025)

期刊：PLoS One

DOI：10.1371/journal.pone.0318450

影響因子：2.9

引用產(chǎn)品： AML12 細(xì)胞, THLE-2 細(xì)胞
AURKB as a Therapeutic Target and Key Driver of Liver Cancer Growth and Metastasis(2025)

期刊：APMIS

DOI：10.1111/apm.70021

影響因子：2.2

引用產(chǎn)品： THLE-2 細(xì)胞
E2F1-driven EXOSC10 transcription promotes hepatocellular carcinoma growth and stemness: a potential therapeutic target(2025)

期刊：HEREDITAS

DOI：10.1186/s41065-025-00430-7

影響因子：2.1

引用產(chǎn)品：青霉素-鏈霉素溶液（雙抗），100×, THLE-2 細(xì)胞, HuH-7 細(xì)胞, Hep 3B2.1-7 細(xì)胞
Inhibition of hepatocellular carcinoma progression by artesunate via modulation of the TLR4/MyD88/NF-κB signaling pathway(2025)

期刊：Journal of Gastrointestinal Oncology

DOI：10.21037/jgo-2025-95

影響因子：2

引用產(chǎn)品： THLE-2 細(xì)胞, Hep G2 細(xì)胞, Hep-G2/C3A 細(xì)胞, HuH-7 細(xì)胞, SNU-182 細(xì)胞, HLE 細(xì)胞
MiR-518b Promotes the Tumorigenesis of Hepatocellular Carcinoma by Targeting EGR1 to Regulate PI3K/AKT/mTOR Signaling Pathway(2025)

期刊：CELL BIOCHEMISTRY AND BIOPHYSICS

DOI：10.1007/s12013-025-01752-z

影響因子：1.8

引用產(chǎn)品： HuH-7 細(xì)胞, THLE-2 細(xì)胞
MICAL1 Mediates TGF-β1-Induced Epithelial-to-Mesenchymal Transition and Metastasis of Hepatocellular Carcinoma by Activating Smad2/3(2025)

期刊：CELL BIOCHEMISTRY AND BIOPHYSICS

DOI：10.1007/s12013-025-01668-8

影響因子：1.8

引用產(chǎn)品： TGFB1/TGFβ1/TGF-β1, Human, Recombinant, THLE-2 細(xì)胞
Comprehensive assessment of cleavage and polyadenylation specificity factors in hepatocellular carcinoma: Expression, prognostic significance and immune infiltration analysis(2025)

期刊：Molecular and Clinical Oncology

DOI：10.3892/mco.2025.2855

影響因子：1.4

引用產(chǎn)品： THLE-2 細(xì)胞
Accumulation of TOX high mobility group box family member 3 promotes the oncogenesis and development of hepatocellular carcinoma through the MAPK signaling pathway(2024)

期刊：MedComm

DOI：10.1002/mco2.510

影響因子：9.9

引用產(chǎn)品： THLE-2 細(xì)胞
O-GlcNAcylated RALY Contributes to Hepatocellular Carcinoma Cells Proliferation by Regulating USP22 mRNA Nuclear Export(2024)

期刊：International Journal of Biological Sciences

DOI：10.7150/ijbs.97397

影響因子：8.2

引用產(chǎn)品： HuH-7 細(xì)胞, THLE-2 細(xì)胞, Hep 3B2.1-7 細(xì)胞

Q1:THLE-2細(xì)胞是如何誘導(dǎo)永生化的？

查閱資料顯示：THLE-2人肝永生化貼壁細(xì)胞系是正常肝細(xì)胞用SV40大T抗原轉(zhuǎn)染得到。
Q2:THLE-2 細(xì)胞在我的實驗中，細(xì)胞用藥后要經(jīng)過饑餓處理，這個過程中細(xì)胞不能用完全培養(yǎng)基，應(yīng)該用什么培養(yǎng)基培養(yǎng)？

可以使用DMEM/F12培養(yǎng)基(貨號：PM150312），營養(yǎng)成分較為豐富適合絕大部分細(xì)胞系。