OPG/TNFRSF11B (C-6His), Human, Recombinant
英文名稱：OPG/TNFRSF11B (C-6His), Human, Recombinant
貨號： PCK213
價格： ￥320 ￥980 ￥6080 ￥8680
規(guī)格：
10μg
50μg
500μg
1mg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Tumor Necrosis Factor Receptor Superfamily Member 11B;Osteoclastogenesis Inhibitory Factor;Osteoprotegerin;TNFRSF11B;OCIF;OPG |
| 物種 | 人 |
| 表達宿主 | Human Cells |
| 序列信息 | Glu22-Leu401 |
| 檢索號 | O00300 |
| 分子量 | 44.65 kDa |
| 表觀分子量 | 57 kDa |
| 生物活性 | Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 10.6 ng/mL. |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4. |
| 復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | TNFRSF11B is a secreted Protein, containing 2 death domains and 4 TNFR-Cys repeats. TNFRSF11B is a decoy Receptor for the Receptor activator of nuclear factor kappa B Ligand (RANKL). By binding RANKL, TNFRSF11B inhibits nuclear kappa B (NF-κB) which is a central and rapid acting transcription factor for immune-related genes, and a key regulator of inflammation, innate immunity, and cell survival and differentiation. TNFRSF11B levels are influenced by voltage-dependent calcium channelsCav1.2. TNFRSF11B can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors (osteoclasts are related to monocytes/macrophages and are Derived from granulocyte/macrophage-forming colony units (CFU-GM)) into osteoclasts and also regulates the resorption of osteoclasts in vitroand in vivo. TNFRSF11B binding to RANKL on osteoblast/stromal cells, blocks the RANKL-RANK Ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast. |
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Q1:官網(wǎng)的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實驗時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進一步稀釋才行呢？
如果是直接做實驗，可以直接添加培養(yǎng)基進行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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