Interferon γ/IFN-γ/IFNG, Human, Recombinant
英文名稱：Interferon γ/IFN-γ/IFNG, Human, Recombinant
貨號： PCK062
價格： ￥2280 ￥1280 ￥780 ￥300 ￥3480
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 500μg / 100μg / 50μg / 10μg / 1mg |
| 別名 | Type II interferon;T-cell interferon;MAF |
| 物種 | 人 |
| 表達宿主 | E.coli |
| 序列信息 | MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFQGRRASQ with polyhistidine tag at the C-terminus. |
| 檢索號 | P01579.1 |
| 分子量 | 17.7 kDa |
| 表觀分子量 | 17 kDa |
| 標簽 | His-tag at the C-terminus |
| 生物活性 | His-tag at the C-terminus |
| 純度 | > 95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 內(nèi)毒素 | < 0.01 EU per 1 μg of the protein by the LAL method. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0. |
| 復融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 背景介紹 | Interferon-γ is a effective multifunctional cytokine which is released mainly by activated NK cells and T cells. IFN-γ is primarily characterized based on its anti-viral activities, and then been proved to have several functions such as anti-proliferative, immune-regulatory, and pro-inflammatory activities. IFN-γ can upregulate expression of MHC class I and II antigen by antigen-presenting cells. |
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期刊：RENAL FAILURE
DOI：10.1080/0886022X.2024.2438859
影響因子：3.0
引用產(chǎn)品： Interferon γ/IFN-γ/IFNG, Human, Recombinant, 人腎足突細胞, RPMI-1640 培養(yǎng)基
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期刊：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影響因子：4.3
引用產(chǎn)品： RPMI-1640 培養(yǎng)基, 293T [HEK-293T] 細胞, MEM（含NEAA） 培養(yǎng)基, 青霉素-鏈霉素溶液（雙抗），100×, HK-2 細胞, 人腎足突細胞, DMEM高糖 培養(yǎng)基, Interferon γ/IFN-γ/IFNG, Human, Recombinant, 特級胎牛血清
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Q1:因子到貨了，里面有兩管溶液，一個是水，一個是海藻糖溶液，用哪一個稀釋呢？
1、將裝有細胞因子的管子放入小離心機內(nèi)短暫離心20~30s（離心機最大轉速即可），使附著在管壁的微量蛋白聚集在管底； 2、先用無菌水溶解因子，很少量水即可溶解因子，注意計算溶解后濃度不低于100 μg/mL； 3、用贈送的含海藻糖的PBS溶液進一步稀釋到您實驗合適的濃度即可，然后分裝后放入-20℃以下保藏； 4、因子復融重懸過程應輕柔，不可以震蕩渦旋。
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