IL-9, Mouse, Recombinant
英文名稱:IL-9, Mouse, Recombinant
貨號(hào): PCK246
價(jià)格: ¥1180 ¥3480 ¥11880 ¥18880
規(guī)格:
10μg
50μg
500μg
1mg
產(chǎn)品信息
產(chǎn)品形態(tài) | 粉末 |
產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
別名 | Interleukin-9;IL-9;Cytokine P40;T-Cell Growth Factor P40;IL9 |
物種 | 小鼠 |
表達(dá)宿主 | Human Cells |
序列信息 | Gln19-Pro144 |
檢索號(hào) | P15247 |
分子量 | 15.2 kDa |
表觀分子量 | 28-42 kDa |
標(biāo)簽 | C-6His |
純度 | > 95% as determined by reducing SDS-PAGE. |
內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
儲(chǔ)存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
運(yùn)輸條件 | Ambient temperature or ice pack. |
有效期 | 12個(gè)月 |
制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介紹 | Interleukin-9 (IL-9) is a secreted Protein that belongs to the IL-7/IL-9 family.Mature mouse IL-9 shares 57% and 74% amino acid sequence identity with human and rat IL-9, respectively. IL-9 supports IL-2 independent and IL-4 independent growth of helper T-cells. IL-9 stimulates cell proliferation and prevents apoptosis. It functions through the IL-9 Receptor (IL-9R), which activates different signal transducer and activator (STAT) Proteins and thus connects this Cytokine to various biological processes. IL-9 has been identified as a candidate gene for asthma. IL-9 is a determining factor in the pathogenesis of bronchial hyperresponsiveness. |
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么?
我們公司的因子都是重組的,是不含動(dòng)物源成分的。
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Q2:細(xì)胞因子用無(wú)菌水配成了100ug/mL母液,用于實(shí)驗(yàn)時(shí),需要的終濃度為10ng/mL,可以用培養(yǎng)基直接稀釋?還是需要通過PBS(含5%海藻糖)進(jìn)一步稀釋才行呢?
如果是直接做實(shí)驗(yàn),可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長(zhǎng)時(shí)間保存的話,需要使用含載體蛋白(如0.1%BSA,10% FBS,5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無(wú)菌水溶解后需要再次離心嗎?
加了無(wú)菌水后,如果沒有掛壁現(xiàn)象是不用離心的,如果您放置了一段時(shí)間,再次使用時(shí)是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎?
不用提前放置室溫.

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