hCMEC/D3（人腦微血管內(nèi)皮細(xì)胞）
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英文名稱：hCMEC/D3
貨號： CL-0843
價格： ￥2000
套餐組合價
產(chǎn)品信息
| 產(chǎn)品名稱 | hCMEC/D3（人腦微血管內(nèi)皮細(xì)胞） |
| 別稱 | HCMEC/D3;CMEC/D3;human Cortical Microvessels Endothelial Cells/D3 |
| 種屬 | 人 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 紡錘形細(xì)胞 |
| 凍存條件 |
凍存液：55% 基礎(chǔ)培養(yǎng)基+40% FBS+5% DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
生長培養(yǎng)基：hCMEC/D3細(xì)胞專用培養(yǎng)基[GCM-0843] 培養(yǎng)條件：氣相：空氣，95%；CO?，5%, 溫度：37℃ |
| 推薦傳代比例 | 1:3-1:6 |
| 推薦換液頻率 | 2-3次/周 |
參考資料(來源文獻(xiàn))
| 背景描述 | The hCMEC/D3 cell line was derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. The primary isolate was enriched in cerebral endothelial cells (CECs). In the first passage, cells were sequentially immortalized by lentiviral vector transduction with the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen, following which CEC were selectively isolated by limited dilution cloning, and clones were extensively characterized for brain endothelial phenotype. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). |
| 年齡（性別） | Adult;Female |
| 組織來源 | 腦微血管 |
| 細(xì)胞類型 | 轉(zhuǎn)化細(xì)胞系 |
| 生物安全等級 | BSL-2 |
位點信息
鑒定圖
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Drop-shaped microgrooves guide unidirectional cell migration for enhanced endothelialization(2025)
期刊：Nature Communications
DOI：10.1038/s41467-025-57146-5
影響因子：14.7
引用產(chǎn)品： hCMEC/D3 細(xì)胞
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期刊：FREE RADICAL BIOLOGY AND MEDICINE
DOI：10.1016/j.freeradbiomed.2024.10.288
影響因子：7.1
引用產(chǎn)品： SH-SY5Y [SHSY-5Y] 細(xì)胞, hCMEC/D3 細(xì)胞
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期刊：ANALYTICA CHIMICA ACTA
影響因子：5.7
引用產(chǎn)品： LN229 [LN-229; LNT-229] 細(xì)胞, NIH/3T3 細(xì)胞, Hela 細(xì)胞, HL-7702 [L-02; LO2] 細(xì)胞, SCC7 細(xì)胞, B16-F10 細(xì)胞, U251 細(xì)胞, Hep G2 細(xì)胞, hCMEC/D3 細(xì)胞, LLC [LL/2; LLC1] 細(xì)胞, PC-9 細(xì)胞, 4T1 細(xì)胞
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期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2024.150431
影響因子：2.5
引用產(chǎn)品： hCMEC/D3 細(xì)胞
識別碼示意圖
