Flt-3L/Flt3LG (C-6His), Mouse, Recombinant
英文名稱:Flt-3L/Flt3LG (C-6His), Mouse, Recombinant
貨號: PCK096
價格: ¥880 ¥2780 ¥11880 ¥17280
產(chǎn)品信息
產(chǎn)品形態(tài) | 粉末 |
產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
別名 | Fms-related tyrosine kinase 3 Ligand (Flt3L);SL Cytokine;Flt3 Ligand |
物種 | 小鼠 |
表達(dá)宿主 | Human Cells |
序列信息 | Gly27-Arg188 |
檢索號 | P49772 |
分子量 | 19.4 kDa |
表觀分子量 | 22-30 kDa |
標(biāo)簽 | C-6His |
純度 | > 95% as determined by reducing SDS-PAGE. |
內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
儲存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
運輸條件 | Ambient temperature or ice pack. |
有效期 | 12個月 |
制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介紹 | Fms-related tyrosine kinase 3 Ligand (Flt3L) is a single-pass type I membrane Protein and consists of 232 amino acids. Flt3L is a hematopoietic four helical bundle Cytokine, structurally homologous to stem cell factor and colony stimulating factor. Flt3L synergizes well with a number of other colony stimulating factors and Interleukins. Flt3L stimulates the proliferation and differentiation of various blood cell progentiors by activating FLT3. |
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Q1:官網(wǎng)的因子都是不含動物成分的么?
我們公司的因子都是重組的,是不含動物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液,用于實驗時,需要的終濃度為10ng/mL,可以用培養(yǎng)基直接稀釋?還是需要通過PBS(含5%海藻糖)進(jìn)一步稀釋才行呢?
如果是直接做實驗,可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長時間保存的話,需要使用含載體蛋白(如0.1%BSA,10% FBS,5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎?
加了無菌水后,如果沒有掛壁現(xiàn)象是不用離心的,如果您放置了一段時間,再次使用時是可以使用離心機離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎?
不用提前放置室溫.
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Q5:收到細(xì)胞因子產(chǎn)品用什么溶劑復(fù)融?
1、細(xì)胞因子產(chǎn)品大部分為凍干形式發(fā)貨,少部分液體形式發(fā)貨; 2、凍干粉狀態(tài)的因子產(chǎn)品需指定溶劑復(fù)溶,請參考產(chǎn)品說明書【復(fù)融】方法,按照要求使用對應(yīng)溶劑溶解,大部分因子為無菌水復(fù)融,少部分需要無菌水以外的特殊溶劑溶解的產(chǎn)品則隨貨會贈送相應(yīng)的復(fù)融試劑;凍干粉溶解后如您需要進(jìn)一步稀釋分裝,需使用含載體蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存。 3、液體形式因子產(chǎn)品不需要復(fù)溶,具體濃度詳見產(chǎn)品標(biāo)簽,按照實驗需要用含載體蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存即可;

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