FGF-8B, Human, Recombinant
英文名稱：FGF-8B, Human, Recombinant
貨號： PCK225
價格： ￥780 ￥2280 ￥11880 ￥17280
規(guī)格：
10μg
50μg
500μg
1mg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Fibroblast Growth Factor 8;Androgen-induced Growth Factor;Heparin-binding Growth Factor 8;AIGF;HBGF-8;FGF-8B |
| 物種 | 人 |
| 表達(dá)宿主 | E.coli |
| 序列信息 | Gln23-Arg215 |
| 檢索號 | P55075-3/P37237-2 |
| 分子量 | 22.5 kDa |
| 表觀分子量 | 23 kDa |
| 生物活性 | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 9.32 ng/mL. |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運(yùn)輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 300 mM NaCl, 2% Sucrose, 0.02% Tween 80, pH7.4. |
| 復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | Fibroblast Growth Factor 8 (FGF-8) is a member of the fibroblast Growth Factor family. It is discovered as a Growth Factor essential for the androgen-dependent growth of mouse mammary carcinoma cells. Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. It is required for normal brain, eye, ear, limb development during embryogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. |
-
Q1:官網(wǎng)的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
-
Q2:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
-
Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機(jī)離心的
-
Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
注：百度瀏覽器無法支持掃一掃功能，請輸入識別碼，獲取相應(yīng)產(chǎn)品資料
識別碼示意圖
×
![]()
