IL-1R-2/CD121b (C-6His), Mouse, Recombinant
英文名稱：IL-1R-2/CD121b (C-6His), Mouse, Recombinant
貨號： PCK239
價格： ￥780 ￥1880 ￥11880 ￥17280
規(guī)格：
10μg
50μg
500μg
1mg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Interleukin-1 Receptor type 2;IL-1R-2;IL-1RT-2;IL-1RT2;CD121 antigen-like family member B;CD121b;IL-1 type II Receptor;Interleukin-1 Receptor beta;IL-1R-beta;Interleukin-1 Receptor type II;CD121b |
| 物種 | 小鼠 |
| 表達宿主 | Human Cells |
| 序列信息 | Phe14-Glu355 |
| 檢索號 | P27931 |
| 分子量 | 39.0 kDa |
| 表觀分子量 | 45-60 kDa |
| 標簽 | C-6His |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 復融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | Mouse Interleukin 1 Receptor, type II (IL1R2) is a Cytokine Receptor that belongs to the Interleukin-1 Receptor family. This Protein binds Interleukin alpha (IL1A), Interleukin beta (IL1B), and Interleukin 1 Receptor, type I (IL1R1/IL1RA), and acts as a decoy Receptor that inhibits the activity of its Ligands. IL-1R2 structurally consisting of a Ligand binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain. It is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Mouse IL1RII shares 59% amino acid sequence homology with human IL1 RII in their extracellular domains. The pleiotropic Cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses, and binds to specific plasma membrane Receptors on cells. Two distinct types of IL1 Receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2 has no signaling property and acts as a decoy for IL-1. |
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Q1:官網(wǎng)的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q2:細胞因子用無菌水配成了100ug/mL母液，用于實驗時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進一步稀釋才行呢？
如果是直接做實驗，可以直接添加培養(yǎng)基進行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機離心的
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Q4:細胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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