APRIL/TNFSF13/TRDL-1α (N-Flag-His), Human, Recombinant
英文名稱：APRIL/TNFSF13/TRDL-1α (N-Flag-His), Human, Recombinant
貨號： PCK107
價格： ￥780 ￥1680 ￥14680 ￥19680
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Tumor necrosis factor Ligand superfamily member 13;A proliferation-inducing Ligand;APRIL;TNF- and APOL-related leukocyte expressed Ligand 2;TALL-2;TNF-related death Ligand 1;TRDL-1;CD256;TNFSF13 |
| 物種 | 人 |
| 表達(dá)宿主 | Human Cells |
| 序列信息 | Lys112-Leu250 |
| 檢索號 | O75888 |
| 分子量 | 50 kDa |
| 表觀分子量 | 60 kDa |
| 生物活性 | Immobilized Human APRIL -Flag-His at 1 μg/mL (100 μL/well) can bind Cynomolgus BCMA-Fc. The ED50 of Cynomolgus BCMA-Fc is 4.23 ng/mL. |
| 純度 | > 90% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 1 M NaCl, pH 8.3. |
| 復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | APRIL (a proliferation-inducingLigand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF Ligand superfamily. It issynthesized as a32 kDa pro Protein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule.Secreted human APRIL, which consists almost entirely ofa single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL. BothAPRIL and its close relative BAFF bind and signalthrough the TNF superfamily Receptors TACI and BCMA, while BAFF additionally functions through BAFF R.APRIL binds to heparan sulfateproteoglycans (HSPGs) independently of its binding to TACI and BCMA. |
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Q1:官網(wǎng)的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實驗時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實驗，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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Q5:收到細(xì)胞因子產(chǎn)品用什么溶劑復(fù)融？
1、細(xì)胞因子產(chǎn)品大部分為凍干形式發(fā)貨，少部分液體形式發(fā)貨； 2、凍干粉狀態(tài)的因子產(chǎn)品需指定溶劑復(fù)溶，請參考產(chǎn)品說明書【復(fù)融】方法，按照要求使用對應(yīng)溶劑溶解，大部分因子為無菌水復(fù)融，少部分需要無菌水以外的特殊溶劑溶解的產(chǎn)品則隨貨會贈送相應(yīng)的復(fù)融試劑；凍干粉溶解后如您需要進(jìn)一步稀釋分裝，需使用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存。 3、液體形式因子產(chǎn)品不需要復(fù)溶，具體濃度詳見產(chǎn)品標(biāo)簽，按照實驗需要用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存即可；
識別碼示意圖
