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產品概述
產品信息
| 別名 | GLIF, mMIF, GIF, Glycosylation-inhibiting factor |
| 物種 | Human |
| 表達宿主 | E.coli |
| 序列信息 | MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA with polyhistidine tag at the C-terminus. |
| 檢索號 | P14174.4 |
| 分子量 | 13.28 kDa |
| 表觀分子量 | 10 kDa |
| 標簽 | His-tag?at?the?C-terminus |
產品特性
| 純度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 內毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1%BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 運輸 | Ambient temperature or ice pack. |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. |
| 復融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介紹
Macrophage migration inhibitory factor (MIF or MMIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein that in humans is encoded by the MIF gene. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread and disease-free survival.
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