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產(chǎn)品概述
產(chǎn)品信息
| 名稱 | SU-DHL-1 (人間變性大細(xì)胞淋巴瘤細(xì)胞) (STR鑒定正確) |
| 別稱 | SU-DHL1; SUDHL1; SUDHL-1; SuDHL-1; SuDHL 1; Stanford University-Diffuse Histiocytic Lymphoma-1 |
| 種屬 | 人源 |
| 生長(zhǎng)特性 | 懸浮細(xì)胞 |
| 細(xì)胞形態(tài) | 淋巴母細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:2-1:6 |
| 推薦換液頻率 | 2-3次/周 |
參考資料(來(lái)源文獻(xiàn))
| 背景描述 | SU-DHL-1 is a histiocytic cell that was isolated from the lymph node of a White, 10-year-old, male patient with large cell lymphoma. The cells are surface Ig negative (sIG-). The cells are non-specific esterase, acid phosphatase and oil red O positive. The cells are periodic acid Schiff negative. These cells phagocytose Candida albicans and latex particles. The cells are reported to be very weakly E - rosette positive. ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. |
| 年齡（性別） | 男性；10歲 |
| 組織來(lái)源 | 淋巴結(jié) |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 淋巴瘤細(xì)胞 |
| 生物安全等級(jí) | BSL-1 |
| 倍增時(shí)間 | ~40-50 hours (DSMZ=ACC-356) |
| 受體表達(dá)情況 | CFS-1, expressed; (IL-1-R; IL-2-Rα; IL-6-R; TNFα-R; & c-fms), expressed; Surface Receptors: (Fc; IgMEAC; IgGEA; & E), not expressed |
| 抗原表達(dá)情況 | Monocyte Marker: CD163+ Lymphoid Marker: CD45- Progenitor Markers: CD10-, CD34- Activation Markers: CD30+, CD25+, CD70+, CD71+, CD80-, HLA-DR+, CD45- T-Cell Markers: CD2-, CD3-, CD4-, CD5+, CD7-, CD8- B-Cell Markers: CD19-, CD20-, CD21-, CD22- Myelom |
| 基因表達(dá)情況 | fusion gene NPM-ALK (p80)+; anaplastic lymphoma kinase (ALK)+; Ig not expressed |
| 保藏機(jī)構(gòu) | ATCC; CRL-2955 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X, Y CSF1PO 12 D2S1338 19,20 D3S1358 15,16 D5S818 11,12 D7S820 10,13 D8S1179 11 D13S317 9,13 D16S539 11 D18S51 15 D19S433 12,16 D21S11 29,30 FGA 21 PentaD 14 PentaE 11,15 TH01 6,7 TPOX 8 vWA 15,17 D6S1043 13 D12S391 17 D2S441 11,14 -
STR鑒定圖
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