TNF-R2/CD120b/TNFRSF1B (C-mFc), Human, Recombinant
英文名稱：TNF-R2/CD120b/TNFRSF1B (C-mFc), Human, Recombinant
貨號： PCK218
價格： ￥780 ￥2280 ￥11880 ￥17280
產品信息
| 產品形態(tài) | 粉末 |
| 產品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Tumor necrosis factor Receptor superfamily member 1B;TNFRSF1B;Tumor necrosis factorReceptor 2;TNF-R2;TNF-RII;Tumor necrosis factor Receptor type II;p75;p80 TNF-alphaReceptor;CD120b |
| 物種 | 人 |
| 表達宿主 | Human Cells |
| 序列信息 | Pro24-Thr206 |
| 檢索號 | P20333 |
| 分子量 | 46.5 kDa |
| 表觀分子量 | 60 kDa |
| 生物活性 | Immobilized Recombinant Human TNF RII-mFc at 1μg/mL (100 μL/well) can bind Recombinant Human TNF alpha-His. The ED50 of Recombinant Human TNF alpha-His is 1.97 ng/mL. |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 復融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | Tumor necrosis factor Receptor superfamily member 1B (TNFRSF1B) is a member of the tumor necrosisfactor Receptor superfamily. Human TNF RII contains four cysteinerich repeats in its ECD, which shares 58% and 56% amino acid sequence identity with the mouse and rat orthologs, respectively.TNF RII is expressedpredominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as onendothelial cells, microglia, astrocytes,neurons, oligodendrocytes, cardiac myocytes, thymocytes, andmesenchymal stem cells. TNF RII binds to the membranebound forms of TNFα and Lymphotoxinα/TNFβ; soluble TNF is thought to signal predominately through TNF RI.Soluble TNF RII is believed to inhibit TNFbiological activity by binding TNF thereby preventing it from activating membrane TNF Receptors. |
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Q1:官網的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q2:細胞因子用無菌水配成了100ug/mL母液，用于實驗時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進一步稀釋才行呢？
如果是直接做實驗，可以直接添加培養(yǎng)基進行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機離心的
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Q4:細胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
識別碼示意圖
