TGFα/ETGF/TGFA, Human, Recombinant
英文名稱：TGFα/ETGF/TGFA, Human, Recombinant
貨號： PCK283
價格： ￥420
規(guī)格：
10μg
產品信息
| 產品形態(tài) | 粉末 |
| 產品規(guī)格 | 10μg |
| 別名 | Sarcoma growth factor;TGF-type I;ETGF;TGFA |
| 物種 | 人 |
| 表達宿主 | E.coli |
| 序列信息 | MVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA with polyhistidine tag at the C-terminus. |
| 檢索號 | P01135.1 |
| 分子量 | 6.49 kDa |
| 表觀分子量 | 7 kDa |
| 標簽 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 0.2 ng/mL. The specific activity of recombinant human TGF alpha is > 5 × 10^6 IU/mg. |
| 純度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 內毒素 | < 0.1 EU per 1 μg of the protein by the LAL method. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0. |
| 復融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein. |
| 背景介紹 | Transforming growth factor alpha (TGF-α) is a protein that in humans is encoded by the TGFA gene. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes, and macrophages. |
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Q1:官網的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q2:細胞因子用無菌水配成了100ug/mL母液，用于實驗時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進一步稀釋才行呢？
如果是直接做實驗，可以直接添加培養(yǎng)基進行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機離心的
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Q4:細胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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