TGFβ 1/TGFB1, Mouse, Recombinant
英文名稱：TGFβ 1/TGFB1, Mouse, Recombinant
貨號： PCK357
價格： ￥680 ￥1680
規(guī)格：
10μg
50μg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg |
| 別名 | Differentiation inhibiting factor;Cartilage-inducing factor;Tgfb-1 |
| 物種 | 小鼠 |
| 表達(dá)宿主 | E.coli |
| 序列信息 | MALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS with polyhistidine tag at the C-terminus. |
| 檢索號 | P04202.1 (Mouse), P17246.1 (Rat) |
| 分子量 | 13.8 kDa |
| 表觀分子量 | 16 kDa |
| 標(biāo)簽 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells. The ED50 for this effect is < 0.1 ng/mL. |
| 純度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 內(nèi)毒素 | < 0.1 EU per 1 μg of the protein by the LAL method. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 運(yùn)輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein. |
| 背景介紹 | Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. In humans, TGF-β1 is encoded by the TGFB1 gene. |
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Q1:PCK357和PCK099這兩個都是TGFβ1有什么不一樣呢？
兩個的分子量不同，PCK357是有一個His標(biāo)簽的，PCK099沒有。
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Q2:官網(wǎng)的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q3:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實驗時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實驗，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q4:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機(jī)離心的
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Q5:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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