R-spondin 1/RSPO1, Human, Recombinant
英文名稱：R-spondin 1/RSPO1, Human, Recombinant
貨號： PCK214
價格： ￥780 ￥2280 ￥11880 ￥17280
規(guī)格：
10μg
50μg
500μg
1mg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | RSPO1;Roof plate-specific spondin-1 |
| 物種 | 人 |
| 表達(dá)宿主 | HEK293 |
| 序列信息 | A DNA sequence encoding Human R-Spondin 1 Protein (#Q2MKA7) (Ser21-Ala263) was expressed with polyhistidine-SUMO tag at the N-terminus. |
| 檢索號 | Q2MKA7 |
| 分子量 | 42.43 kDa |
| 表觀分子量 | 68 kDa |
| 標(biāo)簽 | His-SUMO tag at the N-terminus |
| 生物活性 | R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED50 for this effect is 1-3 μg/mL. |
| 純度 | > 95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg of the protein by the LAL method. |
| 儲存條件 | Store at -5~-20℃ or -80℃ for 6 months. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 運(yùn)輸條件 | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
| 有效期 | 12個月 |
| 制劑 | Liquid (1 × PBS, pH 7.4) |
| 背景介紹 | R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/6), subsequent clearing negative regulators ZNRF3/RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis. |
-
Q1:官網(wǎng)的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
-
Q2:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實驗時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實驗，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
-
Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機(jī)離心的
-
Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
注：百度瀏覽器無法支持掃一掃功能，請輸入識別碼，獲取相應(yīng)產(chǎn)品資料
識別碼示意圖
×
![]()
