IL-4Rα/CD124 (C-6His), Human, Recombinant
英文名稱：IL-4Rα/CD124 (C-6His), Human, Recombinant
貨號： PCK199
價格： ￥780 ￥2280 ￥9680 ￥13880
規(guī)格：
10μg
50μg
500μg
1mg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Interleukin-4 Receptor subunit alpha;IL-4 Receptor subunit alpha;IL-4R subunit alpha;IL-4R-alpha;IL-4RA;CD124;IL-4-binding Protein;IL4-BP;IL4R;IL4RA |
| 物種 | 人 |
| 表達(dá)宿主 | Human Cells |
| 序列信息 | Met26-His232 |
| 檢索號 | P24394 |
| 分子量 | 24.4 kDa |
| 表觀分子量 | 35-60 kDa |
| 標(biāo)簽 | C-6His |
| 生物活性 | Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 5-20 ng/mL. |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運(yùn)輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | Interleukin 4 Receptor alpha (IL4-Ra) is a widely expressed 140 kDa transmembrane glyco Protein in the class I Cytokine Receptor family. Mature human IL4-Ra consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a Cytokine binding region and one fibronectin type III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 Motif and one ITIM Motif. IL4-Ra plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. Soluble forms of IL4-Ra, generated by alternate splicing or proteolysis, retain Ligand binding properties and inhibit IL-4 bioactivity. IL4-Ra is a component of two distinct Receptor complexes and shows species selectivity between human and mouse. It can associate with the common gamma chain (γc) to form the IL-4 responsive type I Receptor in which γc increases the affinity for IL-4 and enables signaling. It can alternatively associate with IL13-Ra1 to form the type II Receptor which is responsive to both IL-4 and IL-13. The use of shared Receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other Cytokines that utilize γc. |
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Q1:官網(wǎng)的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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