IL-25 (C-6His), Human, Recombinant
英文名稱：IL-25 (C-6His), Human, Recombinant
貨號： PCK188
價(jià)格： ￥780 ￥2280 ￥11880 ￥17280
規(guī)格：
10μg
50μg
500μg
1mg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Interleukin-25;IL-25;Interleukin-17E;IL-17E;IL25;IL17E |
| 物種 | 人 |
| 表達(dá)宿主 | Human Cells |
| 序列信息 | Tyr33-Gly177 |
| 檢索號 | Q9H293 |
| 分子量 | 17.8 kDa |
| 表觀分子量 | 20-26 kDa |
| 標(biāo)簽 | C-6His |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲(chǔ)存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運(yùn)輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個(gè)月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 8.0. |
| 復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | Interleukin 25 (IL-25) belongs to the Interleukin 17 (IL-17) family of Proteins, which is comprised of six members (IL-17, IL-17B through IL-17F). These Proteins are secreted and are structurally related by sharing a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family Proteins are pro-inflammatory Cytokines that induce local Cytokine production and are involved in the regulation of immune functions. Human Interleukin-17E (IL17E), also referred to as Interleukin-25 (IL25), is a distinct member of the IL17 Cytokine family comprised of at least six members sharing a conserved cysteine-knot structure but divergent at the N-terminus. IL25 is a glyco Protein secreted as dimers by innate effector eosinophils and basophils, and present at very low levels in various peripheral tissues. IL25, together with IL17B, are Ligands for the Cytokine Receptor IL17BR, and the cross-linking induces NF-κB activation and production of the proinflammatory Chemokine IL-8, as well as ERK, JNK, and p38 activation. Overexpression of IL25 gene in transgenic mice suggested that this Cytokine can regulate hematopoietic and immune functions, and additionally is identified as a proinflammatory Cytokine favoring Th2-type immune responses possibly by enhancing the maintenance and functions of adaptive Th2 memory cells. |
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么？
我們公司的因子都是重組的，是不含動(dòng)物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時(shí)，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長時(shí)間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時(shí)間，再次使用時(shí)是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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