IL-2 (C-6His), Rat, Recombinant
英文名稱：IL-2 (C-6His), Rat, Recombinant
貨號： PCK026
價(jià)格： ￥580 ￥1680 ￥7080 ￥9680
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 液體 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Interleukin-2;IL-2;T-cell Growth Factor;TCGF;Aldesleukin;IL2 |
| 物種 | 大鼠 |
| 表達(dá)宿主 | Human Cells |
| 序列信息 | Ala21-Gln155 |
| 檢索號 | P17108 |
| 分子量 | 16.3 kDa |
| 表觀分子量 | 14-16 kDa |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲(chǔ)存條件 | Store at ≤-70℃, stable for 6 months after receipt. Store at ≤-70℃, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 運(yùn)輸條件 | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
| 有效期 | 6個(gè)月 |
| 制劑 | Supplied as a 0.2 μm filtered solution of 5 mM NaH?PO?, 5 mM Citric acid, 150 mM NaCl, pH 4.0. |
| 背景介紹 | Interleukin-2 (IL-2)is a O-glycosylated four α-helix bundle Cytokine that has potent stimulatory activity for antigenactivated T cells. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. Mature rat IL-2 shares 66% and 73% amino acid sequence identity with human and mouse IL-2,respectively. The Receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. |
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么？
我們公司的因子都是重組的，是不含動(dòng)物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時(shí)，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長時(shí)間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時(shí)間，再次使用時(shí)是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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Q5:收到細(xì)胞因子產(chǎn)品用什么溶劑復(fù)融？
1、細(xì)胞因子產(chǎn)品大部分為凍干形式發(fā)貨，少部分液體形式發(fā)貨； 2、凍干粉狀態(tài)的因子產(chǎn)品需指定溶劑復(fù)溶，請參考產(chǎn)品說明書【復(fù)融】方法，按照要求使用對應(yīng)溶劑溶解，大部分因子為無菌水復(fù)融，少部分需要無菌水以外的特殊溶劑溶解的產(chǎn)品則隨貨會(huì)贈(zèng)送相應(yīng)的復(fù)融試劑；凍干粉溶解后如您需要進(jìn)一步稀釋分裝，需使用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存。 3、液體形式因子產(chǎn)品不需要復(fù)溶，具體濃度詳見產(chǎn)品標(biāo)簽，按照實(shí)驗(yàn)需要用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存即可；
識別碼示意圖
