IL-1RL1/IL-1 R4 (C-6His), Mouse, Recombinant
英文名稱：IL-1RL1/IL-1 R4 (C-6His), Mouse, Recombinant
貨號(hào)： PCK241
價(jià)格： ￥780 ￥1880 ￥11880 ￥17280
規(guī)格：
10μg
50μg
500μg
1mg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Interleukin-1 Receptor-like 1;Lymphocyte antigen 84;Protein ST2;Protein T1;Il1rl1;DER4;Fit-1;IL-1 R4;IL-1RL1;IL-33R;Ly84;ST2L;ST2V;suppression of tumorigenicity 2 |
| 物種 | 小鼠 |
| 表達(dá)宿主 | Human Cells |
| 序列信息 | Ser27-Ala337 |
| 檢索號(hào) | P14719-2 |
| 分子量 | 36.6 kDa |
| 表觀分子量 | 55-70 kDa |
| 標(biāo)簽 | C-6His |
| 生物活性 | Immobilized Mouse ST2-His at 5 μg/mL (100 μL/well) can bind Rhesus Macaque IL-33-His. The ED50 of Rhesus Macaque IL-33-His is 82.03 ng/mL. |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲(chǔ)存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運(yùn)輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個(gè)月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | ST2, also called IL-1 R4, is an Interleukin-1 Receptor family glyco Protein that plays a role in Th2 immune responses. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. This Receptor is very similar to the IL-1 Receptor type I and the IL-18 Receptor α chain in that ST2 also has three extracellular Ig domains and an intracellular Toll domain. ST2 binds IL-33, enhances inflammatory Cytokines by activating nuclear factor-κB (NF-κB) and mitogen activated Protein (MAP) kinases. ST2 exists as either a membrane bound form (ST2L) or as a soluble form (sST2). ST2L acts as a transmembrane signalling Receptor for IL-33 by mediating the effect of IL-33 on the inflammatory process, while sST2 can suppress IL-33 activity. |
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么？
我們公司的因子都是重組的，是不含動(dòng)物源成分的。
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Q2:細(xì)胞因子用無(wú)菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時(shí)，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過(guò)PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長(zhǎng)時(shí)間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無(wú)菌水溶解后需要再次離心嗎？
加了無(wú)菌水后，如果沒(méi)有掛壁現(xiàn)象是不用離心的，如果您放置了一段時(shí)間，再次使用時(shí)是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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