GRO-α/CXCL1 (C-6His), Mouse, Recombinant
英文名稱：GRO-α/CXCL1 (C-6His), Mouse, Recombinant
貨號： PCK234
價格： ￥780 ￥2280 ￥11880 ￥17280
規(guī)格：
10μg
50μg
500μg
1mg
產品信息
| 產品形態(tài) | 粉末 |
| 產品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Growth-regulated alpha Protein;C-X-C Motif Chemokine 1;Platelet-Derived Growth Factor-inducible Protein KC;Secretory Protein N51;Cxcl1;Gro;Gro1;Mgsa;Scyb1 |
| 物種 | 小鼠 |
| 表達宿主 | Human Cells |
| 序列信息 | Arg20-Lys96 |
| 檢索號 | P12850 |
| 分子量 | 9.4 kDa |
| 表觀分子量 | 11-13 kDa |
| 標簽 | C-6His |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 復融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | Growth-regulated alpha Protein (CXCL1,KC), is a member of the alpha Chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-Derived Growth Factor. The N-terminal processed form KC (5-72) of the Protein is produced by proteolytic cleavage after secretion from bone marrow stromal cells, and shows a highly enhanced hematopoietic activity. Mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. Cxcl1 has chemotactic activity for neutrophils, and contributes to neutrophil activation during inflammation. Hematoregulatory Chemokine, in vitro, suppresses hematopoietic progenitor cell proliferation. |
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Q1:官網(wǎng)的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q2:細胞因子用無菌水配成了100ug/mL母液，用于實驗時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進一步稀釋才行呢？
如果是直接做實驗，可以直接添加培養(yǎng)基進行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機離心的
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Q4:細胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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